Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_878 and Pf6N2E2_879 are separated by 238 nucleotides Pf6N2E2_879 and Pf6N2E2_880 are separated by 106 nucleotides Pf6N2E2_880 and Pf6N2E2_881 are separated by 87 nucleotides
Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539
_878
Pf6N2E2_879 - Cyanoglobin; Hemoglobin-like protein HbN, at 1,028,778 to 1,029,302
_879
Pf6N2E2_880 - QbdB, at 1,029,409 to 1,030,275
_880
Pf6N2E2_881 - Aldoxime dehydratase, at 1,030,363 to 1,031,418
_881
Position (kb)
1029
1030
1031 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1028.418 kb on + strand at 1028.452 kb on - strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.480 kb on + strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.481 kb on - strand at 1028.654 kb on - strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.661 kb on + strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.662 kb on - strand at 1028.759 kb on + strand at 1028.760 kb on - strand at 1028.760 kb on - strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.765 kb on + strand at 1028.766 kb on - strand at 1028.766 kb on - strand at 1028.992 kb on + strand, within Pf6N2E2_879 at 1028.993 kb on - strand, within Pf6N2E2_879 at 1029.082 kb on + strand, within Pf6N2E2_879 at 1029.083 kb on - strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.161 kb on + strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.162 kb on - strand, within Pf6N2E2_879 at 1029.210 kb on + strand, within Pf6N2E2_879 at 1029.229 kb on + strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.230 kb on - strand, within Pf6N2E2_879 at 1029.242 kb on - strand, within Pf6N2E2_879 at 1029.258 kb on - strand at 1029.259 kb on + strand at 1029.259 kb on + strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.260 kb on - strand at 1029.379 kb on - strand at 1029.395 kb on - strand at 1029.411 kb on - strand at 1029.426 kb on - strand at 1029.647 kb on + strand, within Pf6N2E2_880 at 1029.658 kb on + strand, within Pf6N2E2_880 at 1029.672 kb on - strand, within Pf6N2E2_880 at 1029.786 kb on - strand, within Pf6N2E2_880 at 1029.786 kb on - strand, within Pf6N2E2_880 at 1029.786 kb on - strand, within Pf6N2E2_880 at 1029.849 kb on - strand, within Pf6N2E2_880 at 1029.866 kb on + strand, within Pf6N2E2_880 at 1029.866 kb on + strand, within Pf6N2E2_880 at 1029.867 kb on - strand, within Pf6N2E2_880 at 1029.872 kb on + strand, within Pf6N2E2_880 at 1029.873 kb on - strand, within Pf6N2E2_880 at 1030.049 kb on + strand, within Pf6N2E2_880 at 1030.049 kb on + strand, within Pf6N2E2_880 at 1030.050 kb on - strand, within Pf6N2E2_880 at 1030.124 kb on + strand, within Pf6N2E2_880 at 1030.151 kb on + strand, within Pf6N2E2_880 at 1030.152 kb on - strand, within Pf6N2E2_880 at 1030.193 kb on + strand at 1030.193 kb on + strand at 1030.421 kb on + strand at 1030.422 kb on - strand at 1030.518 kb on + strand, within Pf6N2E2_881 at 1030.518 kb on + strand, within Pf6N2E2_881 at 1030.522 kb on + strand, within Pf6N2E2_881 at 1030.523 kb on - strand, within Pf6N2E2_881 at 1030.589 kb on + strand, within Pf6N2E2_881 at 1030.646 kb on + strand, within Pf6N2E2_881 at 1030.646 kb on + strand, within Pf6N2E2_881 at 1030.721 kb on + strand, within Pf6N2E2_881 at 1030.722 kb on - strand, within Pf6N2E2_881 at 1030.881 kb on + strand, within Pf6N2E2_881 at 1030.882 kb on - strand, within Pf6N2E2_881 at 1030.882 kb on - strand, within Pf6N2E2_881 at 1030.882 kb on - strand, within Pf6N2E2_881 at 1030.985 kb on + strand, within Pf6N2E2_881 at 1030.985 kb on + strand, within Pf6N2E2_881 at 1030.986 kb on - strand, within Pf6N2E2_881 at 1030.986 kb on - strand, within Pf6N2E2_881 at 1030.986 kb on - strand, within Pf6N2E2_881 at 1031.095 kb on + strand, within Pf6N2E2_881 at 1031.096 kb on - strand, within Pf6N2E2_881 at 1031.096 kb on - strand, within Pf6N2E2_881 at 1031.174 kb on + strand, within Pf6N2E2_881 at 1031.175 kb on - strand, within Pf6N2E2_881 at 1031.228 kb on + strand, within Pf6N2E2_881 at 1031.229 kb on - strand, within Pf6N2E2_881
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,028,418 + -0.8 1,028,452 - -0.3 1,028,480 + -2.5 1,028,480 + +0.7 1,028,480 + +0.7 1,028,480 + -0.4 1,028,480 + -0.0 1,028,480 + -0.0 1,028,480 + +0.0 1,028,481 - +0.5 1,028,481 - +1.4 1,028,481 - +1.1 1,028,481 - -0.2 1,028,654 - -0.6 1,028,661 + +0.7 1,028,661 + +0.2 1,028,661 + +0.4 1,028,662 - -0.3 1,028,662 - +0.2 1,028,662 - +0.4 1,028,759 + -0.8 1,028,760 - +0.5 1,028,760 - +2.6 1,028,765 + +0.2 1,028,765 + -1.9 1,028,765 + +0.4 1,028,766 - +0.2 1,028,766 - -0.1 1,028,992 + Pf6N2E2_879 0.41 +0.9 1,028,993 - Pf6N2E2_879 0.41 +1.3 1,029,082 + Pf6N2E2_879 0.58 +1.7 1,029,083 - Pf6N2E2_879 0.58 -1.0 1,029,161 + Pf6N2E2_879 0.73 +0.7 1,029,161 + Pf6N2E2_879 0.73 +1.0 1,029,161 + Pf6N2E2_879 0.73 +0.9 1,029,161 + Pf6N2E2_879 0.73 +0.4 1,029,162 - Pf6N2E2_879 0.73 +0.0 1,029,162 - Pf6N2E2_879 0.73 +1.0 1,029,162 - Pf6N2E2_879 0.73 +3.0 1,029,162 - Pf6N2E2_879 0.73 -0.3 1,029,210 + Pf6N2E2_879 0.82 -1.0 1,029,229 + Pf6N2E2_879 0.86 -0.2 1,029,230 - Pf6N2E2_879 0.86 +1.3 1,029,230 - Pf6N2E2_879 0.86 +0.4 1,029,230 - Pf6N2E2_879 0.86 +0.3 1,029,230 - Pf6N2E2_879 0.86 -0.2 1,029,230 - Pf6N2E2_879 0.86 +0.1 1,029,242 - Pf6N2E2_879 0.88 +1.3 1,029,258 - -0.3 1,029,259 + -1.1 1,029,259 + +1.3 1,029,260 - -0.6 1,029,260 - +1.1 1,029,260 - +0.2 1,029,260 - -2.3 1,029,379 - +1.2 1,029,395 - +1.6 1,029,411 - -2.7 1,029,426 - -0.1 1,029,647 + Pf6N2E2_880 0.27 -0.4 1,029,658 + Pf6N2E2_880 0.29 -1.1 1,029,672 - Pf6N2E2_880 0.30 +0.4 1,029,786 - Pf6N2E2_880 0.43 +0.4 1,029,786 - Pf6N2E2_880 0.43 +0.4 1,029,786 - Pf6N2E2_880 0.43 -1.3 1,029,849 - Pf6N2E2_880 0.51 -1.6 1,029,866 + Pf6N2E2_880 0.53 +1.2 1,029,866 + Pf6N2E2_880 0.53 -1.2 1,029,867 - Pf6N2E2_880 0.53 -0.2 1,029,872 + Pf6N2E2_880 0.53 -2.5 1,029,873 - Pf6N2E2_880 0.54 +2.5 1,030,049 + Pf6N2E2_880 0.74 -0.4 1,030,049 + Pf6N2E2_880 0.74 -0.8 1,030,050 - Pf6N2E2_880 0.74 -3.1 1,030,124 + Pf6N2E2_880 0.82 -0.7 1,030,151 + Pf6N2E2_880 0.86 -0.3 1,030,152 - Pf6N2E2_880 0.86 -2.6 1,030,193 + -2.1 1,030,193 + -0.8 1,030,421 + -0.4 1,030,422 - +0.7 1,030,518 + Pf6N2E2_881 0.15 -3.1 1,030,518 + Pf6N2E2_881 0.15 -2.0 1,030,522 + Pf6N2E2_881 0.15 +0.8 1,030,523 - Pf6N2E2_881 0.15 -1.8 1,030,589 + Pf6N2E2_881 0.21 -1.4 1,030,646 + Pf6N2E2_881 0.27 +2.0 1,030,646 + Pf6N2E2_881 0.27 -1.4 1,030,721 + Pf6N2E2_881 0.34 -0.6 1,030,722 - Pf6N2E2_881 0.34 -1.5 1,030,881 + Pf6N2E2_881 0.49 +1.0 1,030,882 - Pf6N2E2_881 0.49 +1.6 1,030,882 - Pf6N2E2_881 0.49 +0.5 1,030,882 - Pf6N2E2_881 0.49 -0.8 1,030,985 + Pf6N2E2_881 0.59 -0.1 1,030,985 + Pf6N2E2_881 0.59 -1.4 1,030,986 - Pf6N2E2_881 0.59 +1.1 1,030,986 - Pf6N2E2_881 0.59 -1.2 1,030,986 - Pf6N2E2_881 0.59 -0.6 1,031,095 + Pf6N2E2_881 0.69 +0.6 1,031,096 - Pf6N2E2_881 0.69 +2.1 1,031,096 - Pf6N2E2_881 0.69 +1.3 1,031,174 + Pf6N2E2_881 0.77 +0.6 1,031,175 - Pf6N2E2_881 0.77 -1.5 1,031,228 + Pf6N2E2_881 0.82 +1.2 1,031,229 - Pf6N2E2_881 0.82 +3.2
Or see this region's nucleotide sequence