Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3110 and Pf6N2E2_3111 overlap by 4 nucleotides Pf6N2E2_3111 and Pf6N2E2_3112 are separated by 84 nucleotides Pf6N2E2_3112 and Pf6N2E2_3113 are separated by 36 nucleotides Pf6N2E2_3113 and Pf6N2E2_3114 are separated by 144 nucleotides
Pf6N2E2_3110 - Putative lipoprotein in cluster with COG2110, at 3,598,822 to 3,599,151
_3110
Pf6N2E2_3111 - Hydrolase of the alpha/beta superfamily in cluster with COG2110, at 3,599,148 to 3,600,053
_3111
Pf6N2E2_3112 - FIG00953322: hypothetical protein, at 3,600,138 to 3,601,415
_3112
Pf6N2E2_3113 - Putative lipoprotein, specific for Pseudomonas, in cluster with COG2110, at 3,601,452 to 3,602,006
_3113
Pf6N2E2_3114 - Transcriptional regulator, TetR family, at 3,602,151 to 3,602,867
_3114
Position (kb)
3600
3601
3602 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3599.341 kb on + strand, within Pf6N2E2_3111 at 3599.342 kb on - strand, within Pf6N2E2_3111 at 3599.342 kb on - strand, within Pf6N2E2_3111 at 3599.342 kb on - strand, within Pf6N2E2_3111 at 3599.392 kb on + strand, within Pf6N2E2_3111 at 3599.392 kb on + strand, within Pf6N2E2_3111 at 3599.392 kb on + strand, within Pf6N2E2_3111 at 3599.393 kb on - strand, within Pf6N2E2_3111 at 3599.393 kb on - strand, within Pf6N2E2_3111 at 3599.393 kb on - strand, within Pf6N2E2_3111 at 3599.393 kb on - strand, within Pf6N2E2_3111 at 3599.501 kb on + strand, within Pf6N2E2_3111 at 3599.501 kb on + strand, within Pf6N2E2_3111 at 3599.501 kb on + strand, within Pf6N2E2_3111 at 3599.501 kb on + strand, within Pf6N2E2_3111 at 3599.501 kb on + strand, within Pf6N2E2_3111 at 3599.502 kb on - strand, within Pf6N2E2_3111 at 3599.707 kb on + strand, within Pf6N2E2_3111 at 3599.708 kb on - strand, within Pf6N2E2_3111 at 3599.990 kb on - strand at 3599.990 kb on - strand at 3600.027 kb on + strand at 3600.027 kb on + strand at 3600.028 kb on - strand at 3600.147 kb on - strand at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.299 kb on + strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.300 kb on - strand, within Pf6N2E2_3112 at 3600.383 kb on - strand, within Pf6N2E2_3112 at 3600.383 kb on - strand, within Pf6N2E2_3112 at 3600.383 kb on - strand, within Pf6N2E2_3112 at 3600.388 kb on + strand, within Pf6N2E2_3112 at 3600.388 kb on + strand, within Pf6N2E2_3112 at 3600.389 kb on - strand, within Pf6N2E2_3112 at 3600.389 kb on - strand at 3600.389 kb on - strand, within Pf6N2E2_3112 at 3600.389 kb on - strand, within Pf6N2E2_3112 at 3600.428 kb on + strand, within Pf6N2E2_3112 at 3600.482 kb on - strand, within Pf6N2E2_3112 at 3600.482 kb on - strand, within Pf6N2E2_3112 at 3600.482 kb on - strand, within Pf6N2E2_3112 at 3600.805 kb on + strand, within Pf6N2E2_3112 at 3600.806 kb on - strand, within Pf6N2E2_3112 at 3600.806 kb on - strand, within Pf6N2E2_3112 at 3600.806 kb on - strand, within Pf6N2E2_3112 at 3601.255 kb on + strand, within Pf6N2E2_3112 at 3601.255 kb on + strand, within Pf6N2E2_3112 at 3601.255 kb on + strand, within Pf6N2E2_3112 at 3601.255 kb on + strand, within Pf6N2E2_3112 at 3601.256 kb on - strand, within Pf6N2E2_3112 at 3601.256 kb on - strand, within Pf6N2E2_3112 at 3601.258 kb on + strand, within Pf6N2E2_3112 at 3601.259 kb on - strand, within Pf6N2E2_3112 at 3601.259 kb on - strand, within Pf6N2E2_3112 at 3601.259 kb on - strand, within Pf6N2E2_3112 at 3601.259 kb on - strand, within Pf6N2E2_3112 at 3601.405 kb on + strand at 3601.453 kb on + strand at 3601.454 kb on - strand at 3601.454 kb on - strand at 3601.525 kb on + strand, within Pf6N2E2_3113 at 3601.649 kb on - strand, within Pf6N2E2_3113 at 3601.649 kb on - strand, within Pf6N2E2_3113 at 3601.696 kb on + strand, within Pf6N2E2_3113 at 3601.735 kb on + strand, within Pf6N2E2_3113 at 3601.735 kb on + strand, within Pf6N2E2_3113 at 3601.735 kb on + strand, within Pf6N2E2_3113 at 3601.735 kb on + strand, within Pf6N2E2_3113 at 3601.735 kb on + strand, within Pf6N2E2_3113 at 3601.736 kb on - strand, within Pf6N2E2_3113 at 3601.736 kb on - strand, within Pf6N2E2_3113 at 3601.736 kb on - strand, within Pf6N2E2_3113 at 3601.804 kb on + strand, within Pf6N2E2_3113 at 3601.804 kb on + strand, within Pf6N2E2_3113 at 3601.804 kb on + strand, within Pf6N2E2_3113 at 3601.805 kb on - strand, within Pf6N2E2_3113 at 3602.018 kb on - strand at 3602.133 kb on + strand at 3602.134 kb on - strand at 3602.134 kb on - strand at 3602.152 kb on + strand at 3602.152 kb on + strand at 3602.152 kb on + strand at 3602.153 kb on - strand at 3602.166 kb on + strand at 3602.166 kb on + strand at 3602.167 kb on - strand at 3602.180 kb on - strand at 3602.180 kb on - strand at 3602.180 kb on - strand at 3602.180 kb on - strand at 3602.180 kb on - strand at 3602.201 kb on + strand at 3602.201 kb on + strand at 3602.201 kb on + strand at 3602.201 kb on + strand at 3602.202 kb on - strand at 3602.202 kb on - strand at 3602.202 kb on - strand at 3602.245 kb on + strand, within Pf6N2E2_3114 at 3602.310 kb on + strand, within Pf6N2E2_3114 at 3602.311 kb on - strand, within Pf6N2E2_3114
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,599,341 + Pf6N2E2_3111 0.21 +1.1 3,599,342 - Pf6N2E2_3111 0.21 -0.9 3,599,342 - Pf6N2E2_3111 0.21 -1.1 3,599,342 - Pf6N2E2_3111 0.21 -0.0 3,599,392 + Pf6N2E2_3111 0.27 +1.7 3,599,392 + Pf6N2E2_3111 0.27 -0.7 3,599,392 + Pf6N2E2_3111 0.27 -1.7 3,599,393 - Pf6N2E2_3111 0.27 -0.0 3,599,393 - Pf6N2E2_3111 0.27 +0.2 3,599,393 - Pf6N2E2_3111 0.27 -0.9 3,599,393 - Pf6N2E2_3111 0.27 -0.8 3,599,501 + Pf6N2E2_3111 0.39 +0.7 3,599,501 + Pf6N2E2_3111 0.39 +0.2 3,599,501 + Pf6N2E2_3111 0.39 +1.5 3,599,501 + Pf6N2E2_3111 0.39 -1.0 3,599,501 + Pf6N2E2_3111 0.39 -1.5 3,599,502 - Pf6N2E2_3111 0.39 +0.6 3,599,707 + Pf6N2E2_3111 0.62 -1.3 3,599,708 - Pf6N2E2_3111 0.62 -0.8 3,599,990 - -0.8 3,599,990 - +0.2 3,600,027 + +1.9 3,600,027 + -0.1 3,600,028 - -1.4 3,600,147 - +0.2 3,600,299 + Pf6N2E2_3112 0.13 -0.2 3,600,299 + Pf6N2E2_3112 0.13 +0.2 3,600,299 + Pf6N2E2_3112 0.13 +1.0 3,600,299 + Pf6N2E2_3112 0.13 -1.1 3,600,299 + Pf6N2E2_3112 0.13 -0.6 3,600,299 + Pf6N2E2_3112 0.13 -0.5 3,600,299 + Pf6N2E2_3112 0.13 +0.5 3,600,299 + Pf6N2E2_3112 0.13 +2.6 3,600,299 + Pf6N2E2_3112 0.13 +0.4 3,600,299 + Pf6N2E2_3112 0.13 +0.4 3,600,299 + Pf6N2E2_3112 0.13 -0.1 3,600,299 + Pf6N2E2_3112 0.13 +0.3 3,600,300 - Pf6N2E2_3112 0.13 +0.3 3,600,300 - Pf6N2E2_3112 0.13 +0.3 3,600,300 - Pf6N2E2_3112 0.13 +0.8 3,600,300 - Pf6N2E2_3112 0.13 -0.5 3,600,300 - Pf6N2E2_3112 0.13 +0.6 3,600,300 - Pf6N2E2_3112 0.13 -2.3 3,600,300 - Pf6N2E2_3112 0.13 -2.4 3,600,300 - Pf6N2E2_3112 0.13 -0.5 3,600,383 - Pf6N2E2_3112 0.19 -0.5 3,600,383 - Pf6N2E2_3112 0.19 +0.6 3,600,383 - Pf6N2E2_3112 0.19 +2.0 3,600,388 + Pf6N2E2_3112 0.20 +0.8 3,600,388 + Pf6N2E2_3112 0.20 -1.9 3,600,389 - Pf6N2E2_3112 0.20 -1.2 3,600,389 - +2.3 3,600,389 - Pf6N2E2_3112 0.20 +0.4 3,600,389 - Pf6N2E2_3112 0.20 +1.4 3,600,428 + Pf6N2E2_3112 0.23 -0.9 3,600,482 - Pf6N2E2_3112 0.27 -0.8 3,600,482 - Pf6N2E2_3112 0.27 -1.9 3,600,482 - Pf6N2E2_3112 0.27 -0.2 3,600,805 + Pf6N2E2_3112 0.52 +1.3 3,600,806 - Pf6N2E2_3112 0.52 +1.1 3,600,806 - Pf6N2E2_3112 0.52 +0.7 3,600,806 - Pf6N2E2_3112 0.52 +1.4 3,601,255 + Pf6N2E2_3112 0.87 +1.5 3,601,255 + Pf6N2E2_3112 0.87 +1.5 3,601,255 + Pf6N2E2_3112 0.87 -1.6 3,601,255 + Pf6N2E2_3112 0.87 +0.2 3,601,256 - Pf6N2E2_3112 0.87 +2.6 3,601,256 - Pf6N2E2_3112 0.87 -0.3 3,601,258 + Pf6N2E2_3112 0.88 +1.1 3,601,259 - Pf6N2E2_3112 0.88 +0.9 3,601,259 - Pf6N2E2_3112 0.88 +0.2 3,601,259 - Pf6N2E2_3112 0.88 +1.7 3,601,259 - Pf6N2E2_3112 0.88 +0.8 3,601,405 + -2.0 3,601,453 + +0.4 3,601,454 - -0.6 3,601,454 - +1.0 3,601,525 + Pf6N2E2_3113 0.13 -1.6 3,601,649 - Pf6N2E2_3113 0.35 +0.7 3,601,649 - Pf6N2E2_3113 0.35 +0.5 3,601,696 + Pf6N2E2_3113 0.44 +0.1 3,601,735 + Pf6N2E2_3113 0.51 +1.0 3,601,735 + Pf6N2E2_3113 0.51 +1.2 3,601,735 + Pf6N2E2_3113 0.51 -0.2 3,601,735 + Pf6N2E2_3113 0.51 +0.0 3,601,735 + Pf6N2E2_3113 0.51 +1.3 3,601,736 - Pf6N2E2_3113 0.51 -0.9 3,601,736 - Pf6N2E2_3113 0.51 +1.4 3,601,736 - Pf6N2E2_3113 0.51 -0.0 3,601,804 + Pf6N2E2_3113 0.63 -1.4 3,601,804 + Pf6N2E2_3113 0.63 +0.7 3,601,804 + Pf6N2E2_3113 0.63 -0.9 3,601,805 - Pf6N2E2_3113 0.64 +0.7 3,602,018 - -0.3 3,602,133 + -1.3 3,602,134 - +0.3 3,602,134 - -0.4 3,602,152 + -1.7 3,602,152 + +1.0 3,602,152 + -1.2 3,602,153 - -0.7 3,602,166 + +1.1 3,602,166 + -0.7 3,602,167 - +0.5 3,602,180 - -0.8 3,602,180 - +0.5 3,602,180 - +1.8 3,602,180 - +0.3 3,602,180 - -1.4 3,602,201 + -0.6 3,602,201 + -1.1 3,602,201 + -0.2 3,602,201 + -0.2 3,602,202 - -0.7 3,602,202 - -1.3 3,602,202 - -1.2 3,602,245 + Pf6N2E2_3114 0.13 -0.5 3,602,310 + Pf6N2E2_3114 0.22 +0.8 3,602,311 - Pf6N2E2_3114 0.22 +0.5
Or see this region's nucleotide sequence