Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1938 and Shewana3_1939 overlap by 4 nucleotides Shewana3_1939 and thiG are separated by 1 nucleotides thiG and thiH are separated by 2 nucleotides
Shewana3_1938: Shewana3_1938 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,305,690 to 2,306,706
_1938
Shewana3_1939: Shewana3_1939 - thiamine biosynthesis protein ThiS (RefSeq), at 2,306,703 to 2,306,906
_1939
Shewana3_1940: thiG - thiazole synthase (RefSeq), at 2,306,908 to 2,307,666
thiG
Shewana3_1941: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,307,669 to 2,308,784
thiH
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2305.960 kb on - strand, within Shewana3_1938 at 2306.164 kb on - strand, within Shewana3_1938 at 2306.166 kb on + strand, within Shewana3_1938 at 2306.350 kb on - strand, within Shewana3_1938 at 2306.457 kb on - strand, within Shewana3_1938 at 2306.803 kb on + strand, within Shewana3_1939 at 2306.845 kb on + strand, within Shewana3_1939 at 2306.853 kb on - strand, within Shewana3_1939 at 2306.993 kb on + strand, within thiG at 2307.113 kb on + strand, within thiG at 2307.253 kb on + strand, within thiG at 2307.563 kb on + strand, within thiG at 2307.634 kb on + strand at 2307.634 kb on + strand at 2307.634 kb on + strand at 2307.642 kb on - strand at 2307.765 kb on - strand at 2307.927 kb on - strand, within thiH at 2308.149 kb on + strand, within thiH at 2308.335 kb on - strand, within thiH at 2308.389 kb on - strand, within thiH at 2308.459 kb on - strand, within thiH at 2308.473 kb on - strand, within thiH at 2308.512 kb on + strand, within thiH at 2308.596 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.625 kb on - strand, within thiH at 2308.645 kb on + strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 2,305,960 - Shewana3_1938 0.27 +0.2 2,306,164 - Shewana3_1938 0.47 +0.2 2,306,166 + Shewana3_1938 0.47 +0.2 2,306,350 - Shewana3_1938 0.65 -1.2 2,306,457 - Shewana3_1938 0.75 +0.2 2,306,803 + Shewana3_1939 0.49 -1.0 2,306,845 + Shewana3_1939 0.70 -0.0 2,306,853 - Shewana3_1939 0.74 -0.2 2,306,993 + thiG Shewana3_1940 0.11 -0.0 2,307,113 + thiG Shewana3_1940 0.27 +1.2 2,307,253 + thiG Shewana3_1940 0.45 +1.8 2,307,563 + thiG Shewana3_1940 0.86 +0.5 2,307,634 + +2.6 2,307,634 + +0.3 2,307,634 + +1.2 2,307,642 - +0.8 2,307,765 - -0.2 2,307,927 - thiH Shewana3_1941 0.23 +1.1 2,308,149 + thiH Shewana3_1941 0.43 -1.6 2,308,335 - thiH Shewana3_1941 0.60 +0.2 2,308,389 - thiH Shewana3_1941 0.65 -1.6 2,308,459 - thiH Shewana3_1941 0.71 +0.8 2,308,473 - thiH Shewana3_1941 0.72 +1.8 2,308,512 + thiH Shewana3_1941 0.76 -0.0 2,308,596 - thiH Shewana3_1941 0.83 +0.8 2,308,616 - thiH Shewana3_1941 0.85 +0.2 2,308,616 - thiH Shewana3_1941 0.85 +0.2 2,308,616 - thiH Shewana3_1941 0.85 -0.2 2,308,625 - thiH Shewana3_1941 0.86 +0.8 2,308,645 + thiH Shewana3_1941 0.87 -2.1
Or see this region's nucleotide sequence