Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3335 and Shewana3_3336 are separated by 400 nucleotides Shewana3_3336 and Shewana3_3337 are separated by 267 nucleotides
Shewana3_3335: Shewana3_3335 - multi-sensor hybrid histidine kinase (RefSeq), at 3,997,279 to 4,000,647
_3335
Shewana3_3336: Shewana3_3336 - ATP-dependent RNA helicase SrmB (RefSeq), at 4,001,048 to 4,002,307
_3336
Shewana3_3337: Shewana3_3337 - RND family efflux transporter MFP subunit (RefSeq), at 4,002,575 to 4,003,792
_3337
Position (kb)
4001
4002
4003 Strain fitness (log2 ratio)
-2
-1
0
1 at 4000.100 kb on - strand, within Shewana3_3335 at 4000.203 kb on + strand, within Shewana3_3335 at 4000.236 kb on + strand, within Shewana3_3335 at 4000.301 kb on - strand, within Shewana3_3335 at 4000.338 kb on + strand at 4000.368 kb on - strand at 4000.436 kb on - strand at 4000.477 kb on + strand at 4000.485 kb on - strand at 4000.485 kb on - strand at 4000.485 kb on - strand at 4000.702 kb on + strand at 4000.717 kb on - strand at 4001.008 kb on + strand at 4001.380 kb on + strand, within Shewana3_3336 at 4001.380 kb on + strand, within Shewana3_3336 at 4001.570 kb on + strand, within Shewana3_3336 at 4001.578 kb on - strand, within Shewana3_3336 at 4001.703 kb on - strand, within Shewana3_3336 at 4001.765 kb on - strand, within Shewana3_3336 at 4001.850 kb on - strand, within Shewana3_3336 at 4002.030 kb on + strand, within Shewana3_3336 at 4002.170 kb on + strand, within Shewana3_3336 at 4002.269 kb on + strand at 4002.302 kb on + strand at 4002.372 kb on + strand at 4002.393 kb on + strand at 4002.456 kb on + strand at 4002.815 kb on - strand, within Shewana3_3337 at 4003.099 kb on + strand, within Shewana3_3337 at 4003.223 kb on + strand, within Shewana3_3337 at 4003.263 kb on + strand, within Shewana3_3337
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 4,000,100 - Shewana3_3335 0.84 -0.2 4,000,203 + Shewana3_3335 0.87 -1.5 4,000,236 + Shewana3_3335 0.88 +0.4 4,000,301 - Shewana3_3335 0.90 -0.6 4,000,338 + -0.8 4,000,368 - -0.6 4,000,436 - -0.5 4,000,477 + -0.1 4,000,485 - +0.2 4,000,485 - -0.1 4,000,485 - +0.1 4,000,702 + -1.5 4,000,717 - +0.0 4,001,008 + -2.1 4,001,380 + Shewana3_3336 0.26 -0.4 4,001,380 + Shewana3_3336 0.26 -0.9 4,001,570 + Shewana3_3336 0.41 -1.5 4,001,578 - Shewana3_3336 0.42 -0.6 4,001,703 - Shewana3_3336 0.52 -2.6 4,001,765 - Shewana3_3336 0.57 -0.5 4,001,850 - Shewana3_3336 0.64 -0.3 4,002,030 + Shewana3_3336 0.78 -1.2 4,002,170 + Shewana3_3336 0.89 -0.7 4,002,269 + -1.9 4,002,302 + -0.5 4,002,372 + -1.1 4,002,393 + -1.5 4,002,456 + +0.9 4,002,815 - Shewana3_3337 0.20 -0.5 4,003,099 + Shewana3_3337 0.43 +0.1 4,003,223 + Shewana3_3337 0.53 -0.9 4,003,263 + Shewana3_3337 0.56 +0.5
Or see this region's nucleotide sequence