Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1059

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1058 and Pf6N2E2_1059 are separated by 90 nucleotidesPf6N2E2_1059 and Pf6N2E2_1060 are separated by 175 nucleotidesPf6N2E2_1060 and Pf6N2E2_1061 overlap by 26 nucleotides Pf6N2E2_1058 - FIG000557: hypothetical protein co-occurring with RecR, at 1,233,980 to 1,235,377 _1058 Pf6N2E2_1059 - Phosphoglucomutase (EC 5.4.2.2), at 1,235,468 to 1,237,114 _1059 Pf6N2E2_1060 - Pirin, at 1,237,290 to 1,237,985 _1060 Pf6N2E2_1061 - hypothetical protein, at 1,237,960 to 1,238,091 _1061 Position (kb) 1235 1236 1237 1238Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1234.481 kb on + strand, within Pf6N2E2_1058at 1234.481 kb on + strand, within Pf6N2E2_1058at 1234.481 kb on + strand, within Pf6N2E2_1058at 1234.481 kb on + strand, within Pf6N2E2_1058at 1234.481 kb on + strand, within Pf6N2E2_1058at 1234.482 kb on - strand, within Pf6N2E2_1058at 1234.482 kb on - strand, within Pf6N2E2_1058at 1234.482 kb on - strandat 1234.816 kb on + strand, within Pf6N2E2_1058at 1234.886 kb on + strand, within Pf6N2E2_1058at 1234.925 kb on + strand, within Pf6N2E2_1058at 1234.926 kb on - strand, within Pf6N2E2_1058at 1234.932 kb on - strand, within Pf6N2E2_1058at 1235.030 kb on + strand, within Pf6N2E2_1058at 1235.276 kb on + strandat 1235.277 kb on - strandat 1235.277 kb on - strandat 1235.287 kb on + strandat 1235.287 kb on + strandat 1235.287 kb on + strandat 1235.287 kb on + strandat 1235.287 kb on + strandat 1235.287 kb on + strandat 1235.288 kb on - strandat 1235.376 kb on - strandat 1235.439 kb on + strandat 1235.546 kb on + strandat 1235.547 kb on - strandat 1235.547 kb on - strandat 1235.547 kb on - strandat 1235.547 kb on - strandat 1235.769 kb on + strand, within Pf6N2E2_1059at 1235.769 kb on + strand, within Pf6N2E2_1059at 1235.770 kb on - strand, within Pf6N2E2_1059at 1235.770 kb on - strand, within Pf6N2E2_1059at 1235.794 kb on - strand, within Pf6N2E2_1059at 1235.816 kb on + strand, within Pf6N2E2_1059at 1235.816 kb on + strand, within Pf6N2E2_1059at 1235.855 kb on + strand, within Pf6N2E2_1059at 1235.939 kb on + strand, within Pf6N2E2_1059at 1235.939 kb on + strand, within Pf6N2E2_1059at 1235.940 kb on - strand, within Pf6N2E2_1059at 1235.940 kb on - strand, within Pf6N2E2_1059at 1235.948 kb on - strand, within Pf6N2E2_1059at 1235.975 kb on - strand, within Pf6N2E2_1059at 1236.089 kb on + strand, within Pf6N2E2_1059at 1236.089 kb on + strand, within Pf6N2E2_1059at 1236.089 kb on + strand, within Pf6N2E2_1059at 1236.090 kb on - strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.093 kb on + strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.094 kb on - strand, within Pf6N2E2_1059at 1236.101 kb on + strand, within Pf6N2E2_1059at 1236.102 kb on - strand, within Pf6N2E2_1059at 1236.102 kb on - strand, within Pf6N2E2_1059at 1236.222 kb on - strand, within Pf6N2E2_1059at 1236.443 kb on + strand, within Pf6N2E2_1059at 1236.443 kb on + strand, within Pf6N2E2_1059at 1236.443 kb on + strand, within Pf6N2E2_1059at 1236.444 kb on - strand, within Pf6N2E2_1059at 1236.465 kb on - strand, within Pf6N2E2_1059at 1236.572 kb on + strand, within Pf6N2E2_1059at 1236.573 kb on - strand, within Pf6N2E2_1059at 1236.573 kb on - strand, within Pf6N2E2_1059at 1236.573 kb on - strand, within Pf6N2E2_1059at 1236.913 kb on + strand, within Pf6N2E2_1059at 1237.012 kb on + strandat 1237.012 kb on + strandat 1237.012 kb on + strandat 1237.012 kb on + strandat 1237.013 kb on - strandat 1237.013 kb on - strandat 1237.016 kb on + strandat 1237.016 kb on + strandat 1237.016 kb on + strandat 1237.016 kb on + strandat 1237.016 kb on + strandat 1237.016 kb on + strandat 1237.017 kb on - strandat 1237.017 kb on - strandat 1237.017 kb on - strandat 1237.017 kb on - strandat 1237.017 kb on - strandat 1237.025 kb on + strandat 1237.026 kb on - strandat 1237.099 kb on + strandat 1237.115 kb on + strandat 1237.116 kb on - strandat 1237.116 kb on - strandat 1237.221 kb on - strandat 1237.344 kb on + strandat 1237.456 kb on + strand, within Pf6N2E2_1060at 1237.638 kb on + strand, within Pf6N2E2_1060at 1237.638 kb on + strand, within Pf6N2E2_1060at 1237.639 kb on - strand, within Pf6N2E2_1060at 1237.639 kb on - strand, within Pf6N2E2_1060at 1237.675 kb on + strand, within Pf6N2E2_1060at 1237.675 kb on + strand, within Pf6N2E2_1060at 1237.676 kb on - strand, within Pf6N2E2_1060at 1237.676 kb on - strand, within Pf6N2E2_1060at 1237.676 kb on - strand, within Pf6N2E2_1060at 1237.676 kb on - strand, within Pf6N2E2_1060at 1237.676 kb on - strand, within Pf6N2E2_1060at 1237.716 kb on + strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.717 kb on - strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.786 kb on + strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.787 kb on - strand, within Pf6N2E2_1060at 1237.900 kb on + strand, within Pf6N2E2_1060at 1237.991 kb on + strand, within Pf6N2E2_1061at 1238.031 kb on + strand, within Pf6N2E2_1061

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,234,481 + Pf6N2E2_1058 0.36 +0.2
1,234,481 + Pf6N2E2_1058 0.36 +2.1
1,234,481 + Pf6N2E2_1058 0.36 -1.5
1,234,481 + Pf6N2E2_1058 0.36 +0.3
1,234,481 + Pf6N2E2_1058 0.36 +0.8
1,234,482 - Pf6N2E2_1058 0.36 -1.6
1,234,482 - Pf6N2E2_1058 0.36 +0.2
1,234,482 - +0.3
1,234,816 + Pf6N2E2_1058 0.60 +0.6
1,234,886 + Pf6N2E2_1058 0.65 -1.5
1,234,925 + Pf6N2E2_1058 0.68 +0.4
1,234,926 - Pf6N2E2_1058 0.68 +0.1
1,234,932 - Pf6N2E2_1058 0.68 -0.7
1,235,030 + Pf6N2E2_1058 0.75 +0.4
1,235,276 + -1.0
1,235,277 - +1.0
1,235,277 - -1.6
1,235,287 + -0.3
1,235,287 + +3.1
1,235,287 + +0.0
1,235,287 + +2.8
1,235,287 + -0.0
1,235,287 + -0.2
1,235,288 - +0.5
1,235,376 - +1.6
1,235,439 + -0.2
1,235,546 + -0.2
1,235,547 - -0.0
1,235,547 - -1.8
1,235,547 - -1.1
1,235,547 - +1.3
1,235,769 + Pf6N2E2_1059 0.18 +0.6
1,235,769 + Pf6N2E2_1059 0.18 -0.8
1,235,770 - Pf6N2E2_1059 0.18 -0.5
1,235,770 - Pf6N2E2_1059 0.18 -0.0
1,235,794 - Pf6N2E2_1059 0.20 -0.9
1,235,816 + Pf6N2E2_1059 0.21 -0.1
1,235,816 + Pf6N2E2_1059 0.21 -0.6
1,235,855 + Pf6N2E2_1059 0.23 -0.5
1,235,939 + Pf6N2E2_1059 0.29 -0.9
1,235,939 + Pf6N2E2_1059 0.29 -0.6
1,235,940 - Pf6N2E2_1059 0.29 -2.6
1,235,940 - Pf6N2E2_1059 0.29 -2.4
1,235,948 - Pf6N2E2_1059 0.29 +0.9
1,235,975 - Pf6N2E2_1059 0.31 +1.2
1,236,089 + Pf6N2E2_1059 0.38 +0.6
1,236,089 + Pf6N2E2_1059 0.38 +1.2
1,236,089 + Pf6N2E2_1059 0.38 -1.4
1,236,090 - Pf6N2E2_1059 0.38 -2.3
1,236,093 + Pf6N2E2_1059 0.38 -0.3
1,236,093 + Pf6N2E2_1059 0.38 -0.8
1,236,093 + Pf6N2E2_1059 0.38 -0.8
1,236,093 + Pf6N2E2_1059 0.38 +3.1
1,236,093 + Pf6N2E2_1059 0.38 +0.8
1,236,093 + Pf6N2E2_1059 0.38 -1.2
1,236,093 + Pf6N2E2_1059 0.38 +0.9
1,236,093 + Pf6N2E2_1059 0.38 +0.7
1,236,094 - Pf6N2E2_1059 0.38 -1.0
1,236,094 - Pf6N2E2_1059 0.38 +0.6
1,236,094 - Pf6N2E2_1059 0.38 -2.4
1,236,094 - Pf6N2E2_1059 0.38 +0.2
1,236,094 - Pf6N2E2_1059 0.38 -0.7
1,236,094 - Pf6N2E2_1059 0.38 -0.5
1,236,094 - Pf6N2E2_1059 0.38 +0.3
1,236,101 + Pf6N2E2_1059 0.38 -2.1
1,236,102 - Pf6N2E2_1059 0.38 -0.2
1,236,102 - Pf6N2E2_1059 0.38 +0.1
1,236,222 - Pf6N2E2_1059 0.46 -1.1
1,236,443 + Pf6N2E2_1059 0.59 -0.0
1,236,443 + Pf6N2E2_1059 0.59 -0.3
1,236,443 + Pf6N2E2_1059 0.59 +0.1
1,236,444 - Pf6N2E2_1059 0.59 -0.9
1,236,465 - Pf6N2E2_1059 0.61 -1.1
1,236,572 + Pf6N2E2_1059 0.67 -0.8
1,236,573 - Pf6N2E2_1059 0.67 -0.4
1,236,573 - Pf6N2E2_1059 0.67 +1.0
1,236,573 - Pf6N2E2_1059 0.67 +0.1
1,236,913 + Pf6N2E2_1059 0.88 -1.4
1,237,012 + -1.1
1,237,012 + +1.5
1,237,012 + +1.8
1,237,012 + -0.2
1,237,013 - -0.1
1,237,013 - -0.8
1,237,016 + -0.8
1,237,016 + +0.2
1,237,016 + -0.3
1,237,016 + +0.4
1,237,016 + -0.3
1,237,016 + +0.7
1,237,017 - +0.6
1,237,017 - -0.2
1,237,017 - +0.6
1,237,017 - +0.0
1,237,017 - +0.2
1,237,025 + +0.9
1,237,026 - -1.5
1,237,099 + +0.4
1,237,115 + -0.7
1,237,116 - -0.6
1,237,116 - +0.6
1,237,221 - -0.5
1,237,344 + +2.9
1,237,456 + Pf6N2E2_1060 0.24 -1.1
1,237,638 + Pf6N2E2_1060 0.50 -1.0
1,237,638 + Pf6N2E2_1060 0.50 +0.6
1,237,639 - Pf6N2E2_1060 0.50 -0.6
1,237,639 - Pf6N2E2_1060 0.50 +1.2
1,237,675 + Pf6N2E2_1060 0.55 -0.6
1,237,675 + Pf6N2E2_1060 0.55 +0.2
1,237,676 - Pf6N2E2_1060 0.55 -0.5
1,237,676 - Pf6N2E2_1060 0.55 +1.6
1,237,676 - Pf6N2E2_1060 0.55 +0.1
1,237,676 - Pf6N2E2_1060 0.55 -0.4
1,237,676 - Pf6N2E2_1060 0.55 -1.4
1,237,716 + Pf6N2E2_1060 0.61 -0.9
1,237,717 - Pf6N2E2_1060 0.61 +0.2
1,237,717 - Pf6N2E2_1060 0.61 +0.7
1,237,717 - Pf6N2E2_1060 0.61 -1.4
1,237,717 - Pf6N2E2_1060 0.61 -0.9
1,237,717 - Pf6N2E2_1060 0.61 +0.4
1,237,717 - Pf6N2E2_1060 0.61 +0.9
1,237,717 - Pf6N2E2_1060 0.61 +0.3
1,237,717 - Pf6N2E2_1060 0.61 -0.8
1,237,786 + Pf6N2E2_1060 0.71 -1.5
1,237,786 + Pf6N2E2_1060 0.71 -1.5
1,237,786 + Pf6N2E2_1060 0.71 +0.5
1,237,786 + Pf6N2E2_1060 0.71 -0.8
1,237,786 + Pf6N2E2_1060 0.71 +0.6
1,237,786 + Pf6N2E2_1060 0.71 -2.0
1,237,786 + Pf6N2E2_1060 0.71 -0.2
1,237,786 + Pf6N2E2_1060 0.71 -0.7
1,237,787 - Pf6N2E2_1060 0.71 +0.0
1,237,787 - Pf6N2E2_1060 0.71 +0.2
1,237,787 - Pf6N2E2_1060 0.71 +1.0
1,237,787 - Pf6N2E2_1060 0.71 +1.9
1,237,787 - Pf6N2E2_1060 0.71 +0.9
1,237,787 - Pf6N2E2_1060 0.71 -0.3
1,237,787 - Pf6N2E2_1060 0.71 +0.2
1,237,787 - Pf6N2E2_1060 0.71 +0.6
1,237,787 - Pf6N2E2_1060 0.71 -0.4
1,237,787 - Pf6N2E2_1060 0.71 -1.1
1,237,787 - Pf6N2E2_1060 0.71 +0.5
1,237,900 + Pf6N2E2_1060 0.88 -0.0
1,237,991 + Pf6N2E2_1061 0.23 +0.6
1,238,031 + Pf6N2E2_1061 0.54 +0.5

Or see this region's nucleotide sequence