Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3705

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3703 and Pf6N2E2_3704 are separated by 70 nucleotidesPf6N2E2_3704 and Pf6N2E2_3705 are separated by 11 nucleotidesPf6N2E2_3705 and Pf6N2E2_3706 are separated by 27 nucleotidesPf6N2E2_3706 and Pf6N2E2_3707 are separated by 37 nucleotides Pf6N2E2_3703 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,317,500 to 4,318,249 _3703 Pf6N2E2_3704 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 4,318,320 to 4,319,081 _3704 Pf6N2E2_3705 - Putative sugar transporter, at 4,319,093 to 4,320,433 _3705 Pf6N2E2_3706 - Putative polysaccharide deacetylase, at 4,320,461 to 4,321,342 _3706 Pf6N2E2_3707 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases, at 4,321,380 to 4,322,753 _3707 Position (kb) 4319 4320 4321Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4318.174 kb on - strand, within Pf6N2E2_3703at 4318.284 kb on + strandat 4318.378 kb on + strandat 4318.378 kb on + strandat 4318.379 kb on - strandat 4318.593 kb on + strand, within Pf6N2E2_3704at 4318.593 kb on + strand, within Pf6N2E2_3704at 4318.593 kb on + strand, within Pf6N2E2_3704at 4318.624 kb on + strand, within Pf6N2E2_3704at 4318.648 kb on + strand, within Pf6N2E2_3704at 4318.648 kb on + strand, within Pf6N2E2_3704at 4318.649 kb on - strand, within Pf6N2E2_3704at 4318.649 kb on - strand, within Pf6N2E2_3704at 4318.649 kb on - strand, within Pf6N2E2_3704at 4318.649 kb on - strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.650 kb on + strand, within Pf6N2E2_3704at 4318.651 kb on - strand, within Pf6N2E2_3704at 4318.651 kb on - strand, within Pf6N2E2_3704at 4318.651 kb on - strand, within Pf6N2E2_3704at 4318.702 kb on + strand, within Pf6N2E2_3704at 4318.702 kb on + strand, within Pf6N2E2_3704at 4318.702 kb on + strand, within Pf6N2E2_3704at 4318.703 kb on - strand, within Pf6N2E2_3704at 4318.703 kb on - strand, within Pf6N2E2_3704at 4318.703 kb on - strand, within Pf6N2E2_3704at 4318.703 kb on - strand, within Pf6N2E2_3704at 4318.981 kb on + strand, within Pf6N2E2_3704at 4318.982 kb on - strand, within Pf6N2E2_3704at 4318.982 kb on - strand, within Pf6N2E2_3704at 4319.014 kb on + strandat 4319.014 kb on + strandat 4319.094 kb on + strandat 4319.094 kb on + strandat 4319.094 kb on + strandat 4319.094 kb on + strandat 4319.094 kb on + strandat 4319.094 kb on + strandat 4319.095 kb on - strandat 4319.095 kb on - strandat 4319.095 kb on - strandat 4319.095 kb on - strandat 4319.095 kb on - strandat 4319.172 kb on + strandat 4319.334 kb on + strand, within Pf6N2E2_3705at 4319.367 kb on + strand, within Pf6N2E2_3705at 4319.509 kb on - strand, within Pf6N2E2_3705at 4319.560 kb on + strand, within Pf6N2E2_3705at 4319.560 kb on + strand, within Pf6N2E2_3705at 4319.560 kb on + strand, within Pf6N2E2_3705at 4319.560 kb on + strand, within Pf6N2E2_3705at 4319.561 kb on - strand, within Pf6N2E2_3705at 4319.561 kb on - strand, within Pf6N2E2_3705at 4319.561 kb on - strand, within Pf6N2E2_3705at 4319.561 kb on - strand, within Pf6N2E2_3705at 4319.561 kb on - strand, within Pf6N2E2_3705at 4319.712 kb on + strand, within Pf6N2E2_3705at 4319.713 kb on - strand, within Pf6N2E2_3705at 4319.713 kb on - strand, within Pf6N2E2_3705at 4319.724 kb on + strand, within Pf6N2E2_3705at 4319.724 kb on + strand, within Pf6N2E2_3705at 4319.724 kb on + strand, within Pf6N2E2_3705at 4319.725 kb on - strand, within Pf6N2E2_3705at 4319.725 kb on - strand, within Pf6N2E2_3705at 4319.866 kb on + strand, within Pf6N2E2_3705at 4319.961 kb on + strand, within Pf6N2E2_3705at 4319.961 kb on + strand, within Pf6N2E2_3705at 4319.961 kb on + strand, within Pf6N2E2_3705at 4319.961 kb on + strand, within Pf6N2E2_3705at 4319.962 kb on - strand, within Pf6N2E2_3705at 4319.962 kb on - strand, within Pf6N2E2_3705at 4319.962 kb on - strand, within Pf6N2E2_3705at 4319.962 kb on - strand, within Pf6N2E2_3705at 4319.962 kb on - strand, within Pf6N2E2_3705at 4320.024 kb on + strand, within Pf6N2E2_3705at 4320.024 kb on + strand, within Pf6N2E2_3705at 4320.024 kb on + strand, within Pf6N2E2_3705at 4320.025 kb on - strand, within Pf6N2E2_3705at 4320.025 kb on - strand, within Pf6N2E2_3705at 4320.025 kb on - strand, within Pf6N2E2_3705at 4320.321 kb on + strandat 4320.423 kb on + strandat 4320.424 kb on - strandat 4320.450 kb on + strandat 4320.450 kb on + strandat 4320.451 kb on - strandat 4320.451 kb on - strandat 4320.451 kb on - strandat 4320.642 kb on + strand, within Pf6N2E2_3706at 4320.753 kb on + strand, within Pf6N2E2_3706at 4320.834 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.870 kb on + strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.871 kb on - strand, within Pf6N2E2_3706at 4320.873 kb on + strand, within Pf6N2E2_3706at 4320.873 kb on + strand, within Pf6N2E2_3706at 4320.874 kb on - strand, within Pf6N2E2_3706at 4320.874 kb on - strand, within Pf6N2E2_3706at 4320.874 kb on - strand, within Pf6N2E2_3706at 4320.909 kb on + strand, within Pf6N2E2_3706at 4320.909 kb on + strand, within Pf6N2E2_3706at 4320.910 kb on - strand, within Pf6N2E2_3706at 4321.024 kb on + strand, within Pf6N2E2_3706at 4321.078 kb on + strand, within Pf6N2E2_3706at 4321.078 kb on + strand, within Pf6N2E2_3706at 4321.078 kb on + strand, within Pf6N2E2_3706at 4321.079 kb on - strand, within Pf6N2E2_3706at 4321.079 kb on - strand, within Pf6N2E2_3706at 4321.250 kb on - strand, within Pf6N2E2_3706at 4321.250 kb on - strand, within Pf6N2E2_3706at 4321.305 kb on - strandat 4321.331 kb on - strandat 4321.331 kb on - strandat 4321.370 kb on + strandat 4321.371 kb on - strandat 4321.371 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,318,174 - Pf6N2E2_3703 0.90 -0.4
4,318,284 + +1.2
4,318,378 + -1.0
4,318,378 + -1.0
4,318,379 - -0.0
4,318,593 + Pf6N2E2_3704 0.36 -0.1
4,318,593 + Pf6N2E2_3704 0.36 -3.4
4,318,593 + Pf6N2E2_3704 0.36 +0.9
4,318,624 + Pf6N2E2_3704 0.40 +0.9
4,318,648 + Pf6N2E2_3704 0.43 -3.2
4,318,648 + Pf6N2E2_3704 0.43 +1.3
4,318,649 - Pf6N2E2_3704 0.43 +2.3
4,318,649 - Pf6N2E2_3704 0.43 -1.6
4,318,649 - Pf6N2E2_3704 0.43 -0.4
4,318,649 - Pf6N2E2_3704 0.43 +0.8
4,318,650 + Pf6N2E2_3704 0.43 -0.1
4,318,650 + Pf6N2E2_3704 0.43 -0.6
4,318,650 + Pf6N2E2_3704 0.43 +0.8
4,318,650 + Pf6N2E2_3704 0.43 -0.1
4,318,650 + Pf6N2E2_3704 0.43 -0.3
4,318,650 + Pf6N2E2_3704 0.43 -0.9
4,318,650 + Pf6N2E2_3704 0.43 -0.5
4,318,651 - Pf6N2E2_3704 0.43 -1.8
4,318,651 - Pf6N2E2_3704 0.43 -1.7
4,318,651 - Pf6N2E2_3704 0.43 +0.1
4,318,702 + Pf6N2E2_3704 0.50 +0.2
4,318,702 + Pf6N2E2_3704 0.50 +0.8
4,318,702 + Pf6N2E2_3704 0.50 +0.0
4,318,703 - Pf6N2E2_3704 0.50 +0.7
4,318,703 - Pf6N2E2_3704 0.50 +0.8
4,318,703 - Pf6N2E2_3704 0.50 -0.6
4,318,703 - Pf6N2E2_3704 0.50 -1.4
4,318,981 + Pf6N2E2_3704 0.87 -0.4
4,318,982 - Pf6N2E2_3704 0.87 -1.1
4,318,982 - Pf6N2E2_3704 0.87 -1.4
4,319,014 + -1.0
4,319,014 + -0.6
4,319,094 + +1.6
4,319,094 + -2.4
4,319,094 + +1.3
4,319,094 + +0.9
4,319,094 + -1.6
4,319,094 + -0.8
4,319,095 - +1.3
4,319,095 - +1.5
4,319,095 - +1.0
4,319,095 - -2.1
4,319,095 - +0.4
4,319,172 + -0.6
4,319,334 + Pf6N2E2_3705 0.18 +0.9
4,319,367 + Pf6N2E2_3705 0.20 -2.5
4,319,509 - Pf6N2E2_3705 0.31 +0.1
4,319,560 + Pf6N2E2_3705 0.35 -0.7
4,319,560 + Pf6N2E2_3705 0.35 -2.1
4,319,560 + Pf6N2E2_3705 0.35 -0.2
4,319,560 + Pf6N2E2_3705 0.35 -0.3
4,319,561 - Pf6N2E2_3705 0.35 -1.2
4,319,561 - Pf6N2E2_3705 0.35 +0.9
4,319,561 - Pf6N2E2_3705 0.35 +0.6
4,319,561 - Pf6N2E2_3705 0.35 -1.0
4,319,561 - Pf6N2E2_3705 0.35 -0.5
4,319,712 + Pf6N2E2_3705 0.46 -0.6
4,319,713 - Pf6N2E2_3705 0.46 -0.9
4,319,713 - Pf6N2E2_3705 0.46 +0.3
4,319,724 + Pf6N2E2_3705 0.47 -1.2
4,319,724 + Pf6N2E2_3705 0.47 +0.5
4,319,724 + Pf6N2E2_3705 0.47 -0.4
4,319,725 - Pf6N2E2_3705 0.47 -0.1
4,319,725 - Pf6N2E2_3705 0.47 -0.2
4,319,866 + Pf6N2E2_3705 0.58 +0.2
4,319,961 + Pf6N2E2_3705 0.65 -1.0
4,319,961 + Pf6N2E2_3705 0.65 -1.3
4,319,961 + Pf6N2E2_3705 0.65 -0.1
4,319,961 + Pf6N2E2_3705 0.65 +0.6
4,319,962 - Pf6N2E2_3705 0.65 -0.6
4,319,962 - Pf6N2E2_3705 0.65 -0.4
4,319,962 - Pf6N2E2_3705 0.65 +1.8
4,319,962 - Pf6N2E2_3705 0.65 -2.1
4,319,962 - Pf6N2E2_3705 0.65 -0.2
4,320,024 + Pf6N2E2_3705 0.69 -0.7
4,320,024 + Pf6N2E2_3705 0.69 -1.4
4,320,024 + Pf6N2E2_3705 0.69 -2.0
4,320,025 - Pf6N2E2_3705 0.70 -0.2
4,320,025 - Pf6N2E2_3705 0.70 +1.1
4,320,025 - Pf6N2E2_3705 0.70 +1.3
4,320,321 + +0.8
4,320,423 + -0.2
4,320,424 - +2.2
4,320,450 + -1.4
4,320,450 + -0.5
4,320,451 - -2.5
4,320,451 - -0.3
4,320,451 - +0.2
4,320,642 + Pf6N2E2_3706 0.21 -0.9
4,320,753 + Pf6N2E2_3706 0.33 +1.7
4,320,834 + Pf6N2E2_3706 0.42 -1.2
4,320,870 + Pf6N2E2_3706 0.46 -1.0
4,320,870 + Pf6N2E2_3706 0.46 +0.5
4,320,870 + Pf6N2E2_3706 0.46 +0.6
4,320,870 + Pf6N2E2_3706 0.46 +1.3
4,320,870 + Pf6N2E2_3706 0.46 +0.7
4,320,870 + Pf6N2E2_3706 0.46 +2.6
4,320,870 + Pf6N2E2_3706 0.46 -0.7
4,320,871 - Pf6N2E2_3706 0.46 +0.7
4,320,871 - Pf6N2E2_3706 0.46 -0.4
4,320,871 - Pf6N2E2_3706 0.46 +0.1
4,320,871 - Pf6N2E2_3706 0.46 +0.5
4,320,871 - Pf6N2E2_3706 0.46 -0.9
4,320,871 - Pf6N2E2_3706 0.46 -0.6
4,320,871 - Pf6N2E2_3706 0.46 -1.8
4,320,871 - Pf6N2E2_3706 0.46 +0.1
4,320,871 - Pf6N2E2_3706 0.46 -1.4
4,320,873 + Pf6N2E2_3706 0.47 -2.5
4,320,873 + Pf6N2E2_3706 0.47 -0.9
4,320,874 - Pf6N2E2_3706 0.47 +0.1
4,320,874 - Pf6N2E2_3706 0.47 -0.7
4,320,874 - Pf6N2E2_3706 0.47 -1.4
4,320,909 + Pf6N2E2_3706 0.51 -1.3
4,320,909 + Pf6N2E2_3706 0.51 -1.1
4,320,910 - Pf6N2E2_3706 0.51 +0.6
4,321,024 + Pf6N2E2_3706 0.64 +1.2
4,321,078 + Pf6N2E2_3706 0.70 -0.2
4,321,078 + Pf6N2E2_3706 0.70 +0.2
4,321,078 + Pf6N2E2_3706 0.70 -0.3
4,321,079 - Pf6N2E2_3706 0.70 -2.1
4,321,079 - Pf6N2E2_3706 0.70 +2.8
4,321,250 - Pf6N2E2_3706 0.89 -2.6
4,321,250 - Pf6N2E2_3706 0.89 -0.1
4,321,305 - -0.6
4,321,331 - -0.1
4,321,331 - -1.6
4,321,370 + -0.4
4,321,371 - -2.1
4,321,371 - -2.1

Or see this region's nucleotide sequence