Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4581 and Pf6N2E2_4582 are separated by 155 nucleotides Pf6N2E2_4582 and Pf6N2E2_4583 are separated by 57 nucleotides Pf6N2E2_4583 and Pf6N2E2_4584 overlap by 4 nucleotides Pf6N2E2_4584 and Pf6N2E2_4585 overlap by 4 nucleotides
Pf6N2E2_4581 - FIG015547: peptidase, M16 family, at 5,311,596 to 5,312,951
_4581
Pf6N2E2_4582 - hypothetical protein, at 5,313,107 to 5,313,238
_4582
Pf6N2E2_4583 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 5,313,296 to 5,314,729
_4583
Pf6N2E2_4584 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 5,314,726 to 5,315,397
_4584
Pf6N2E2_4585 - Cell division protein FtsX, at 5,315,394 to 5,316,416
_4585
Position (kb) 5313
5314
5315 Strain fitness (log2 ratio) -2
-1
0
1 at 5312.330 kb on - strand, within Pf6N2E2_4581 at 5312.450 kb on - strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.761 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5313.011 kb on + strand at 5313.057 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.244 kb on + strand at 5313.244 kb on + strand at 5314.851 kb on + strand, within Pf6N2E2_4584 at 5314.851 kb on + strand, within Pf6N2E2_4584 at 5314.951 kb on + strand, within Pf6N2E2_4584 at 5314.952 kb on - strand, within Pf6N2E2_4584 at 5315.261 kb on + strand, within Pf6N2E2_4584 at 5315.331 kb on + strand at 5315.393 kb on + strand at 5315.393 kb on + strand at 5315.393 kb on + strand at 5315.402 kb on + strand at 5315.403 kb on - strand at 5315.591 kb on + strand, within Pf6N2E2_4585 at 5315.591 kb on + strand, within Pf6N2E2_4585 at 5315.703 kb on + strand, within Pf6N2E2_4585
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,312,330 - Pf6N2E2_4581 0.54 -0.3 5,312,450 - Pf6N2E2_4581 0.63 -1.6 5,312,608 + Pf6N2E2_4581 0.75 +0.1 5,312,608 + Pf6N2E2_4581 0.75 -2.6 5,312,609 - Pf6N2E2_4581 0.75 -1.8 5,312,609 - Pf6N2E2_4581 0.75 +0.2 5,312,609 - Pf6N2E2_4581 0.75 +0.3 5,312,609 - Pf6N2E2_4581 0.75 -1.0 5,312,611 + Pf6N2E2_4581 0.75 -1.6 5,312,611 + Pf6N2E2_4581 0.75 -1.4 5,312,612 - Pf6N2E2_4581 0.75 -0.2 5,312,612 - Pf6N2E2_4581 0.75 +0.4 5,312,761 + Pf6N2E2_4581 0.86 -2.4 5,312,779 + Pf6N2E2_4581 0.87 -2.1 5,312,779 + Pf6N2E2_4581 0.87 -0.4 5,312,779 + Pf6N2E2_4581 0.87 -0.7 5,313,011 + -2.2 5,313,057 + -1.1 5,313,240 + -2.2 5,313,240 + -1.0 5,313,240 + +0.7 5,313,240 + +0.8 5,313,244 + +0.8 5,313,244 + +0.3 5,314,851 + Pf6N2E2_4584 0.19 -0.9 5,314,851 + Pf6N2E2_4584 0.19 -2.6 5,314,951 + Pf6N2E2_4584 0.33 -0.9 5,314,952 - Pf6N2E2_4584 0.34 -1.3 5,315,261 + Pf6N2E2_4584 0.80 -2.6 5,315,331 + -0.4 5,315,393 + -0.5 5,315,393 + -0.5 5,315,393 + -1.0 5,315,402 + -2.4 5,315,403 - -1.2 5,315,591 + Pf6N2E2_4585 0.19 -2.2 5,315,591 + Pf6N2E2_4585 0.19 -2.1 5,315,703 + Pf6N2E2_4585 0.30 -0.9
Or see this region's nucleotide sequence