Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1569 and Pf6N2E2_1570 overlap by 22 nucleotides Pf6N2E2_1570 and Pf6N2E2_1571 are separated by 10 nucleotides
Pf6N2E2_1569 - FIG00957800: hypothetical protein, at 1,831,410 to 1,832,855
_1569
Pf6N2E2_1570 - Sll1359 protein, at 1,832,834 to 1,833,976
_1570
Pf6N2E2_1571 - FIG00966665: hypothetical protein, at 1,833,987 to 1,836,371
_1571
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1831.836 kb on + strand, within Pf6N2E2_1569 at 1831.836 kb on + strand, within Pf6N2E2_1569 at 1831.837 kb on - strand, within Pf6N2E2_1569 at 1831.998 kb on + strand, within Pf6N2E2_1569 at 1831.998 kb on + strand, within Pf6N2E2_1569 at 1832.065 kb on + strand, within Pf6N2E2_1569 at 1832.298 kb on + strand, within Pf6N2E2_1569 at 1832.298 kb on + strand, within Pf6N2E2_1569 at 1832.298 kb on + strand, within Pf6N2E2_1569 at 1832.299 kb on - strand, within Pf6N2E2_1569 at 1832.299 kb on - strand, within Pf6N2E2_1569 at 1832.299 kb on - strand, within Pf6N2E2_1569 at 1832.299 kb on - strand, within Pf6N2E2_1569 at 1832.375 kb on + strand, within Pf6N2E2_1569 at 1832.461 kb on - strand, within Pf6N2E2_1569 at 1832.463 kb on + strand, within Pf6N2E2_1569 at 1832.463 kb on + strand, within Pf6N2E2_1569 at 1832.463 kb on + strand, within Pf6N2E2_1569 at 1832.464 kb on - strand, within Pf6N2E2_1569 at 1832.464 kb on - strand, within Pf6N2E2_1569 at 1832.464 kb on - strand, within Pf6N2E2_1569 at 1832.467 kb on - strand, within Pf6N2E2_1569 at 1832.628 kb on + strand, within Pf6N2E2_1569 at 1832.629 kb on - strand, within Pf6N2E2_1569 at 1832.629 kb on - strand, within Pf6N2E2_1569 at 1832.918 kb on - strand at 1833.077 kb on + strand, within Pf6N2E2_1570 at 1833.108 kb on - strand, within Pf6N2E2_1570 at 1833.108 kb on - strand, within Pf6N2E2_1570 at 1833.164 kb on - strand, within Pf6N2E2_1570 at 1833.187 kb on + strand, within Pf6N2E2_1570 at 1833.270 kb on + strand, within Pf6N2E2_1570 at 1833.308 kb on + strand, within Pf6N2E2_1570 at 1833.329 kb on + strand, within Pf6N2E2_1570 at 1833.461 kb on + strand, within Pf6N2E2_1570 at 1833.462 kb on - strand, within Pf6N2E2_1570 at 1833.476 kb on + strand, within Pf6N2E2_1570 at 1833.476 kb on + strand, within Pf6N2E2_1570 at 1833.477 kb on - strand, within Pf6N2E2_1570 at 1833.497 kb on - strand, within Pf6N2E2_1570 at 1833.500 kb on + strand, within Pf6N2E2_1570 at 1833.512 kb on + strand, within Pf6N2E2_1570 at 1833.513 kb on - strand, within Pf6N2E2_1570 at 1833.596 kb on - strand, within Pf6N2E2_1570 at 1833.596 kb on - strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.714 kb on - strand, within Pf6N2E2_1570 at 1833.754 kb on - strand, within Pf6N2E2_1570 at 1833.995 kb on - strand at 1834.011 kb on - strand at 1834.015 kb on - strand at 1834.015 kb on - strand at 1834.028 kb on + strand at 1834.029 kb on - strand at 1834.029 kb on - strand at 1834.029 kb on - strand at 1834.068 kb on + strand at 1834.078 kb on - strand at 1834.203 kb on + strand at 1834.204 kb on - strand at 1834.204 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.216 kb on + strand at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.353 kb on + strand, within Pf6N2E2_1571 at 1834.354 kb on - strand, within Pf6N2E2_1571 at 1834.354 kb on - strand, within Pf6N2E2_1571 at 1834.479 kb on + strand at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.731 kb on + strand, within Pf6N2E2_1571 at 1834.732 kb on - strand, within Pf6N2E2_1571 at 1834.777 kb on - strand, within Pf6N2E2_1571 at 1834.777 kb on - strand, within Pf6N2E2_1571 at 1834.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,831,836 + Pf6N2E2_1569 0.29 -0.2 1,831,836 + Pf6N2E2_1569 0.29 -1.2 1,831,837 - Pf6N2E2_1569 0.30 -0.6 1,831,998 + Pf6N2E2_1569 0.41 +0.0 1,831,998 + Pf6N2E2_1569 0.41 +0.3 1,832,065 + Pf6N2E2_1569 0.45 +0.1 1,832,298 + Pf6N2E2_1569 0.61 -2.0 1,832,298 + Pf6N2E2_1569 0.61 -0.2 1,832,298 + Pf6N2E2_1569 0.61 +0.2 1,832,299 - Pf6N2E2_1569 0.61 +1.1 1,832,299 - Pf6N2E2_1569 0.61 +0.5 1,832,299 - Pf6N2E2_1569 0.61 +1.6 1,832,299 - Pf6N2E2_1569 0.61 +0.4 1,832,375 + Pf6N2E2_1569 0.67 +1.0 1,832,461 - Pf6N2E2_1569 0.73 -2.6 1,832,463 + Pf6N2E2_1569 0.73 +0.3 1,832,463 + Pf6N2E2_1569 0.73 +1.4 1,832,463 + Pf6N2E2_1569 0.73 +1.3 1,832,464 - Pf6N2E2_1569 0.73 +0.9 1,832,464 - Pf6N2E2_1569 0.73 -2.3 1,832,464 - Pf6N2E2_1569 0.73 -0.2 1,832,467 - Pf6N2E2_1569 0.73 +1.2 1,832,628 + Pf6N2E2_1569 0.84 -0.5 1,832,629 - Pf6N2E2_1569 0.84 -2.2 1,832,629 - Pf6N2E2_1569 0.84 -2.1 1,832,918 - -0.3 1,833,077 + Pf6N2E2_1570 0.21 -0.4 1,833,108 - Pf6N2E2_1570 0.24 +0.6 1,833,108 - Pf6N2E2_1570 0.24 -1.1 1,833,164 - Pf6N2E2_1570 0.29 -0.3 1,833,187 + Pf6N2E2_1570 0.31 +0.4 1,833,270 + Pf6N2E2_1570 0.38 -2.2 1,833,308 + Pf6N2E2_1570 0.41 -0.2 1,833,329 + Pf6N2E2_1570 0.43 +0.9 1,833,461 + Pf6N2E2_1570 0.55 +0.6 1,833,462 - Pf6N2E2_1570 0.55 +1.0 1,833,476 + Pf6N2E2_1570 0.56 -0.4 1,833,476 + Pf6N2E2_1570 0.56 +1.1 1,833,477 - Pf6N2E2_1570 0.56 -0.9 1,833,497 - Pf6N2E2_1570 0.58 +1.6 1,833,500 + Pf6N2E2_1570 0.58 +0.7 1,833,512 + Pf6N2E2_1570 0.59 -0.3 1,833,513 - Pf6N2E2_1570 0.59 +2.1 1,833,596 - Pf6N2E2_1570 0.67 -0.1 1,833,596 - Pf6N2E2_1570 0.67 -0.9 1,833,599 + Pf6N2E2_1570 0.67 -0.5 1,833,599 + Pf6N2E2_1570 0.67 -0.3 1,833,599 + Pf6N2E2_1570 0.67 -1.4 1,833,599 + Pf6N2E2_1570 0.67 -0.5 1,833,600 - Pf6N2E2_1570 0.67 +1.6 1,833,600 - Pf6N2E2_1570 0.67 +0.8 1,833,600 - Pf6N2E2_1570 0.67 -1.0 1,833,600 - Pf6N2E2_1570 0.67 +1.3 1,833,714 - Pf6N2E2_1570 0.77 +0.7 1,833,754 - Pf6N2E2_1570 0.80 -0.4 1,833,995 - +0.6 1,834,011 - -0.2 1,834,015 - +1.6 1,834,015 - -1.2 1,834,028 + -0.1 1,834,029 - -0.8 1,834,029 - -2.1 1,834,029 - -0.4 1,834,068 + -1.0 1,834,078 - +0.1 1,834,203 + -0.7 1,834,204 - -0.9 1,834,204 - -0.1 1,834,206 - +0.6 1,834,206 - -0.5 1,834,206 - -0.3 1,834,206 - +1.3 1,834,206 - +0.6 1,834,216 + +1.0 1,834,248 + Pf6N2E2_1571 0.11 +0.0 1,834,248 + Pf6N2E2_1571 0.11 +0.9 1,834,248 + Pf6N2E2_1571 0.11 +0.6 1,834,248 + Pf6N2E2_1571 0.11 -0.4 1,834,249 - Pf6N2E2_1571 0.11 -0.6 1,834,249 - Pf6N2E2_1571 0.11 +1.0 1,834,249 - Pf6N2E2_1571 0.11 -0.1 1,834,249 - Pf6N2E2_1571 0.11 -0.1 1,834,249 - Pf6N2E2_1571 0.11 -0.1 1,834,249 - Pf6N2E2_1571 0.11 +0.6 1,834,303 - Pf6N2E2_1571 0.13 -0.4 1,834,303 - Pf6N2E2_1571 0.13 -1.7 1,834,303 - Pf6N2E2_1571 0.13 +0.8 1,834,353 + Pf6N2E2_1571 0.15 -0.8 1,834,354 - Pf6N2E2_1571 0.15 -1.1 1,834,354 - Pf6N2E2_1571 0.15 -0.9 1,834,479 + +3.4 1,834,593 + Pf6N2E2_1571 0.25 -0.1 1,834,593 + Pf6N2E2_1571 0.25 -1.1 1,834,593 + Pf6N2E2_1571 0.25 -1.7 1,834,593 + Pf6N2E2_1571 0.25 +0.9 1,834,593 + Pf6N2E2_1571 0.25 -0.1 1,834,593 + Pf6N2E2_1571 0.25 +0.7 1,834,594 - Pf6N2E2_1571 0.25 +0.8 1,834,594 - Pf6N2E2_1571 0.25 -0.8 1,834,594 - Pf6N2E2_1571 0.25 -0.1 1,834,594 - Pf6N2E2_1571 0.25 +1.5 1,834,594 - Pf6N2E2_1571 0.25 -1.2 1,834,731 + Pf6N2E2_1571 0.31 -1.0 1,834,732 - Pf6N2E2_1571 0.31 +1.7 1,834,777 - Pf6N2E2_1571 0.33 +0.3 1,834,777 - Pf6N2E2_1571 0.33 +0.8 1,834,969 + +0.7
Or see this region's nucleotide sequence