Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1552 and Shewana3_1553 are separated by 87 nucleotides Shewana3_1553 and pflA are separated by 15 nucleotides pflA and Shewana3_1555 are separated by 63 nucleotides
Shewana3_1552: Shewana3_1552 - acetate kinase (RefSeq), at 1,808,697 to 1,809,896
_1552
Shewana3_1553: Shewana3_1553 - hypothetical protein (RefSeq), at 1,809,984 to 1,810,481
_1553
Shewana3_1554: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 1,810,497 to 1,811,237
pflA
Shewana3_1555: Shewana3_1555 - formate acetyltransferase (RefSeq), at 1,811,301 to 1,813,583
_1555
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-1
0
1 at 1810.002 kb on - strand at 1810.009 kb on - strand at 1810.011 kb on + strand at 1810.213 kb on - strand, within Shewana3_1553 at 1810.266 kb on + strand, within Shewana3_1553 at 1810.475 kb on + strand at 1810.568 kb on - strand at 1810.797 kb on - strand, within pflA at 1810.865 kb on - strand, within pflA at 1810.965 kb on - strand, within pflA at 1811.141 kb on + strand, within pflA at 1811.156 kb on + strand, within pflA at 1811.318 kb on + strand at 1811.374 kb on + strand at 1811.382 kb on - strand at 1811.660 kb on + strand, within Shewana3_1555 at 1811.712 kb on - strand, within Shewana3_1555 at 1811.779 kb on - strand, within Shewana3_1555 at 1811.944 kb on + strand, within Shewana3_1555 at 1812.029 kb on + strand, within Shewana3_1555 at 1812.106 kb on + strand, within Shewana3_1555
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,810,002 - -0.3 1,810,009 - +1.7 1,810,011 + +1.7 1,810,213 - Shewana3_1553 0.46 +0.3 1,810,266 + Shewana3_1553 0.57 +0.7 1,810,475 + -0.9 1,810,568 - -0.8 1,810,797 - pflA Shewana3_1554 0.40 -1.3 1,810,865 - pflA Shewana3_1554 0.50 -0.5 1,810,965 - pflA Shewana3_1554 0.63 -1.1 1,811,141 + pflA Shewana3_1554 0.87 +0.4 1,811,156 + pflA Shewana3_1554 0.89 -1.1 1,811,318 + -0.9 1,811,374 + -0.3 1,811,382 - -1.1 1,811,660 + Shewana3_1555 0.16 +0.9 1,811,712 - Shewana3_1555 0.18 +0.3 1,811,779 - Shewana3_1555 0.21 +0.5 1,811,944 + Shewana3_1555 0.28 +0.1 1,812,029 + Shewana3_1555 0.32 +1.5 1,812,106 + Shewana3_1555 0.35 -0.9
Or see this region's nucleotide sequence