Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2158 and Shewana3_2159 are separated by 27 nucleotides Shewana3_2159 and Shewana3_2160 are separated by 79 nucleotides Shewana3_2160 and Shewana3_2161 are separated by 188 nucleotides Shewana3_2161 and Shewana3_2162 are separated by 249 nucleotides
Shewana3_2158: Shewana3_2158 - hypothetical protein (RefSeq), at 2,586,807 to 2,587,385
_2158
Shewana3_2159: Shewana3_2159 - SecC motif-containing protein (RefSeq), at 2,587,413 to 2,587,907
_2159
Shewana3_2160: Shewana3_2160 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,587,987 to 2,590,170
_2160
Shewana3_2161: Shewana3_2161 - hypothetical protein (RefSeq), at 2,590,359 to 2,590,646
_2161
Shewana3_2162: Shewana3_2162 - hypothetical protein (RefSeq), at 2,590,896 to 2,591,384
_2162
Position (kb)
2587
2588
2589
2590
2591 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2587.516 kb on + strand, within Shewana3_2159 at 2587.615 kb on + strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.635 kb on + strand, within Shewana3_2159 at 2587.651 kb on + strand, within Shewana3_2159 at 2587.681 kb on + strand, within Shewana3_2159 at 2587.895 kb on + strand at 2588.416 kb on - strand, within Shewana3_2160 at 2588.416 kb on - strand, within Shewana3_2160 at 2588.798 kb on + strand, within Shewana3_2160 at 2589.153 kb on - strand, within Shewana3_2160 at 2589.167 kb on - strand, within Shewana3_2160 at 2589.374 kb on - strand, within Shewana3_2160 at 2589.419 kb on + strand, within Shewana3_2160 at 2589.612 kb on - strand, within Shewana3_2160 at 2589.841 kb on - strand at 2589.868 kb on - strand, within Shewana3_2160 at 2589.946 kb on - strand, within Shewana3_2160 at 2590.174 kb on + strand at 2590.350 kb on + strand at 2590.358 kb on - strand at 2590.515 kb on - strand, within Shewana3_2161 at 2590.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,587,516 + Shewana3_2159 0.21 +0.8 2,587,615 + Shewana3_2159 0.41 +1.8 2,587,623 - Shewana3_2159 0.42 +1.8 2,587,623 - Shewana3_2159 0.42 +0.8 2,587,635 + Shewana3_2159 0.45 +0.7 2,587,651 + Shewana3_2159 0.48 -0.5 2,587,681 + Shewana3_2159 0.54 +0.6 2,587,895 + -0.3 2,588,416 - Shewana3_2160 0.20 -0.5 2,588,416 - Shewana3_2160 0.20 +0.8 2,588,798 + Shewana3_2160 0.37 +1.8 2,589,153 - Shewana3_2160 0.53 +0.8 2,589,167 - Shewana3_2160 0.54 -1.5 2,589,374 - Shewana3_2160 0.64 +0.8 2,589,419 + Shewana3_2160 0.66 +1.8 2,589,612 - Shewana3_2160 0.74 -1.7 2,589,841 - +1.8 2,589,868 - Shewana3_2160 0.86 +0.2 2,589,946 - Shewana3_2160 0.90 +1.8 2,590,174 + +0.2 2,590,350 + -1.7 2,590,358 - -2.7 2,590,515 - Shewana3_2161 0.54 +0.2 2,590,675 - +1.8
Or see this region's nucleotide sequence