Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1741 and Pf6N2E2_1742 are separated by 187 nucleotides Pf6N2E2_1742 and Pf6N2E2_1743 overlap by 1 nucleotides
Pf6N2E2_1741 - Transketolase, N-terminal section (EC 2.2.1.1), at 2,053,695 to 2,054,579
_1741
Pf6N2E2_1742 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,054,767 to 2,056,158
_1742
Pf6N2E2_1743 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,056,158 to 2,057,264
_1743
Position (kb)
2054
2055
2056
2057 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2053.792 kb on + strand, within Pf6N2E2_1741 at 2053.793 kb on - strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.118 kb on + strand, within Pf6N2E2_1741 at 2054.119 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.401 kb on + strand, within Pf6N2E2_1741 at 2054.444 kb on - strand, within Pf6N2E2_1741 at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.927 kb on - strand, within Pf6N2E2_1742 at 2055.341 kb on - strand, within Pf6N2E2_1742 at 2055.678 kb on - strand, within Pf6N2E2_1742 at 2055.728 kb on + strand, within Pf6N2E2_1742 at 2055.729 kb on - strand, within Pf6N2E2_1742 at 2055.750 kb on + strand, within Pf6N2E2_1742 at 2055.805 kb on + strand, within Pf6N2E2_1742 at 2055.942 kb on - strand, within Pf6N2E2_1742 at 2056.227 kb on + strand at 2056.228 kb on - strand at 2056.444 kb on - strand, within Pf6N2E2_1743 at 2056.714 kb on - strand, within Pf6N2E2_1743 at 2056.758 kb on + strand, within Pf6N2E2_1743
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,053,792 + Pf6N2E2_1741 0.11 -0.3 2,053,793 - Pf6N2E2_1741 0.11 -1.0 2,054,107 + Pf6N2E2_1741 0.47 -1.6 2,054,107 + Pf6N2E2_1741 0.47 -1.1 2,054,118 + Pf6N2E2_1741 0.48 -0.7 2,054,119 - Pf6N2E2_1741 0.48 -0.3 2,054,399 - Pf6N2E2_1741 0.80 -1.6 2,054,399 - Pf6N2E2_1741 0.80 +0.2 2,054,399 - Pf6N2E2_1741 0.80 +0.3 2,054,401 + Pf6N2E2_1741 0.80 +1.7 2,054,444 - Pf6N2E2_1741 0.85 +4.6 2,054,899 + +0.5 2,054,899 + -2.9 2,054,899 + -0.0 2,054,899 + -1.5 2,054,899 + +1.3 2,054,900 - -0.9 2,054,900 - -2.3 2,054,900 - +0.8 2,054,900 - +0.1 2,054,926 + Pf6N2E2_1742 0.11 -0.6 2,054,926 + Pf6N2E2_1742 0.11 -0.1 2,054,927 - Pf6N2E2_1742 0.11 +1.5 2,055,341 - Pf6N2E2_1742 0.41 -0.4 2,055,678 - Pf6N2E2_1742 0.65 -1.2 2,055,728 + Pf6N2E2_1742 0.69 -0.8 2,055,729 - Pf6N2E2_1742 0.69 -1.0 2,055,750 + Pf6N2E2_1742 0.71 -0.9 2,055,805 + Pf6N2E2_1742 0.75 -2.0 2,055,942 - Pf6N2E2_1742 0.84 +0.2 2,056,227 + -0.0 2,056,228 - -0.1 2,056,444 - Pf6N2E2_1743 0.26 -0.2 2,056,714 - Pf6N2E2_1743 0.50 +0.0 2,056,758 + Pf6N2E2_1743 0.54 -0.3
Or see this region's nucleotide sequence