Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0072 and Shewana3_R0073 are separated by 175 nucleotides Shewana3_R0073 and Shewana3_1584 are separated by 259 nucleotides Shewana3_1584 and cpdB are separated by 226 nucleotides
Shewana3_R0072: Shewana3_R0072 - 23S ribosomal RNA (RefSeq), at 1,855,447 to 1,858,341
_R0072
Shewana3_R0073: Shewana3_R0073 - 5S ribosomal RNA (RefSeq), at 1,858,517 to 1,858,636
_R0073
Shewana3_1584: Shewana3_1584 - hypothetical protein (RefSeq), at 1,858,896 to 1,859,612
_1584
Shewana3_1585: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 1,859,839 to 1,861,896
cpdB
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-2
-1
0
1 at 1858.848 kb on + strand at 1859.008 kb on + strand, within Shewana3_1584 at 1859.037 kb on - strand, within Shewana3_1584 at 1859.054 kb on - strand, within Shewana3_1584 at 1859.087 kb on + strand, within Shewana3_1584 at 1859.281 kb on + strand, within Shewana3_1584 at 1859.672 kb on + strand at 1859.711 kb on - strand at 1860.223 kb on + strand, within cpdB at 1860.233 kb on + strand at 1860.240 kb on - strand, within cpdB at 1860.265 kb on - strand, within cpdB at 1860.419 kb on + strand, within cpdB at 1860.557 kb on + strand, within cpdB
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,858,848 + -0.6 1,859,008 + Shewana3_1584 0.16 -1.5 1,859,037 - Shewana3_1584 0.20 -0.3 1,859,054 - Shewana3_1584 0.22 +0.1 1,859,087 + Shewana3_1584 0.27 -0.3 1,859,281 + Shewana3_1584 0.54 -2.1 1,859,672 + +1.1 1,859,711 - +1.7 1,860,223 + cpdB Shewana3_1585 0.19 +1.7 1,860,233 + +1.7 1,860,240 - cpdB Shewana3_1585 0.19 +0.4 1,860,265 - cpdB Shewana3_1585 0.21 +0.1 1,860,419 + cpdB Shewana3_1585 0.28 +1.7 1,860,557 + cpdB Shewana3_1585 0.35 +1.7
Or see this region's nucleotide sequence