Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1168

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1166 and Pf6N2E2_1167 are separated by 77 nucleotidesPf6N2E2_1167 and Pf6N2E2_1168 are separated by 61 nucleotidesPf6N2E2_1168 and Pf6N2E2_1169 are separated by 27 nucleotides Pf6N2E2_1166 - Mobile element protein, at 1,367,174 to 1,367,464 _1166 Pf6N2E2_1167 - Transposase, at 1,367,542 to 1,367,838 _1167 Pf6N2E2_1168 - Transposase, at 1,367,900 to 1,368,298 _1168 Pf6N2E2_1169 - TnpR protein, at 1,368,326 to 1,368,886 _1169 Position (kb) 1367 1368 1369Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1366.937 kb on + strandat 1366.941 kb on + strandat 1366.964 kb on + strandat 1367.011 kb on + strandat 1367.021 kb on + strandat 1367.133 kb on + strandat 1367.311 kb on - strand, within Pf6N2E2_1166at 1367.489 kb on - strandat 1367.520 kb on + strandat 1367.520 kb on + strandat 1367.521 kb on - strandat 1367.979 kb on - strand, within Pf6N2E2_1168at 1368.093 kb on - strand, within Pf6N2E2_1168at 1368.141 kb on - strand, within Pf6N2E2_1168at 1368.142 kb on + strand, within Pf6N2E2_1168at 1368.188 kb on + strand, within Pf6N2E2_1168at 1368.189 kb on - strand, within Pf6N2E2_1168at 1368.189 kb on - strand, within Pf6N2E2_1168at 1368.189 kb on - strand, within Pf6N2E2_1168at 1368.368 kb on - strandat 1368.578 kb on + strand, within Pf6N2E2_1169at 1368.578 kb on + strand, within Pf6N2E2_1169at 1368.579 kb on - strand, within Pf6N2E2_1169at 1368.599 kb on + strand, within Pf6N2E2_1169at 1368.982 kb on + strandat 1369.230 kb on + strandat 1369.295 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,366,937 + +0.1
1,366,941 + -0.1
1,366,964 + -1.7
1,367,011 + -0.1
1,367,021 + -1.9
1,367,133 + +1.6
1,367,311 - Pf6N2E2_1166 0.47 -0.3
1,367,489 - -1.1
1,367,520 + -1.0
1,367,520 + -0.6
1,367,521 - +0.5
1,367,979 - Pf6N2E2_1168 0.20 +0.1
1,368,093 - Pf6N2E2_1168 0.48 +1.3
1,368,141 - Pf6N2E2_1168 0.60 +1.5
1,368,142 + Pf6N2E2_1168 0.61 -0.1
1,368,188 + Pf6N2E2_1168 0.72 +0.9
1,368,189 - Pf6N2E2_1168 0.72 +1.0
1,368,189 - Pf6N2E2_1168 0.72 +0.0
1,368,189 - Pf6N2E2_1168 0.72 +1.6
1,368,368 - +0.1
1,368,578 + Pf6N2E2_1169 0.45 -0.8
1,368,578 + Pf6N2E2_1169 0.45 +1.1
1,368,579 - Pf6N2E2_1169 0.45 +0.4
1,368,599 + Pf6N2E2_1169 0.49 +2.9
1,368,982 + +3.6
1,369,230 + +2.1
1,369,295 + +0.2

Or see this region's nucleotide sequence