Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acpS and Shewana3_3023 overlap by 1 nucleotides Shewana3_3023 and Shewana3_3024 are separated by 89 nucleotides Shewana3_3024 and era are separated by 49 nucleotides
Shewana3_3022: acpS - 4'-phosphopantetheinyl transferase (RefSeq), at 3,628,451 to 3,628,834
acpS
Shewana3_3023: Shewana3_3023 - pyridoxine 5'-phosphate synthase (RefSeq), at 3,628,834 to 3,629,571
_3023
Shewana3_3024: Shewana3_3024 - DNA repair protein RecO (RefSeq), at 3,629,661 to 3,630,362
_3024
Shewana3_3025: era - GTP-binding protein Era (RefSeq), at 3,630,412 to 3,631,431
era
Position (kb)
3629
3630
3631 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3628.778 kb on - strand, within acpS at 3629.629 kb on - strand at 3629.629 kb on - strand at 3629.840 kb on + strand, within Shewana3_3024 at 3629.975 kb on + strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.990 kb on + strand, within Shewana3_3024 at 3630.066 kb on - strand, within Shewana3_3024 at 3630.187 kb on - strand, within Shewana3_3024 at 3630.328 kb on - strand at 3630.409 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 3,628,778 - acpS Shewana3_3022 0.85 +0.6 3,629,629 - +1.2 3,629,629 - -1.7 3,629,840 + Shewana3_3024 0.25 -0.4 3,629,975 + Shewana3_3024 0.45 -4.3 3,629,983 - Shewana3_3024 0.46 -2.9 3,629,983 - Shewana3_3024 0.46 -2.3 3,629,990 + Shewana3_3024 0.47 -2.2 3,630,066 - Shewana3_3024 0.58 -2.7 3,630,187 - Shewana3_3024 0.75 -3.6 3,630,328 - -2.7 3,630,409 + -2.1
Or see this region's nucleotide sequence