Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_729 and Pf6N2E2_730 are separated by 1 nucleotides Pf6N2E2_730 and Pf6N2E2_731 are separated by 2 nucleotides Pf6N2E2_731 and Pf6N2E2_732 overlap by 8 nucleotides
Pf6N2E2_729 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 834,236 to 838,009
_729
Pf6N2E2_730 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 838,011 to 839,549
_730
Pf6N2E2_731 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 839,552 to 840,307
_731
Pf6N2E2_732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 840,300 to 840,980
_732
Position (kb)
838
839
840 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 837.315 kb on - strand, within Pf6N2E2_729 at 837.315 kb on - strand, within Pf6N2E2_729 at 837.383 kb on + strand, within Pf6N2E2_729 at 837.384 kb on - strand, within Pf6N2E2_729 at 837.551 kb on + strand, within Pf6N2E2_729 at 837.551 kb on + strand, within Pf6N2E2_729 at 837.551 kb on + strand, within Pf6N2E2_729 at 837.552 kb on - strand, within Pf6N2E2_729 at 837.552 kb on - strand, within Pf6N2E2_729 at 837.552 kb on - strand, within Pf6N2E2_729 at 837.552 kb on - strand, within Pf6N2E2_729 at 837.734 kb on + strand at 837.734 kb on + strand at 837.734 kb on + strand at 837.734 kb on + strand at 837.734 kb on + strand at 837.734 kb on + strand at 837.734 kb on + strand at 837.735 kb on - strand at 837.735 kb on - strand at 837.735 kb on - strand at 837.735 kb on - strand at 837.735 kb on - strand at 837.735 kb on - strand at 837.879 kb on - strand at 837.879 kb on - strand at 837.879 kb on - strand at 837.879 kb on - strand at 838.122 kb on + strand at 838.123 kb on - strand at 838.123 kb on - strand at 838.308 kb on + strand, within Pf6N2E2_730 at 838.309 kb on - strand, within Pf6N2E2_730 at 838.311 kb on + strand, within Pf6N2E2_730 at 838.311 kb on + strand, within Pf6N2E2_730 at 838.512 kb on + strand, within Pf6N2E2_730 at 838.513 kb on - strand, within Pf6N2E2_730 at 838.542 kb on + strand, within Pf6N2E2_730 at 838.542 kb on + strand, within Pf6N2E2_730 at 838.542 kb on + strand, within Pf6N2E2_730 at 838.542 kb on + strand, within Pf6N2E2_730 at 838.542 kb on + strand, within Pf6N2E2_730 at 838.543 kb on - strand, within Pf6N2E2_730 at 838.543 kb on - strand, within Pf6N2E2_730 at 838.543 kb on - strand, within Pf6N2E2_730 at 838.611 kb on + strand, within Pf6N2E2_730 at 838.612 kb on - strand, within Pf6N2E2_730 at 838.612 kb on - strand, within Pf6N2E2_730 at 838.612 kb on - strand, within Pf6N2E2_730 at 838.692 kb on + strand, within Pf6N2E2_730 at 838.692 kb on + strand, within Pf6N2E2_730 at 838.693 kb on - strand, within Pf6N2E2_730 at 838.693 kb on - strand, within Pf6N2E2_730 at 838.693 kb on - strand, within Pf6N2E2_730 at 838.884 kb on + strand, within Pf6N2E2_730 at 838.885 kb on - strand, within Pf6N2E2_730 at 839.071 kb on - strand, within Pf6N2E2_730 at 839.172 kb on + strand, within Pf6N2E2_730 at 839.251 kb on - strand, within Pf6N2E2_730 at 839.337 kb on + strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.338 kb on - strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.361 kb on + strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.362 kb on - strand, within Pf6N2E2_730 at 839.380 kb on + strand, within Pf6N2E2_730 at 839.880 kb on + strand, within Pf6N2E2_731 at 839.880 kb on + strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.881 kb on - strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 839.888 kb on + strand, within Pf6N2E2_731 at 840.298 kb on + strand at 840.298 kb on + strand at 840.305 kb on + strand at 840.305 kb on + strand at 840.306 kb on - strand at 840.306 kb on - strand at 840.396 kb on + strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 837,315 - Pf6N2E2_729 0.82 +0.3 837,315 - Pf6N2E2_729 0.82 -1.6 837,383 + Pf6N2E2_729 0.83 -0.4 837,384 - Pf6N2E2_729 0.83 +0.4 837,551 + Pf6N2E2_729 0.88 +0.5 837,551 + Pf6N2E2_729 0.88 -0.7 837,551 + Pf6N2E2_729 0.88 +1.2 837,552 - Pf6N2E2_729 0.88 +0.9 837,552 - Pf6N2E2_729 0.88 +1.5 837,552 - Pf6N2E2_729 0.88 +0.6 837,552 - Pf6N2E2_729 0.88 -0.2 837,734 + +1.6 837,734 + +1.1 837,734 + +0.3 837,734 + +0.1 837,734 + -1.0 837,734 + -0.1 837,734 + +0.7 837,735 - -1.0 837,735 - +0.9 837,735 - +0.1 837,735 - -0.5 837,735 - -0.7 837,735 - -0.7 837,879 - +0.8 837,879 - -0.4 837,879 - +0.4 837,879 - +0.8 838,122 + -0.1 838,123 - -0.6 838,123 - -0.7 838,308 + Pf6N2E2_730 0.19 -0.1 838,309 - Pf6N2E2_730 0.19 -0.3 838,311 + Pf6N2E2_730 0.19 -1.9 838,311 + Pf6N2E2_730 0.19 +0.0 838,512 + Pf6N2E2_730 0.33 +1.8 838,513 - Pf6N2E2_730 0.33 -1.1 838,542 + Pf6N2E2_730 0.35 -1.9 838,542 + Pf6N2E2_730 0.35 -0.5 838,542 + Pf6N2E2_730 0.35 +1.2 838,542 + Pf6N2E2_730 0.35 -0.5 838,542 + Pf6N2E2_730 0.35 -1.4 838,543 - Pf6N2E2_730 0.35 -0.9 838,543 - Pf6N2E2_730 0.35 -0.4 838,543 - Pf6N2E2_730 0.35 -0.7 838,611 + Pf6N2E2_730 0.39 -0.2 838,612 - Pf6N2E2_730 0.39 +0.7 838,612 - Pf6N2E2_730 0.39 +0.4 838,612 - Pf6N2E2_730 0.39 +0.5 838,692 + Pf6N2E2_730 0.44 +1.4 838,692 + Pf6N2E2_730 0.44 +0.1 838,693 - Pf6N2E2_730 0.44 -0.1 838,693 - Pf6N2E2_730 0.44 +0.2 838,693 - Pf6N2E2_730 0.44 -0.7 838,884 + Pf6N2E2_730 0.57 -0.9 838,885 - Pf6N2E2_730 0.57 +0.2 839,071 - Pf6N2E2_730 0.69 -2.8 839,172 + Pf6N2E2_730 0.75 +0.3 839,251 - Pf6N2E2_730 0.81 -1.0 839,337 + Pf6N2E2_730 0.86 +0.4 839,338 - Pf6N2E2_730 0.86 +1.2 839,338 - Pf6N2E2_730 0.86 -1.7 839,338 - Pf6N2E2_730 0.86 -1.1 839,361 + Pf6N2E2_730 0.88 -1.0 839,361 + Pf6N2E2_730 0.88 -3.1 839,361 + Pf6N2E2_730 0.88 -0.4 839,362 - Pf6N2E2_730 0.88 -2.8 839,362 - Pf6N2E2_730 0.88 +0.9 839,362 - Pf6N2E2_730 0.88 +0.7 839,380 + Pf6N2E2_730 0.89 -0.4 839,880 + Pf6N2E2_731 0.43 +0.5 839,880 + Pf6N2E2_731 0.43 -0.3 839,881 - Pf6N2E2_731 0.44 -0.4 839,881 - Pf6N2E2_731 0.44 -1.2 839,881 - Pf6N2E2_731 0.44 +0.6 839,888 + Pf6N2E2_731 0.44 -1.2 839,888 + Pf6N2E2_731 0.44 +1.2 839,888 + Pf6N2E2_731 0.44 -1.6 839,888 + Pf6N2E2_731 0.44 +0.1 839,888 + Pf6N2E2_731 0.44 +0.7 839,888 + Pf6N2E2_731 0.44 -0.8 839,888 + Pf6N2E2_731 0.44 -1.2 840,298 + -0.2 840,298 + +0.2 840,305 + +0.4 840,305 + -1.0 840,306 - -0.2 840,306 - +1.3 840,396 + Pf6N2E2_732 0.14 +0.9 840,397 - Pf6N2E2_732 0.14 -0.2 840,397 - Pf6N2E2_732 0.14 +0.2 840,528 + Pf6N2E2_732 0.33 -1.9 840,528 + Pf6N2E2_732 0.33 +1.3 840,528 + Pf6N2E2_732 0.33 +0.1 840,528 + Pf6N2E2_732 0.33 +0.6 840,528 + Pf6N2E2_732 0.33 -0.1 840,528 + Pf6N2E2_732 0.33 -0.8 840,528 + Pf6N2E2_732 0.33 -2.3 840,528 + Pf6N2E2_732 0.33 -0.3 840,529 - Pf6N2E2_732 0.34 +0.2 840,529 - Pf6N2E2_732 0.34 -0.8 840,529 - Pf6N2E2_732 0.34 -2.2 840,529 - Pf6N2E2_732 0.34 -0.6 840,529 - Pf6N2E2_732 0.34 +1.1 840,529 - Pf6N2E2_732 0.34 +2.3
Or see this region's nucleotide sequence