Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS02535 and ABZR86_RS02540 overlap by 4 nucleotides ABZR86_RS02540 and ABZR86_RS02545 overlap by 4 nucleotides ABZR86_RS02545 and ABZR86_RS02550 overlap by 4 nucleotides ABZR86_RS02550 and ABZR86_RS02555 are separated by 215 nucleotides ABZR86_RS02555 and ABZR86_RS02560 overlap by 4 nucleotides
ABZR86_RS02535: ABZR86_RS02535 - hypothetical protein, at 518,792 to 519,040
_RS02535
ABZR86_RS02540: ABZR86_RS02540 - hypothetical protein, at 519,037 to 519,300
_RS02540
ABZR86_RS02545: ABZR86_RS02545 - Ref family recombination enhancement nuclease, at 519,297 to 519,848
_RS02545
ABZR86_RS02550: ABZR86_RS02550 - hypothetical protein, at 519,845 to 520,516
_RS02550
ABZR86_RS02555: ABZR86_RS02555 - hypothetical protein, at 520,732 to 520,923
_RS02555
ABZR86_RS02560: ABZR86_RS02560 - lysozyme, at 520,920 to 521,390
_RS02560
Position (kb)
519
520
521 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 518.982 kb on + strand, within ABZR86_RS02535 at 518.982 kb on + strand, within ABZR86_RS02535 at 518.982 kb on + strand, within ABZR86_RS02535 at 518.982 kb on + strand, within ABZR86_RS02535 at 518.982 kb on + strand, within ABZR86_RS02535 at 518.983 kb on - strand, within ABZR86_RS02535 at 519.101 kb on + strand, within ABZR86_RS02540 at 519.139 kb on + strand, within ABZR86_RS02540 at 519.139 kb on + strand, within ABZR86_RS02540 at 519.139 kb on + strand, within ABZR86_RS02540 at 519.139 kb on + strand, within ABZR86_RS02540 at 519.139 kb on + strand, within ABZR86_RS02540 at 519.140 kb on - strand, within ABZR86_RS02540 at 519.140 kb on - strand, within ABZR86_RS02540 at 519.140 kb on - strand, within ABZR86_RS02540 at 519.140 kb on - strand, within ABZR86_RS02540 at 519.150 kb on + strand, within ABZR86_RS02540 at 519.151 kb on - strand, within ABZR86_RS02540 at 519.253 kb on + strand, within ABZR86_RS02540 at 519.254 kb on - strand, within ABZR86_RS02540 at 519.254 kb on - strand, within ABZR86_RS02540 at 519.254 kb on - strand, within ABZR86_RS02540 at 519.254 kb on - strand, within ABZR86_RS02540 at 519.254 kb on - strand, within ABZR86_RS02540 at 519.366 kb on - strand, within ABZR86_RS02545 at 519.509 kb on + strand, within ABZR86_RS02545 at 519.558 kb on + strand, within ABZR86_RS02545 at 519.705 kb on + strand, within ABZR86_RS02545 at 519.706 kb on - strand, within ABZR86_RS02545 at 519.717 kb on + strand, within ABZR86_RS02545 at 519.717 kb on + strand, within ABZR86_RS02545 at 519.718 kb on - strand, within ABZR86_RS02545 at 519.750 kb on + strand, within ABZR86_RS02545 at 519.750 kb on + strand, within ABZR86_RS02545 at 519.750 kb on + strand, within ABZR86_RS02545 at 519.750 kb on + strand, within ABZR86_RS02545 at 519.751 kb on - strand, within ABZR86_RS02545 at 519.751 kb on - strand, within ABZR86_RS02545 at 519.778 kb on - strand, within ABZR86_RS02545 at 519.791 kb on + strand, within ABZR86_RS02545 at 519.791 kb on + strand, within ABZR86_RS02545 at 519.792 kb on - strand, within ABZR86_RS02545 at 519.793 kb on + strand at 519.794 kb on - strand at 519.794 kb on - strand at 519.794 kb on - strand at 519.894 kb on - strand at 519.894 kb on - strand at 520.094 kb on + strand, within ABZR86_RS02550 at 520.094 kb on + strand, within ABZR86_RS02550 at 520.094 kb on + strand, within ABZR86_RS02550 at 520.095 kb on - strand, within ABZR86_RS02550 at 520.095 kb on - strand, within ABZR86_RS02550 at 520.161 kb on - strand, within ABZR86_RS02550 at 520.174 kb on + strand, within ABZR86_RS02550 at 520.233 kb on - strand, within ABZR86_RS02550 at 520.233 kb on - strand, within ABZR86_RS02550 at 520.233 kb on - strand, within ABZR86_RS02550 at 520.233 kb on - strand, within ABZR86_RS02550 at 520.254 kb on + strand, within ABZR86_RS02550 at 520.255 kb on - strand, within ABZR86_RS02550 at 520.442 kb on + strand, within ABZR86_RS02550 at 520.442 kb on + strand, within ABZR86_RS02550 at 520.443 kb on - strand, within ABZR86_RS02550 at 520.443 kb on - strand, within ABZR86_RS02550 at 520.514 kb on + strand at 520.534 kb on + strand at 520.534 kb on + strand at 520.540 kb on + strand at 520.540 kb on + strand at 520.540 kb on + strand at 520.541 kb on - strand at 520.548 kb on + strand at 520.548 kb on + strand at 520.659 kb on + strand at 520.659 kb on + strand at 520.659 kb on + strand at 520.667 kb on + strand at 520.710 kb on + strand at 520.711 kb on - strand at 520.843 kb on + strand, within ABZR86_RS02555 at 520.843 kb on + strand, within ABZR86_RS02555 at 520.844 kb on - strand, within ABZR86_RS02555 at 520.844 kb on - strand, within ABZR86_RS02555 at 520.844 kb on - strand, within ABZR86_RS02555 at 520.942 kb on + strand at 520.942 kb on + strand at 520.943 kb on - strand at 521.091 kb on + strand, within ABZR86_RS02560 at 521.092 kb on - strand, within ABZR86_RS02560 at 521.116 kb on + strand, within ABZR86_RS02560 at 521.116 kb on + strand, within ABZR86_RS02560 at 521.117 kb on - strand, within ABZR86_RS02560 at 521.117 kb on - strand, within ABZR86_RS02560 at 521.313 kb on + strand, within ABZR86_RS02560 at 521.313 kb on + strand, within ABZR86_RS02560 at 521.372 kb on - strand at 521.438 kb on + strand at 521.439 kb on - strand at 521.439 kb on - strand at 521.478 kb on + strand at 521.478 kb on + strand at 521.479 kb on - strand at 521.479 kb on - strand at 521.494 kb on + strand at 521.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 518,982 + ABZR86_RS02535 0.76 -0.9 518,982 + ABZR86_RS02535 0.76 -0.3 518,982 + ABZR86_RS02535 0.76 -0.4 518,982 + ABZR86_RS02535 0.76 -0.1 518,982 + ABZR86_RS02535 0.76 +0.2 518,983 - ABZR86_RS02535 0.77 +0.1 519,101 + ABZR86_RS02540 0.24 -1.1 519,139 + ABZR86_RS02540 0.39 -0.1 519,139 + ABZR86_RS02540 0.39 +0.4 519,139 + ABZR86_RS02540 0.39 +0.3 519,139 + ABZR86_RS02540 0.39 +0.4 519,139 + ABZR86_RS02540 0.39 +0.0 519,140 - ABZR86_RS02540 0.39 +0.2 519,140 - ABZR86_RS02540 0.39 +1.3 519,140 - ABZR86_RS02540 0.39 +0.8 519,140 - ABZR86_RS02540 0.39 +0.5 519,150 + ABZR86_RS02540 0.43 +0.2 519,151 - ABZR86_RS02540 0.43 +0.3 519,253 + ABZR86_RS02540 0.82 +0.3 519,254 - ABZR86_RS02540 0.82 +0.5 519,254 - ABZR86_RS02540 0.82 +0.6 519,254 - ABZR86_RS02540 0.82 +0.4 519,254 - ABZR86_RS02540 0.82 +0.3 519,254 - ABZR86_RS02540 0.82 -0.6 519,366 - ABZR86_RS02545 0.12 -0.2 519,509 + ABZR86_RS02545 0.38 -0.1 519,558 + ABZR86_RS02545 0.47 +0.1 519,705 + ABZR86_RS02545 0.74 -4.1 519,706 - ABZR86_RS02545 0.74 +0.0 519,717 + ABZR86_RS02545 0.76 -1.8 519,717 + ABZR86_RS02545 0.76 -3.7 519,718 - ABZR86_RS02545 0.76 -0.5 519,750 + ABZR86_RS02545 0.82 -2.9 519,750 + ABZR86_RS02545 0.82 -3.6 519,750 + ABZR86_RS02545 0.82 -2.5 519,750 + ABZR86_RS02545 0.82 -3.8 519,751 - ABZR86_RS02545 0.82 +0.1 519,751 - ABZR86_RS02545 0.82 +0.3 519,778 - ABZR86_RS02545 0.87 +0.0 519,791 + ABZR86_RS02545 0.89 -1.1 519,791 + ABZR86_RS02545 0.89 -0.8 519,792 - ABZR86_RS02545 0.90 +0.0 519,793 + -1.5 519,794 - +0.3 519,794 - +0.6 519,794 - +0.2 519,894 - +0.3 519,894 - -0.5 520,094 + ABZR86_RS02550 0.37 +0.6 520,094 + ABZR86_RS02550 0.37 +0.4 520,094 + ABZR86_RS02550 0.37 +0.0 520,095 - ABZR86_RS02550 0.37 +0.7 520,095 - ABZR86_RS02550 0.37 +0.3 520,161 - ABZR86_RS02550 0.47 -0.1 520,174 + ABZR86_RS02550 0.49 -0.6 520,233 - ABZR86_RS02550 0.58 -0.4 520,233 - ABZR86_RS02550 0.58 -0.3 520,233 - ABZR86_RS02550 0.58 +0.2 520,233 - ABZR86_RS02550 0.58 -0.9 520,254 + ABZR86_RS02550 0.61 +1.2 520,255 - ABZR86_RS02550 0.61 +1.4 520,442 + ABZR86_RS02550 0.89 -0.2 520,442 + ABZR86_RS02550 0.89 -0.1 520,443 - ABZR86_RS02550 0.89 +0.1 520,443 - ABZR86_RS02550 0.89 -0.3 520,514 + +0.0 520,534 + -0.3 520,534 + -0.2 520,540 + -0.4 520,540 + +0.3 520,540 + -0.4 520,541 - -0.4 520,548 + +1.4 520,548 + -0.2 520,659 + -1.3 520,659 + +0.6 520,659 + -0.5 520,667 + -1.9 520,710 + -0.1 520,711 - +0.4 520,843 + ABZR86_RS02555 0.58 +0.1 520,843 + ABZR86_RS02555 0.58 -0.1 520,844 - ABZR86_RS02555 0.58 +0.4 520,844 - ABZR86_RS02555 0.58 -0.1 520,844 - ABZR86_RS02555 0.58 +0.1 520,942 + +0.0 520,942 + +0.4 520,943 - +0.5 521,091 + ABZR86_RS02560 0.36 +0.1 521,092 - ABZR86_RS02560 0.37 +0.2 521,116 + ABZR86_RS02560 0.42 +0.1 521,116 + ABZR86_RS02560 0.42 +0.2 521,117 - ABZR86_RS02560 0.42 +0.0 521,117 - ABZR86_RS02560 0.42 -0.3 521,313 + ABZR86_RS02560 0.83 -0.4 521,313 + ABZR86_RS02560 0.83 -0.1 521,372 - -1.0 521,438 + +0.3 521,439 - -0.1 521,439 - -0.2 521,478 + -1.0 521,478 + -0.6 521,479 - +0.4 521,479 - +0.7 521,494 + +0.6 521,495 - -0.4
Or see this region's nucleotide sequence