Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shewana3_1942 are separated by 111 nucleotides Shewana3_1942 and Shewana3_1943 are separated by 2 nucleotides Shewana3_1943 and Shewana3_1944 are separated by 174 nucleotides
Shewana3_1941: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,307,669 to 2,308,784
thiH
Shewana3_1942: Shewana3_1942 - methyl-accepting chemotaxis protein (RefSeq), at 2,308,896 to 2,309,159
_1942
Shewana3_1943: Shewana3_1943 - protein-glutamate O-methyltransferase (RefSeq), at 2,309,162 to 2,311,384
_1943
Shewana3_1944: Shewana3_1944 - LysR family transcriptional regulator (RefSeq), at 2,311,559 to 2,312,461
_1944
Position (kb)
2309
2310
2311
2312 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2308.335 kb on - strand, within thiH at 2308.389 kb on - strand, within thiH at 2308.459 kb on - strand, within thiH at 2308.473 kb on - strand, within thiH at 2308.512 kb on + strand, within thiH at 2308.596 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.625 kb on - strand, within thiH at 2308.645 kb on + strand, within thiH at 2308.709 kb on + strand at 2308.987 kb on - strand, within Shewana3_1942 at 2309.379 kb on + strand at 2309.615 kb on + strand, within Shewana3_1943 at 2309.852 kb on + strand, within Shewana3_1943 at 2310.077 kb on - strand, within Shewana3_1943 at 2310.228 kb on + strand, within Shewana3_1943 at 2310.482 kb on + strand, within Shewana3_1943 at 2310.521 kb on + strand, within Shewana3_1943 at 2310.794 kb on - strand, within Shewana3_1943 at 2310.901 kb on + strand, within Shewana3_1943 at 2311.204 kb on + strand at 2311.349 kb on - strand at 2311.412 kb on - strand at 2311.425 kb on + strand at 2311.894 kb on + strand, within Shewana3_1944 at 2312.308 kb on + strand, within Shewana3_1944 at 2312.360 kb on - strand, within Shewana3_1944
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,308,335 - thiH Shewana3_1941 0.60 +0.2 2,308,389 - thiH Shewana3_1941 0.65 -1.6 2,308,459 - thiH Shewana3_1941 0.71 +0.8 2,308,473 - thiH Shewana3_1941 0.72 +1.8 2,308,512 + thiH Shewana3_1941 0.76 -0.0 2,308,596 - thiH Shewana3_1941 0.83 +0.8 2,308,616 - thiH Shewana3_1941 0.85 +0.2 2,308,616 - thiH Shewana3_1941 0.85 +0.2 2,308,616 - thiH Shewana3_1941 0.85 -0.2 2,308,625 - thiH Shewana3_1941 0.86 +0.8 2,308,645 + thiH Shewana3_1941 0.87 -2.1 2,308,709 + +1.5 2,308,987 - Shewana3_1942 0.34 -0.5 2,309,379 + +1.8 2,309,615 + Shewana3_1943 0.20 -1.6 2,309,852 + Shewana3_1943 0.31 +1.2 2,310,077 - Shewana3_1943 0.41 +1.5 2,310,228 + Shewana3_1943 0.48 +1.2 2,310,482 + Shewana3_1943 0.59 -1.5 2,310,521 + Shewana3_1943 0.61 -1.7 2,310,794 - Shewana3_1943 0.73 +1.8 2,310,901 + Shewana3_1943 0.78 +1.3 2,311,204 + +0.2 2,311,349 - +1.8 2,311,412 - -0.8 2,311,425 + -0.5 2,311,894 + Shewana3_1944 0.37 +0.5 2,312,308 + Shewana3_1944 0.83 +1.2 2,312,360 - Shewana3_1944 0.89 -0.5
Or see this region's nucleotide sequence