Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2565 and Pf6N2E2_2566 are separated by 33 nucleotides Pf6N2E2_2566 and Pf6N2E2_2567 are separated by 49 nucleotides Pf6N2E2_2567 and Pf6N2E2_2568 are separated by 15 nucleotides Pf6N2E2_2568 and Pf6N2E2_2569 overlap by 1 nucleotides
Pf6N2E2_2565 - CheW domain protein, at 3,022,936 to 3,023,874
_2565
Pf6N2E2_2566 - Chromosome (plasmid) partitioning protein ParA, at 3,023,908 to 3,024,699
_2566
Pf6N2E2_2567 - Flagellar motor rotation protein MotB, at 3,024,749 to 3,025,633
_2567
Pf6N2E2_2568 - Flagellar motor rotation protein MotA, at 3,025,649 to 3,026,389
_2568
Pf6N2E2_2569 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,026,389 to 3,027,522
_2569
Position (kb)
3024
3025
3026 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3023.760 kb on - strand, within Pf6N2E2_2565 at 3023.760 kb on - strand, within Pf6N2E2_2565 at 3023.798 kb on - strand at 3023.811 kb on + strand at 3023.909 kb on + strand at 3024.055 kb on - strand, within Pf6N2E2_2566 at 3024.056 kb on + strand, within Pf6N2E2_2566 at 3024.056 kb on + strand, within Pf6N2E2_2566 at 3024.056 kb on + strand, within Pf6N2E2_2566 at 3024.056 kb on + strand, within Pf6N2E2_2566 at 3024.056 kb on + strand, within Pf6N2E2_2566 at 3024.057 kb on - strand, within Pf6N2E2_2566 at 3024.057 kb on - strand, within Pf6N2E2_2566 at 3024.057 kb on - strand, within Pf6N2E2_2566 at 3024.057 kb on - strand, within Pf6N2E2_2566 at 3024.057 kb on - strand, within Pf6N2E2_2566 at 3024.080 kb on + strand, within Pf6N2E2_2566 at 3024.081 kb on - strand, within Pf6N2E2_2566 at 3024.081 kb on - strand, within Pf6N2E2_2566 at 3024.081 kb on - strand, within Pf6N2E2_2566 at 3024.198 kb on + strand, within Pf6N2E2_2566 at 3024.198 kb on + strand, within Pf6N2E2_2566 at 3024.198 kb on + strand, within Pf6N2E2_2566 at 3024.198 kb on + strand, within Pf6N2E2_2566 at 3024.199 kb on - strand, within Pf6N2E2_2566 at 3024.521 kb on + strand, within Pf6N2E2_2566 at 3024.560 kb on + strand, within Pf6N2E2_2566 at 3024.560 kb on + strand, within Pf6N2E2_2566 at 3024.741 kb on + strand at 3024.741 kb on + strand at 3024.750 kb on + strand at 3024.752 kb on + strand at 3024.753 kb on - strand at 3024.753 kb on - strand at 3025.169 kb on - strand, within Pf6N2E2_2567 at 3025.169 kb on - strand, within Pf6N2E2_2567 at 3025.177 kb on + strand, within Pf6N2E2_2567 at 3025.177 kb on + strand, within Pf6N2E2_2567 at 3025.178 kb on - strand, within Pf6N2E2_2567 at 3025.178 kb on - strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.189 kb on + strand, within Pf6N2E2_2567 at 3025.500 kb on + strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.501 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.531 kb on - strand, within Pf6N2E2_2567 at 3025.561 kb on + strand at 3025.561 kb on + strand at 3025.575 kb on + strand at 3025.818 kb on + strand, within Pf6N2E2_2568 at 3025.818 kb on + strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.819 kb on - strand, within Pf6N2E2_2568 at 3025.886 kb on + strand, within Pf6N2E2_2568 at 3025.886 kb on + strand, within Pf6N2E2_2568 at 3025.887 kb on - strand, within Pf6N2E2_2568 at 3025.887 kb on - strand, within Pf6N2E2_2568 at 3025.992 kb on + strand, within Pf6N2E2_2568 at 3026.070 kb on + strand, within Pf6N2E2_2568 at 3026.108 kb on + strand, within Pf6N2E2_2568 at 3026.108 kb on + strand, within Pf6N2E2_2568 at 3026.109 kb on - strand, within Pf6N2E2_2568 at 3026.328 kb on + strand at 3026.329 kb on - strand at 3026.329 kb on - strand at 3026.415 kb on + strand at 3026.415 kb on + strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.484 kb on - strand at 3026.607 kb on - strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.631 kb on + strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569 at 3026.632 kb on - strand, within Pf6N2E2_2569
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,023,760 - Pf6N2E2_2565 0.88 -2.4 3,023,760 - Pf6N2E2_2565 0.88 -3.6 3,023,798 - -0.6 3,023,811 + -2.3 3,023,909 + -1.3 3,024,055 - Pf6N2E2_2566 0.19 -0.7 3,024,056 + Pf6N2E2_2566 0.19 -2.1 3,024,056 + Pf6N2E2_2566 0.19 -3.0 3,024,056 + Pf6N2E2_2566 0.19 -2.2 3,024,056 + Pf6N2E2_2566 0.19 +0.9 3,024,056 + Pf6N2E2_2566 0.19 -3.4 3,024,057 - Pf6N2E2_2566 0.19 -1.2 3,024,057 - Pf6N2E2_2566 0.19 -0.9 3,024,057 - Pf6N2E2_2566 0.19 +1.8 3,024,057 - Pf6N2E2_2566 0.19 -0.6 3,024,057 - Pf6N2E2_2566 0.19 -0.8 3,024,080 + Pf6N2E2_2566 0.22 -2.1 3,024,081 - Pf6N2E2_2566 0.22 -1.4 3,024,081 - Pf6N2E2_2566 0.22 -2.4 3,024,081 - Pf6N2E2_2566 0.22 -0.9 3,024,198 + Pf6N2E2_2566 0.37 -2.4 3,024,198 + Pf6N2E2_2566 0.37 -0.8 3,024,198 + Pf6N2E2_2566 0.37 -0.2 3,024,198 + Pf6N2E2_2566 0.37 -2.5 3,024,199 - Pf6N2E2_2566 0.37 -2.7 3,024,521 + Pf6N2E2_2566 0.77 -2.5 3,024,560 + Pf6N2E2_2566 0.82 +0.1 3,024,560 + Pf6N2E2_2566 0.82 -1.7 3,024,741 + -2.3 3,024,741 + -1.3 3,024,750 + -1.0 3,024,752 + -3.8 3,024,753 - +2.0 3,024,753 - -1.2 3,025,169 - Pf6N2E2_2567 0.47 -2.2 3,025,169 - Pf6N2E2_2567 0.47 -2.8 3,025,177 + Pf6N2E2_2567 0.48 -1.9 3,025,177 + Pf6N2E2_2567 0.48 -4.0 3,025,178 - Pf6N2E2_2567 0.48 -2.3 3,025,178 - Pf6N2E2_2567 0.48 -1.7 3,025,189 + Pf6N2E2_2567 0.50 -1.7 3,025,189 + Pf6N2E2_2567 0.50 -1.8 3,025,189 + Pf6N2E2_2567 0.50 -2.1 3,025,500 + Pf6N2E2_2567 0.85 -3.6 3,025,501 - Pf6N2E2_2567 0.85 -2.4 3,025,501 - Pf6N2E2_2567 0.85 -2.5 3,025,501 - Pf6N2E2_2567 0.85 -1.3 3,025,531 - Pf6N2E2_2567 0.88 +0.3 3,025,531 - Pf6N2E2_2567 0.88 -1.8 3,025,531 - Pf6N2E2_2567 0.88 +0.4 3,025,561 + -1.1 3,025,561 + -0.5 3,025,575 + -1.1 3,025,818 + Pf6N2E2_2568 0.23 -0.9 3,025,818 + Pf6N2E2_2568 0.23 -1.0 3,025,819 - Pf6N2E2_2568 0.23 -2.0 3,025,819 - Pf6N2E2_2568 0.23 -1.7 3,025,819 - Pf6N2E2_2568 0.23 -2.2 3,025,819 - Pf6N2E2_2568 0.23 -2.8 3,025,819 - Pf6N2E2_2568 0.23 -2.6 3,025,819 - Pf6N2E2_2568 0.23 -2.6 3,025,886 + Pf6N2E2_2568 0.32 -3.2 3,025,886 + Pf6N2E2_2568 0.32 -2.6 3,025,887 - Pf6N2E2_2568 0.32 -1.5 3,025,887 - Pf6N2E2_2568 0.32 -1.1 3,025,992 + Pf6N2E2_2568 0.46 -1.4 3,026,070 + Pf6N2E2_2568 0.57 -1.8 3,026,108 + Pf6N2E2_2568 0.62 -0.8 3,026,108 + Pf6N2E2_2568 0.62 +0.5 3,026,109 - Pf6N2E2_2568 0.62 -3.2 3,026,328 + -1.9 3,026,329 - -2.2 3,026,329 - -2.0 3,026,415 + -3.2 3,026,415 + -1.2 3,026,484 - -3.8 3,026,484 - -2.3 3,026,484 - -2.0 3,026,484 - -2.3 3,026,607 - Pf6N2E2_2569 0.19 -3.2 3,026,631 + Pf6N2E2_2569 0.21 -2.4 3,026,631 + Pf6N2E2_2569 0.21 -2.5 3,026,631 + Pf6N2E2_2569 0.21 -2.5 3,026,631 + Pf6N2E2_2569 0.21 -2.5 3,026,631 + Pf6N2E2_2569 0.21 -2.4 3,026,632 - Pf6N2E2_2569 0.21 -2.5 3,026,632 - Pf6N2E2_2569 0.21 -2.6 3,026,632 - Pf6N2E2_2569 0.21 -1.7 3,026,632 - Pf6N2E2_2569 0.21 -2.1
Or see this region's nucleotide sequence