Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19210 and ABZR86_RS19215 are separated by 190 nucleotides ABZR86_RS19215 and ABZR86_RS19220 are separated by 60 nucleotides ABZR86_RS19220 and ABZR86_RS19225 are separated by 44 nucleotides
ABZR86_RS19210: ABZR86_RS19210 - polyhydroxyalkanoate depolymerase, at 296,712 to 297,965
_RS19210
ABZR86_RS19215: ABZR86_RS19215 - class I SAM-dependent methyltransferase, at 298,156 to 298,902
_RS19215
ABZR86_RS19220: ABZR86_RS19220 - RDD family protein, at 298,963 to 299,469
_RS19220
ABZR86_RS19225: ABZR86_RS19225 - site-specific tyrosine recombinase XerD, at 299,514 to 300,425
_RS19225
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1 at 298.001 kb on + strand at 298.001 kb on + strand at 298.053 kb on + strand at 298.054 kb on - strand at 298.070 kb on + strand at 298.116 kb on + strand at 298.116 kb on + strand at 298.157 kb on + strand at 298.158 kb on - strand at 298.158 kb on - strand at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.307 kb on + strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.355 kb on + strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.605 kb on - strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.836 kb on - strand at 298.836 kb on - strand at 298.836 kb on - strand at 298.908 kb on + strand at 298.909 kb on - strand at 299.255 kb on + strand, within ABZR86_RS19220 at 299.255 kb on + strand, within ABZR86_RS19220 at 299.256 kb on - strand, within ABZR86_RS19220 at 299.289 kb on - strand, within ABZR86_RS19220 at 299.357 kb on + strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.399 kb on + strand, within ABZR86_RS19220 at 299.400 kb on - strand, within ABZR86_RS19220 at 299.495 kb on + strand at 299.534 kb on + strand at 299.534 kb on + strand at 299.534 kb on + strand at 299.535 kb on - strand at 299.535 kb on - strand at 299.535 kb on - strand at 299.709 kb on + strand, within ABZR86_RS19225 at 299.773 kb on - strand, within ABZR86_RS19225 at 299.773 kb on - strand, within ABZR86_RS19225 at 299.776 kb on - strand, within ABZR86_RS19225 at 299.955 kb on + strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 300.103 kb on - strand, within ABZR86_RS19225 at 300.363 kb on + strand at 300.364 kb on - strand at 300.364 kb on - strand at 300.364 kb on - strand at 300.365 kb on + strand at 300.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 298,001 + +0.9 298,001 + +0.3 298,053 + -0.4 298,054 - -0.1 298,070 + +1.2 298,116 + +0.0 298,116 + -0.1 298,157 + -0.1 298,158 - +0.9 298,158 - +0.1 298,271 + ABZR86_RS19215 0.15 +0.2 298,271 + ABZR86_RS19215 0.15 -0.2 298,271 + ABZR86_RS19215 0.15 -0.3 298,272 - ABZR86_RS19215 0.16 -0.6 298,272 - ABZR86_RS19215 0.16 +0.3 298,272 - ABZR86_RS19215 0.16 -0.1 298,307 + ABZR86_RS19215 0.20 +1.3 298,308 - ABZR86_RS19215 0.20 -0.7 298,308 - ABZR86_RS19215 0.20 +0.4 298,308 - ABZR86_RS19215 0.20 -0.2 298,355 + ABZR86_RS19215 0.27 +0.2 298,356 - ABZR86_RS19215 0.27 +0.4 298,356 - ABZR86_RS19215 0.27 +0.2 298,604 + ABZR86_RS19215 0.60 -0.4 298,604 + ABZR86_RS19215 0.60 -0.0 298,605 - ABZR86_RS19215 0.60 -0.1 298,785 + ABZR86_RS19215 0.84 -0.2 298,785 + ABZR86_RS19215 0.84 +0.7 298,836 - +0.1 298,836 - +0.1 298,836 - -0.8 298,908 + +0.2 298,909 - +0.3 299,255 + ABZR86_RS19220 0.58 +0.2 299,255 + ABZR86_RS19220 0.58 +1.3 299,256 - ABZR86_RS19220 0.58 +0.3 299,289 - ABZR86_RS19220 0.64 -0.0 299,357 + ABZR86_RS19220 0.78 -0.2 299,358 - ABZR86_RS19220 0.78 +0.1 299,358 - ABZR86_RS19220 0.78 +0.3 299,358 - ABZR86_RS19220 0.78 +0.5 299,399 + ABZR86_RS19220 0.86 -1.6 299,400 - ABZR86_RS19220 0.86 -0.1 299,495 + -0.5 299,534 + -0.5 299,534 + -0.1 299,534 + -0.9 299,535 - +0.1 299,535 - +0.4 299,535 - +1.0 299,709 + ABZR86_RS19225 0.21 -1.1 299,773 - ABZR86_RS19225 0.28 +0.0 299,773 - ABZR86_RS19225 0.28 -1.5 299,776 - ABZR86_RS19225 0.29 -0.7 299,955 + ABZR86_RS19225 0.48 -0.0 299,956 - ABZR86_RS19225 0.48 -2.4 299,956 - ABZR86_RS19225 0.48 -0.7 299,956 - ABZR86_RS19225 0.48 -1.7 300,103 - ABZR86_RS19225 0.65 +0.8 300,363 + -0.9 300,364 - +0.4 300,364 - -0.8 300,364 - -0.1 300,365 + -0.6 300,366 - -0.2
Or see this region's nucleotide sequence