Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5049 and Pf6N2E2_5050 overlap by 4 nucleotides Pf6N2E2_5050 and Pf6N2E2_5051 overlap by 9 nucleotides Pf6N2E2_5051 and Pf6N2E2_5052 overlap by 4 nucleotides Pf6N2E2_5052 and Pf6N2E2_5053 are separated by 70 nucleotides
Pf6N2E2_5049 - acetyltransferase, GNAT family, at 5,825,480 to 5,826,049
_5049
Pf6N2E2_5050 - hypothetical protein, at 5,826,046 to 5,826,162
_5050
Pf6N2E2_5051 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 5,826,154 to 5,827,023
_5051
Pf6N2E2_5052 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 5,827,020 to 5,827,613
_5052
Pf6N2E2_5053 - putative exported protein, at 5,827,684 to 5,828,616
_5053
Position (kb) 5827
5828 Strain fitness (log2 ratio) -2
-1
0
1 at 5826.030 kb on + strand at 5826.064 kb on + strand, within Pf6N2E2_5050 at 5826.064 kb on + strand, within Pf6N2E2_5050 at 5826.065 kb on - strand, within Pf6N2E2_5050 at 5826.065 kb on - strand, within Pf6N2E2_5050 at 5826.115 kb on + strand, within Pf6N2E2_5050 at 5826.116 kb on - strand, within Pf6N2E2_5050 at 5826.379 kb on + strand, within Pf6N2E2_5051 at 5826.380 kb on - strand, within Pf6N2E2_5051 at 5826.380 kb on - strand, within Pf6N2E2_5051 at 5826.481 kb on + strand, within Pf6N2E2_5051 at 5826.658 kb on + strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.659 kb on - strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.688 kb on + strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.689 kb on - strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.742 kb on + strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.743 kb on - strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.822 kb on + strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.823 kb on - strand, within Pf6N2E2_5051 at 5826.893 kb on - strand, within Pf6N2E2_5051 at 5827.542 kb on - strand, within Pf6N2E2_5052 at 5827.658 kb on + strand at 5827.659 kb on - strand at 5827.805 kb on + strand, within Pf6N2E2_5053 at 5827.806 kb on - strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.916 kb on + strand, within Pf6N2E2_5053 at 5827.917 kb on - strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.937 kb on + strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5827.938 kb on - strand, within Pf6N2E2_5053 at 5828.000 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.066 kb on + strand, within Pf6N2E2_5053 at 5828.067 kb on - strand, within Pf6N2E2_5053 at 5828.072 kb on + strand, within Pf6N2E2_5053 at 5828.242 kb on + strand, within Pf6N2E2_5053 at 5828.435 kb on + strand, within Pf6N2E2_5053 at 5828.435 kb on + strand, within Pf6N2E2_5053 at 5828.436 kb on - strand, within Pf6N2E2_5053 at 5828.436 kb on - strand, within Pf6N2E2_5053 at 5828.507 kb on - strand, within Pf6N2E2_5053
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,826,030 + +0.2 5,826,064 + Pf6N2E2_5050 0.15 -0.8 5,826,064 + Pf6N2E2_5050 0.15 +0.6 5,826,065 - Pf6N2E2_5050 0.16 +0.6 5,826,065 - Pf6N2E2_5050 0.16 -0.2 5,826,115 + Pf6N2E2_5050 0.59 -0.0 5,826,116 - Pf6N2E2_5050 0.60 +0.4 5,826,379 + Pf6N2E2_5051 0.26 -0.1 5,826,380 - Pf6N2E2_5051 0.26 +0.2 5,826,380 - Pf6N2E2_5051 0.26 -0.4 5,826,481 + Pf6N2E2_5051 0.38 +0.3 5,826,658 + Pf6N2E2_5051 0.58 -0.8 5,826,659 - Pf6N2E2_5051 0.58 +0.3 5,826,659 - Pf6N2E2_5051 0.58 -0.6 5,826,659 - Pf6N2E2_5051 0.58 +0.1 5,826,659 - Pf6N2E2_5051 0.58 -1.2 5,826,688 + Pf6N2E2_5051 0.61 +0.0 5,826,688 + Pf6N2E2_5051 0.61 +0.3 5,826,688 + Pf6N2E2_5051 0.61 +0.3 5,826,688 + Pf6N2E2_5051 0.61 +1.7 5,826,688 + Pf6N2E2_5051 0.61 -0.3 5,826,688 + Pf6N2E2_5051 0.61 +1.2 5,826,689 - Pf6N2E2_5051 0.61 +0.9 5,826,689 - Pf6N2E2_5051 0.61 +0.0 5,826,689 - Pf6N2E2_5051 0.61 +1.6 5,826,689 - Pf6N2E2_5051 0.61 -0.3 5,826,742 + Pf6N2E2_5051 0.68 -1.1 5,826,742 + Pf6N2E2_5051 0.68 +0.2 5,826,742 + Pf6N2E2_5051 0.68 -1.1 5,826,743 - Pf6N2E2_5051 0.68 +0.9 5,826,743 - Pf6N2E2_5051 0.68 +1.3 5,826,743 - Pf6N2E2_5051 0.68 -0.4 5,826,743 - Pf6N2E2_5051 0.68 +0.0 5,826,822 + Pf6N2E2_5051 0.77 -1.0 5,826,822 + Pf6N2E2_5051 0.77 +0.0 5,826,822 + Pf6N2E2_5051 0.77 -1.6 5,826,822 + Pf6N2E2_5051 0.77 -0.7 5,826,822 + Pf6N2E2_5051 0.77 +0.7 5,826,823 - Pf6N2E2_5051 0.77 -0.6 5,826,823 - Pf6N2E2_5051 0.77 +0.6 5,826,823 - Pf6N2E2_5051 0.77 -0.9 5,826,823 - Pf6N2E2_5051 0.77 +1.0 5,826,823 - Pf6N2E2_5051 0.77 -0.1 5,826,823 - Pf6N2E2_5051 0.77 -0.9 5,826,893 - Pf6N2E2_5051 0.85 +0.6 5,827,542 - Pf6N2E2_5052 0.88 -0.1 5,827,658 + -1.6 5,827,659 - +0.4 5,827,805 + Pf6N2E2_5053 0.13 +0.6 5,827,806 - Pf6N2E2_5053 0.13 -1.0 5,827,916 + Pf6N2E2_5053 0.25 +0.7 5,827,916 + Pf6N2E2_5053 0.25 +0.9 5,827,916 + Pf6N2E2_5053 0.25 +0.0 5,827,917 - Pf6N2E2_5053 0.25 +1.4 5,827,937 + Pf6N2E2_5053 0.27 +0.4 5,827,937 + Pf6N2E2_5053 0.27 +0.3 5,827,937 + Pf6N2E2_5053 0.27 +1.2 5,827,937 + Pf6N2E2_5053 0.27 -0.2 5,827,937 + Pf6N2E2_5053 0.27 -0.1 5,827,938 - Pf6N2E2_5053 0.27 +0.9 5,827,938 - Pf6N2E2_5053 0.27 +0.7 5,827,938 - Pf6N2E2_5053 0.27 +0.6 5,827,938 - Pf6N2E2_5053 0.27 +1.2 5,827,938 - Pf6N2E2_5053 0.27 +1.5 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 +0.0 5,827,938 - Pf6N2E2_5053 0.27 -0.6 5,827,938 - Pf6N2E2_5053 0.27 +0.2 5,827,938 - Pf6N2E2_5053 0.27 -0.9 5,827,938 - Pf6N2E2_5053 0.27 -1.9 5,828,000 + Pf6N2E2_5053 0.34 +1.2 5,828,066 + Pf6N2E2_5053 0.41 -0.6 5,828,066 + Pf6N2E2_5053 0.41 +0.4 5,828,066 + Pf6N2E2_5053 0.41 +0.5 5,828,066 + Pf6N2E2_5053 0.41 +1.1 5,828,067 - Pf6N2E2_5053 0.41 +0.1 5,828,072 + Pf6N2E2_5053 0.42 +0.8 5,828,242 + Pf6N2E2_5053 0.60 -2.3 5,828,435 + Pf6N2E2_5053 0.80 -0.7 5,828,435 + Pf6N2E2_5053 0.80 -0.9 5,828,436 - Pf6N2E2_5053 0.81 -1.4 5,828,436 - Pf6N2E2_5053 0.81 +1.2 5,828,507 - Pf6N2E2_5053 0.88 -1.0
Or see this region's nucleotide sequence