Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_294 and Pf6N2E2_295 are separated by 57 nucleotides Pf6N2E2_295 and Pf6N2E2_296 are separated by 81 nucleotides Pf6N2E2_296 and Pf6N2E2_297 are separated by 308 nucleotides
Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124
_294
Pf6N2E2_295 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 364,182 to 364,862
_295
Pf6N2E2_296 - Thioredoxin reductase (EC 1.8.1.9), at 364,944 to 365,903
_296
Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617
_297
Position (kb) 364
365
366 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 363.972 kb on - strand, within Pf6N2E2_294 at 364.097 kb on - strand at 364.147 kb on + strand at 364.148 kb on - strand at 364.153 kb on + strand at 364.153 kb on + strand at 364.153 kb on + strand at 364.154 kb on - strand at 364.159 kb on + strand at 364.159 kb on + strand at 364.160 kb on - strand at 364.160 kb on - strand at 364.160 kb on - strand at 364.231 kb on + strand at 364.232 kb on - strand at 364.419 kb on + strand, within Pf6N2E2_295 at 364.427 kb on - strand, within Pf6N2E2_295 at 364.544 kb on - strand, within Pf6N2E2_295 at 364.606 kb on + strand, within Pf6N2E2_295 at 364.606 kb on + strand, within Pf6N2E2_295 at 364.768 kb on - strand, within Pf6N2E2_295 at 364.867 kb on - strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.878 kb on + strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.879 kb on - strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.969 kb on + strand at 364.970 kb on - strand at 364.970 kb on - strand at 364.970 kb on - strand at 364.970 kb on - strand at 365.015 kb on + strand at 365.015 kb on + strand at 365.015 kb on + strand at 365.016 kb on - strand at 365.016 kb on - strand at 365.016 kb on - strand at 365.110 kb on - strand, within Pf6N2E2_296 at 365.151 kb on + strand, within Pf6N2E2_296 at 365.152 kb on - strand, within Pf6N2E2_296 at 365.169 kb on - strand, within Pf6N2E2_296 at 365.169 kb on - strand, within Pf6N2E2_296 at 365.177 kb on + strand, within Pf6N2E2_296 at 365.187 kb on + strand, within Pf6N2E2_296 at 365.306 kb on + strand, within Pf6N2E2_296 at 365.307 kb on - strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.594 kb on - strand, within Pf6N2E2_296 at 365.652 kb on - strand, within Pf6N2E2_296 at 365.652 kb on - strand, within Pf6N2E2_296 at 365.712 kb on + strand, within Pf6N2E2_296 at 365.833 kb on + strand at 365.833 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 366.109 kb on + strand at 366.137 kb on + strand at 366.162 kb on + strand at 366.164 kb on + strand at 366.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 363,972 - Pf6N2E2_294 0.78 +1.5 364,097 - -1.0 364,147 + -0.4 364,148 - -1.1 364,153 + +0.0 364,153 + +0.2 364,153 + +0.2 364,154 - -1.9 364,159 + -0.3 364,159 + -1.0 364,160 - +1.4 364,160 - +1.5 364,160 - +0.1 364,231 + -0.4 364,232 - +0.9 364,419 + Pf6N2E2_295 0.35 +1.2 364,427 - Pf6N2E2_295 0.36 -0.9 364,544 - Pf6N2E2_295 0.53 +0.8 364,606 + Pf6N2E2_295 0.62 -0.7 364,606 + Pf6N2E2_295 0.62 +1.7 364,768 - Pf6N2E2_295 0.86 +1.3 364,867 - +0.5 364,878 + +1.8 364,878 + +0.7 364,878 + +1.4 364,878 + -0.9 364,878 + -0.3 364,879 - -0.7 364,879 - -1.2 364,879 - +0.0 364,879 - -1.7 364,879 - +1.1 364,879 - +0.4 364,879 - +0.1 364,969 + +0.2 364,969 + +1.9 364,969 + +0.6 364,969 + -0.7 364,970 - -1.6 364,970 - +0.3 364,970 - -0.0 364,970 - -0.0 365,015 + +1.4 365,015 + +0.8 365,015 + -0.4 365,016 - +1.9 365,016 - -2.2 365,016 - +0.2 365,110 - Pf6N2E2_296 0.17 +0.5 365,151 + Pf6N2E2_296 0.22 -1.2 365,152 - Pf6N2E2_296 0.22 +0.6 365,169 - Pf6N2E2_296 0.23 +0.9 365,169 - Pf6N2E2_296 0.23 +0.7 365,177 + Pf6N2E2_296 0.24 -1.5 365,187 + Pf6N2E2_296 0.25 +0.2 365,306 + Pf6N2E2_296 0.38 +0.2 365,307 - Pf6N2E2_296 0.38 -0.6 365,413 + Pf6N2E2_296 0.49 +2.0 365,413 + Pf6N2E2_296 0.49 +1.6 365,413 + Pf6N2E2_296 0.49 -0.1 365,414 - Pf6N2E2_296 0.49 -2.8 365,414 - Pf6N2E2_296 0.49 +0.8 365,414 - Pf6N2E2_296 0.49 +2.4 365,414 - Pf6N2E2_296 0.49 -1.0 365,414 - Pf6N2E2_296 0.49 +0.7 365,414 - Pf6N2E2_296 0.49 +1.7 365,414 - Pf6N2E2_296 0.49 -0.2 365,414 - Pf6N2E2_296 0.49 +1.2 365,414 - Pf6N2E2_296 0.49 +0.6 365,593 + Pf6N2E2_296 0.68 +3.0 365,593 + Pf6N2E2_296 0.68 +0.9 365,593 + Pf6N2E2_296 0.68 -0.7 365,593 + Pf6N2E2_296 0.68 -1.5 365,593 + Pf6N2E2_296 0.68 -1.2 365,593 + Pf6N2E2_296 0.68 +0.4 365,594 - Pf6N2E2_296 0.68 -0.5 365,652 - Pf6N2E2_296 0.74 -1.2 365,652 - Pf6N2E2_296 0.74 -0.9 365,712 + Pf6N2E2_296 0.80 +0.9 365,833 + +0.9 365,833 + -1.8 365,962 + -2.3 365,962 + -2.1 365,962 + +0.1 365,962 + +4.4 365,962 + +1.4 366,109 + -0.8 366,137 + +0.6 366,162 + -0.1 366,164 + -3.2 366,164 + +0.6
Or see this region's nucleotide sequence