Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt surE and Shewana3_1122 overlap by 4 nucleotides Shewana3_1122 and Shewana3_1123 are separated by 84 nucleotides Shewana3_1123 and Shewana3_1124 are separated by 80 nucleotides
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Shewana3_1123: Shewana3_1123 - peptidase M23B (RefSeq), at 1,321,357 to 1,322,253
_1123
Shewana3_1124: Shewana3_1124 - RNA polymerase, sigma 38 subunit, RpoS (RefSeq), at 1,322,334 to 1,323,314
_1124
Position (kb)
1321
1322
1323 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122 at 1320.950 kb on + strand, within Shewana3_1122 at 1321.345 kb on - strand at 1321.439 kb on - strand at 1321.544 kb on - strand, within Shewana3_1123 at 1321.878 kb on - strand, within Shewana3_1123 at 1321.922 kb on + strand, within Shewana3_1123 at 1322.101 kb on + strand, within Shewana3_1123 at 1322.243 kb on - strand at 1322.568 kb on + strand, within Shewana3_1124 at 1322.958 kb on - strand, within Shewana3_1124 at 1323.025 kb on - strand, within Shewana3_1124 at 1323.151 kb on + strand, within Shewana3_1124
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,320,397 + surE Shewana3_1121 0.67 -3.3 1,320,433 + surE Shewana3_1121 0.72 -3.5 1,320,441 - surE Shewana3_1121 0.73 -3.4 1,320,578 + -2.7 1,320,728 + Shewana3_1122 0.14 -0.7 1,320,950 + Shewana3_1122 0.49 -1.1 1,321,345 - -1.7 1,321,439 - -0.1 1,321,544 - Shewana3_1123 0.21 +1.5 1,321,878 - Shewana3_1123 0.58 -1.5 1,321,922 + Shewana3_1123 0.63 -1.1 1,322,101 + Shewana3_1123 0.83 -0.1 1,322,243 - +1.5 1,322,568 + Shewana3_1124 0.24 +0.0 1,322,958 - Shewana3_1124 0.64 -1.1 1,323,025 - Shewana3_1124 0.70 +0.2 1,323,151 + Shewana3_1124 0.83 +1.4
Or see this region's nucleotide sequence