Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1072 and Pf6N2E2_1073 are separated by 250 nucleotides Pf6N2E2_1073 and Pf6N2E2_1074 are separated by 229 nucleotides Pf6N2E2_1074 and Pf6N2E2_1075 are separated by 20 nucleotides
Pf6N2E2_1072 - LmbE-like protein, at 1,250,862 to 1,251,626
_1072
Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036
_1073
Pf6N2E2_1074 - FIG00953356: hypothetical protein, at 1,254,266 to 1,254,619
_1074
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Position (kb)
1251
1252
1253
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.646 kb on - strand at 1251.674 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.864 kb on + strand at 1251.874 kb on - strand at 1251.936 kb on - strand at 1252.072 kb on + strand at 1252.073 kb on - strand at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.389 kb on + strand, within Pf6N2E2_1073 at 1252.441 kb on - strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073 at 1252.667 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.081 kb on + strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.082 kb on - strand, within Pf6N2E2_1073 at 1253.084 kb on + strand, within Pf6N2E2_1073 at 1253.084 kb on + strand, within Pf6N2E2_1073 at 1253.114 kb on + strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.115 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.184 kb on - strand, within Pf6N2E2_1073 at 1253.270 kb on + strand, within Pf6N2E2_1073 at 1253.271 kb on - strand, within Pf6N2E2_1073 at 1253.306 kb on + strand, within Pf6N2E2_1073 at 1253.306 kb on + strand, within Pf6N2E2_1073 at 1253.307 kb on - strand, within Pf6N2E2_1073 at 1253.315 kb on + strand, within Pf6N2E2_1073 at 1253.315 kb on + strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.316 kb on - strand, within Pf6N2E2_1073 at 1253.446 kb on - strand at 1253.507 kb on + strand, within Pf6N2E2_1073 at 1253.507 kb on + strand, within Pf6N2E2_1073 at 1253.765 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.819 kb on + strand, within Pf6N2E2_1073 at 1253.834 kb on + strand at 1253.834 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.037 kb on - strand at 1254.037 kb on - strand at 1254.132 kb on + strand at 1254.133 kb on - strand at 1254.133 kb on - strand at 1254.560 kb on - strand, within Pf6N2E2_1074 at 1254.583 kb on - strand, within Pf6N2E2_1074 at 1254.850 kb on + strand at 1254.850 kb on + strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.956 kb on - strand, within Pf6N2E2_1075
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,250,965 + Pf6N2E2_1072 0.13 -0.3 1,250,965 + Pf6N2E2_1072 0.13 -0.8 1,250,965 + Pf6N2E2_1072 0.13 -0.6 1,250,966 - Pf6N2E2_1072 0.14 -1.8 1,250,966 - Pf6N2E2_1072 0.14 +0.2 1,250,966 - Pf6N2E2_1072 0.14 -1.1 1,250,971 + Pf6N2E2_1072 0.14 +0.5 1,250,971 + Pf6N2E2_1072 0.14 -2.3 1,250,971 + Pf6N2E2_1072 0.14 -2.4 1,250,972 - Pf6N2E2_1072 0.14 -0.4 1,250,972 - Pf6N2E2_1072 0.14 -2.0 1,250,972 - Pf6N2E2_1072 0.14 -0.9 1,251,351 - Pf6N2E2_1072 0.64 +1.3 1,251,351 - Pf6N2E2_1072 0.64 +0.1 1,251,351 - Pf6N2E2_1072 0.64 +1.0 1,251,351 - Pf6N2E2_1072 0.64 -2.9 1,251,646 - +2.8 1,251,674 + -0.4 1,251,750 + -1.5 1,251,750 + -1.1 1,251,750 + -0.6 1,251,750 + +0.9 1,251,751 - +3.0 1,251,751 - +2.3 1,251,751 - +0.0 1,251,864 + +1.6 1,251,874 - +0.3 1,251,936 - +0.8 1,252,072 + -1.7 1,252,073 - -1.5 1,252,238 + Pf6N2E2_1073 0.17 +1.6 1,252,238 + Pf6N2E2_1073 0.17 -1.7 1,252,238 + Pf6N2E2_1073 0.17 -1.7 1,252,238 + Pf6N2E2_1073 0.17 +0.3 1,252,238 + Pf6N2E2_1073 0.17 -2.2 1,252,239 - Pf6N2E2_1073 0.17 -0.1 1,252,239 - Pf6N2E2_1073 0.17 +0.6 1,252,239 - Pf6N2E2_1073 0.17 +0.6 1,252,239 - Pf6N2E2_1073 0.17 -0.2 1,252,239 - Pf6N2E2_1073 0.17 +0.6 1,252,239 - Pf6N2E2_1073 0.17 -0.2 1,252,239 - Pf6N2E2_1073 0.17 +0.7 1,252,239 - Pf6N2E2_1073 0.17 -1.4 1,252,239 - Pf6N2E2_1073 0.17 -0.9 1,252,239 - Pf6N2E2_1073 0.17 +0.7 1,252,239 - Pf6N2E2_1073 0.17 -1.7 1,252,239 - Pf6N2E2_1073 0.17 +0.8 1,252,239 - Pf6N2E2_1073 0.17 -1.2 1,252,379 + Pf6N2E2_1073 0.23 -0.8 1,252,379 + Pf6N2E2_1073 0.23 +0.4 1,252,380 - Pf6N2E2_1073 0.23 -1.2 1,252,380 - Pf6N2E2_1073 0.23 -1.5 1,252,389 + Pf6N2E2_1073 0.24 +0.3 1,252,441 - Pf6N2E2_1073 0.26 +0.8 1,252,607 + Pf6N2E2_1073 0.34 -0.8 1,252,607 + Pf6N2E2_1073 0.34 -0.3 1,252,608 - Pf6N2E2_1073 0.34 -1.8 1,252,608 - Pf6N2E2_1073 0.34 +0.5 1,252,667 + Pf6N2E2_1073 0.37 -0.5 1,253,081 + Pf6N2E2_1073 0.56 +0.5 1,253,081 + Pf6N2E2_1073 0.56 -0.8 1,253,081 + Pf6N2E2_1073 0.56 +0.4 1,253,081 + Pf6N2E2_1073 0.56 -0.5 1,253,081 + Pf6N2E2_1073 0.56 +0.3 1,253,081 + Pf6N2E2_1073 0.56 +1.5 1,253,081 + Pf6N2E2_1073 0.56 -0.6 1,253,081 + Pf6N2E2_1073 0.56 -0.1 1,253,081 + Pf6N2E2_1073 0.56 -0.4 1,253,082 - Pf6N2E2_1073 0.56 +0.1 1,253,082 - Pf6N2E2_1073 0.56 -0.5 1,253,082 - Pf6N2E2_1073 0.56 -1.2 1,253,082 - Pf6N2E2_1073 0.56 -0.1 1,253,082 - Pf6N2E2_1073 0.56 -0.7 1,253,082 - Pf6N2E2_1073 0.56 -1.6 1,253,082 - Pf6N2E2_1073 0.56 +0.8 1,253,084 + Pf6N2E2_1073 0.56 +0.4 1,253,084 + Pf6N2E2_1073 0.56 -0.3 1,253,114 + Pf6N2E2_1073 0.57 -0.1 1,253,115 - Pf6N2E2_1073 0.57 +2.9 1,253,115 - Pf6N2E2_1073 0.57 -0.2 1,253,115 - Pf6N2E2_1073 0.57 -0.5 1,253,184 - Pf6N2E2_1073 0.61 -0.2 1,253,184 - Pf6N2E2_1073 0.61 +0.9 1,253,184 - Pf6N2E2_1073 0.61 -1.7 1,253,184 - Pf6N2E2_1073 0.61 -0.4 1,253,270 + Pf6N2E2_1073 0.64 -0.1 1,253,271 - Pf6N2E2_1073 0.65 -0.8 1,253,306 + Pf6N2E2_1073 0.66 -0.4 1,253,306 + Pf6N2E2_1073 0.66 -0.1 1,253,307 - Pf6N2E2_1073 0.66 +0.9 1,253,315 + Pf6N2E2_1073 0.67 -1.4 1,253,315 + Pf6N2E2_1073 0.67 -0.3 1,253,316 - Pf6N2E2_1073 0.67 -1.1 1,253,316 - Pf6N2E2_1073 0.67 +1.4 1,253,316 - Pf6N2E2_1073 0.67 +0.4 1,253,316 - Pf6N2E2_1073 0.67 -0.8 1,253,446 - +1.9 1,253,507 + Pf6N2E2_1073 0.75 -0.9 1,253,507 + Pf6N2E2_1073 0.75 -0.5 1,253,765 + Pf6N2E2_1073 0.87 +0.8 1,253,780 + Pf6N2E2_1073 0.88 -0.7 1,253,780 + Pf6N2E2_1073 0.88 -0.4 1,253,780 + Pf6N2E2_1073 0.88 -0.3 1,253,780 + Pf6N2E2_1073 0.88 +1.9 1,253,780 + Pf6N2E2_1073 0.88 -0.3 1,253,780 + Pf6N2E2_1073 0.88 -1.0 1,253,780 + Pf6N2E2_1073 0.88 +0.5 1,253,780 + Pf6N2E2_1073 0.88 -1.5 1,253,780 + Pf6N2E2_1073 0.88 -2.2 1,253,781 - Pf6N2E2_1073 0.88 -2.1 1,253,781 - Pf6N2E2_1073 0.88 +1.7 1,253,781 - Pf6N2E2_1073 0.88 -0.1 1,253,819 + Pf6N2E2_1073 0.90 -0.3 1,253,834 + -0.5 1,253,834 + +0.1 1,254,036 + +0.2 1,254,036 + +0.2 1,254,036 + +0.4 1,254,036 + -1.8 1,254,036 + -0.3 1,254,037 - -1.2 1,254,037 - -1.1 1,254,132 + +1.2 1,254,133 - +0.8 1,254,133 - +0.6 1,254,560 - Pf6N2E2_1074 0.83 -0.7 1,254,583 - Pf6N2E2_1074 0.90 +0.0 1,254,850 + +0.7 1,254,850 + +0.3 1,254,851 - +0.7 1,254,851 - +0.1 1,254,851 - -2.3 1,254,955 + Pf6N2E2_1075 0.11 -0.8 1,254,955 + Pf6N2E2_1075 0.11 -0.4 1,254,956 - Pf6N2E2_1075 0.11 -2.5
Or see this region's nucleotide sequence