Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotides Pf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotides Pf6N2E2_242 and Pf6N2E2_243 are separated by 49 nucleotides
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519
_241
Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202
_242
Pf6N2E2_243 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 303,252 to 304,157
_243
Position (kb) 302
303 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 301.423 kb on - strand, within Pf6N2E2_240 at 301.641 kb on + strand, within Pf6N2E2_240 at 301.642 kb on - strand, within Pf6N2E2_240 at 301.952 kb on + strand at 301.981 kb on + strand at 302.115 kb on - strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.244 kb on + strand at 302.336 kb on - strand at 302.481 kb on + strand, within Pf6N2E2_241 at 302.540 kb on + strand at 302.540 kb on + strand at 302.612 kb on + strand, within Pf6N2E2_242 at 302.677 kb on + strand, within Pf6N2E2_242 at 302.682 kb on - strand, within Pf6N2E2_242 at 302.840 kb on - strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.990 kb on - strand, within Pf6N2E2_242 at 303.196 kb on + strand at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.406 kb on - strand, within Pf6N2E2_243 at 303.519 kb on + strand, within Pf6N2E2_243
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 301,423 - Pf6N2E2_240 0.47 -1.5 301,641 + Pf6N2E2_240 0.72 -0.8 301,642 - Pf6N2E2_240 0.72 +0.3 301,952 + -0.9 301,981 + -1.1 302,115 - +1.2 302,157 + +0.5 302,157 + +1.2 302,157 + -1.0 302,244 + +0.5 302,336 - +1.5 302,481 + Pf6N2E2_241 0.73 -2.7 302,540 + +0.3 302,540 + -0.7 302,612 + Pf6N2E2_242 0.14 -0.8 302,677 + Pf6N2E2_242 0.23 -1.0 302,682 - Pf6N2E2_242 0.24 -0.6 302,840 - Pf6N2E2_242 0.47 -1.4 302,843 + Pf6N2E2_242 0.47 -0.3 302,843 + Pf6N2E2_242 0.47 -0.1 302,843 + Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 -0.2 302,843 + Pf6N2E2_242 0.47 -0.0 302,843 + Pf6N2E2_242 0.47 +0.8 302,843 + Pf6N2E2_242 0.47 -0.1 302,843 + Pf6N2E2_242 0.47 -0.7 302,844 - Pf6N2E2_242 0.48 +0.9 302,844 - Pf6N2E2_242 0.48 -0.3 302,989 + Pf6N2E2_242 0.69 -0.8 302,989 + Pf6N2E2_242 0.69 +0.1 302,989 + Pf6N2E2_242 0.69 +1.5 302,990 - Pf6N2E2_242 0.69 -1.3 303,196 + -1.7 303,405 + Pf6N2E2_243 0.17 -1.3 303,405 + Pf6N2E2_243 0.17 -0.5 303,405 + Pf6N2E2_243 0.17 +3.3 303,405 + Pf6N2E2_243 0.17 +0.1 303,406 - Pf6N2E2_243 0.17 +0.7 303,519 + Pf6N2E2_243 0.29 -0.5
Or see this region's nucleotide sequence