Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1570 and Pf6N2E2_1571 are separated by 10 nucleotides Pf6N2E2_1571 and Pf6N2E2_1572 are separated by 136 nucleotides
Pf6N2E2_1570 - Sll1359 protein, at 1,832,834 to 1,833,976
_1570
Pf6N2E2_1571 - FIG00966665: hypothetical protein, at 1,833,987 to 1,836,371
_1571
Pf6N2E2_1572 - hypothetical protein, at 1,836,508 to 1,837,428
_1572
Position (kb)
1833
1834
1835
1836
1837 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1833.077 kb on + strand, within Pf6N2E2_1570 at 1833.108 kb on - strand, within Pf6N2E2_1570 at 1833.108 kb on - strand, within Pf6N2E2_1570 at 1833.164 kb on - strand, within Pf6N2E2_1570 at 1833.187 kb on + strand, within Pf6N2E2_1570 at 1833.270 kb on + strand, within Pf6N2E2_1570 at 1833.308 kb on + strand, within Pf6N2E2_1570 at 1833.329 kb on + strand, within Pf6N2E2_1570 at 1833.461 kb on + strand, within Pf6N2E2_1570 at 1833.462 kb on - strand, within Pf6N2E2_1570 at 1833.476 kb on + strand, within Pf6N2E2_1570 at 1833.476 kb on + strand, within Pf6N2E2_1570 at 1833.477 kb on - strand, within Pf6N2E2_1570 at 1833.497 kb on - strand, within Pf6N2E2_1570 at 1833.500 kb on + strand, within Pf6N2E2_1570 at 1833.512 kb on + strand, within Pf6N2E2_1570 at 1833.513 kb on - strand, within Pf6N2E2_1570 at 1833.596 kb on - strand, within Pf6N2E2_1570 at 1833.596 kb on - strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.599 kb on + strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.600 kb on - strand, within Pf6N2E2_1570 at 1833.714 kb on - strand, within Pf6N2E2_1570 at 1833.754 kb on - strand, within Pf6N2E2_1570 at 1833.995 kb on - strand at 1834.011 kb on - strand at 1834.015 kb on - strand at 1834.015 kb on - strand at 1834.028 kb on + strand at 1834.029 kb on - strand at 1834.029 kb on - strand at 1834.029 kb on - strand at 1834.068 kb on + strand at 1834.078 kb on - strand at 1834.203 kb on + strand at 1834.204 kb on - strand at 1834.204 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.206 kb on - strand at 1834.216 kb on + strand at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.248 kb on + strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.249 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.303 kb on - strand, within Pf6N2E2_1571 at 1834.353 kb on + strand, within Pf6N2E2_1571 at 1834.354 kb on - strand, within Pf6N2E2_1571 at 1834.354 kb on - strand, within Pf6N2E2_1571 at 1834.479 kb on + strand at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.593 kb on + strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.594 kb on - strand, within Pf6N2E2_1571 at 1834.731 kb on + strand, within Pf6N2E2_1571 at 1834.732 kb on - strand, within Pf6N2E2_1571 at 1834.777 kb on - strand, within Pf6N2E2_1571 at 1834.777 kb on - strand, within Pf6N2E2_1571 at 1834.969 kb on + strand at 1835.033 kb on + strand, within Pf6N2E2_1571 at 1835.080 kb on - strand, within Pf6N2E2_1571 at 1835.137 kb on - strand, within Pf6N2E2_1571 at 1835.200 kb on - strand, within Pf6N2E2_1571 at 1835.201 kb on + strand, within Pf6N2E2_1571 at 1835.202 kb on - strand, within Pf6N2E2_1571 at 1835.202 kb on - strand, within Pf6N2E2_1571 at 1835.202 kb on - strand, within Pf6N2E2_1571 at 1835.272 kb on + strand, within Pf6N2E2_1571 at 1835.273 kb on - strand, within Pf6N2E2_1571 at 1835.485 kb on - strand, within Pf6N2E2_1571 at 1835.485 kb on - strand, within Pf6N2E2_1571 at 1835.485 kb on - strand, within Pf6N2E2_1571 at 1835.485 kb on - strand, within Pf6N2E2_1571 at 1835.506 kb on - strand, within Pf6N2E2_1571 at 1835.622 kb on + strand, within Pf6N2E2_1571 at 1835.623 kb on - strand, within Pf6N2E2_1571 at 1835.776 kb on - strand, within Pf6N2E2_1571 at 1835.823 kb on + strand, within Pf6N2E2_1571 at 1835.823 kb on + strand, within Pf6N2E2_1571 at 1835.824 kb on - strand, within Pf6N2E2_1571 at 1835.824 kb on - strand, within Pf6N2E2_1571 at 1835.829 kb on + strand, within Pf6N2E2_1571 at 1835.829 kb on + strand, within Pf6N2E2_1571 at 1835.829 kb on + strand, within Pf6N2E2_1571 at 1835.829 kb on + strand, within Pf6N2E2_1571 at 1835.829 kb on + strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.830 kb on - strand, within Pf6N2E2_1571 at 1835.973 kb on + strand, within Pf6N2E2_1571 at 1835.974 kb on - strand, within Pf6N2E2_1571 at 1835.974 kb on - strand, within Pf6N2E2_1571 at 1835.974 kb on - strand, within Pf6N2E2_1571 at 1835.974 kb on - strand, within Pf6N2E2_1571 at 1836.042 kb on + strand, within Pf6N2E2_1571 at 1836.042 kb on + strand, within Pf6N2E2_1571 at 1836.042 kb on + strand, within Pf6N2E2_1571 at 1836.043 kb on - strand, within Pf6N2E2_1571 at 1836.043 kb on - strand, within Pf6N2E2_1571 at 1836.058 kb on - strand, within Pf6N2E2_1571 at 1836.126 kb on + strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.127 kb on - strand, within Pf6N2E2_1571 at 1836.129 kb on + strand, within Pf6N2E2_1571 at 1836.129 kb on + strand, within Pf6N2E2_1571 at 1836.129 kb on + strand, within Pf6N2E2_1571 at 1836.129 kb on + strand, within Pf6N2E2_1571 at 1836.130 kb on - strand, within Pf6N2E2_1571 at 1836.136 kb on - strand at 1836.153 kb on + strand at 1836.153 kb on + strand at 1836.153 kb on + strand at 1836.154 kb on - strand at 1836.154 kb on - strand at 1836.154 kb on - strand at 1836.534 kb on - strand at 1836.534 kb on - strand at 1836.534 kb on - strand at 1836.593 kb on - strand at 1836.613 kb on - strand, within Pf6N2E2_1572 at 1836.722 kb on - strand, within Pf6N2E2_1572 at 1836.740 kb on - strand, within Pf6N2E2_1572 at 1836.797 kb on - strand, within Pf6N2E2_1572 at 1836.820 kb on + strand, within Pf6N2E2_1572 at 1836.820 kb on + strand, within Pf6N2E2_1572 at 1836.821 kb on - strand, within Pf6N2E2_1572 at 1836.824 kb on - strand, within Pf6N2E2_1572 at 1836.835 kb on + strand, within Pf6N2E2_1572 at 1836.835 kb on + strand, within Pf6N2E2_1572 at 1836.837 kb on + strand, within Pf6N2E2_1572 at 1836.838 kb on - strand, within Pf6N2E2_1572 at 1836.838 kb on - strand, within Pf6N2E2_1572 at 1836.881 kb on - strand, within Pf6N2E2_1572 at 1836.890 kb on - strand, within Pf6N2E2_1572 at 1836.926 kb on - strand, within Pf6N2E2_1572 at 1836.926 kb on - strand, within Pf6N2E2_1572 at 1836.926 kb on - strand, within Pf6N2E2_1572 at 1836.961 kb on + strand, within Pf6N2E2_1572 at 1836.961 kb on + strand, within Pf6N2E2_1572 at 1837.265 kb on - strand, within Pf6N2E2_1572
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,833,077 + Pf6N2E2_1570 0.21 -2.0 1,833,108 - Pf6N2E2_1570 0.24 +0.4 1,833,108 - Pf6N2E2_1570 0.24 +0.7 1,833,164 - Pf6N2E2_1570 0.29 -0.2 1,833,187 + Pf6N2E2_1570 0.31 -1.3 1,833,270 + Pf6N2E2_1570 0.38 -1.2 1,833,308 + Pf6N2E2_1570 0.41 +0.2 1,833,329 + Pf6N2E2_1570 0.43 +1.5 1,833,461 + Pf6N2E2_1570 0.55 -0.3 1,833,462 - Pf6N2E2_1570 0.55 -0.1 1,833,476 + Pf6N2E2_1570 0.56 -0.9 1,833,476 + Pf6N2E2_1570 0.56 -2.1 1,833,477 - Pf6N2E2_1570 0.56 +0.9 1,833,497 - Pf6N2E2_1570 0.58 +0.1 1,833,500 + Pf6N2E2_1570 0.58 -0.4 1,833,512 + Pf6N2E2_1570 0.59 +1.0 1,833,513 - Pf6N2E2_1570 0.59 -0.0 1,833,596 - Pf6N2E2_1570 0.67 -0.8 1,833,596 - Pf6N2E2_1570 0.67 -2.5 1,833,599 + Pf6N2E2_1570 0.67 -0.0 1,833,599 + Pf6N2E2_1570 0.67 -1.7 1,833,599 + Pf6N2E2_1570 0.67 -0.5 1,833,599 + Pf6N2E2_1570 0.67 -0.9 1,833,600 - Pf6N2E2_1570 0.67 -0.1 1,833,600 - Pf6N2E2_1570 0.67 +0.2 1,833,600 - Pf6N2E2_1570 0.67 +0.4 1,833,600 - Pf6N2E2_1570 0.67 -0.7 1,833,714 - Pf6N2E2_1570 0.77 +0.8 1,833,754 - Pf6N2E2_1570 0.80 -0.4 1,833,995 - +0.8 1,834,011 - -1.1 1,834,015 - +0.8 1,834,015 - -0.5 1,834,028 + +0.8 1,834,029 - -0.8 1,834,029 - -2.2 1,834,029 - -0.9 1,834,068 + -2.2 1,834,078 - -3.0 1,834,203 + -0.2 1,834,204 - -0.8 1,834,204 - -0.3 1,834,206 - -0.5 1,834,206 - -0.9 1,834,206 - -0.6 1,834,206 - -0.4 1,834,206 - -0.5 1,834,216 + -1.4 1,834,248 + Pf6N2E2_1571 0.11 +1.0 1,834,248 + Pf6N2E2_1571 0.11 +0.6 1,834,248 + Pf6N2E2_1571 0.11 +0.2 1,834,248 + Pf6N2E2_1571 0.11 -0.1 1,834,249 - Pf6N2E2_1571 0.11 -0.7 1,834,249 - Pf6N2E2_1571 0.11 -0.2 1,834,249 - Pf6N2E2_1571 0.11 -2.5 1,834,249 - Pf6N2E2_1571 0.11 +0.5 1,834,249 - Pf6N2E2_1571 0.11 +0.3 1,834,249 - Pf6N2E2_1571 0.11 +0.2 1,834,303 - Pf6N2E2_1571 0.13 -0.1 1,834,303 - Pf6N2E2_1571 0.13 -0.2 1,834,303 - Pf6N2E2_1571 0.13 -1.6 1,834,353 + Pf6N2E2_1571 0.15 -1.9 1,834,354 - Pf6N2E2_1571 0.15 -2.7 1,834,354 - Pf6N2E2_1571 0.15 -0.4 1,834,479 + +1.4 1,834,593 + Pf6N2E2_1571 0.25 +1.0 1,834,593 + Pf6N2E2_1571 0.25 -0.6 1,834,593 + Pf6N2E2_1571 0.25 +0.8 1,834,593 + Pf6N2E2_1571 0.25 +0.7 1,834,593 + Pf6N2E2_1571 0.25 -0.8 1,834,593 + Pf6N2E2_1571 0.25 +0.4 1,834,594 - Pf6N2E2_1571 0.25 -1.1 1,834,594 - Pf6N2E2_1571 0.25 -0.7 1,834,594 - Pf6N2E2_1571 0.25 +0.3 1,834,594 - Pf6N2E2_1571 0.25 -0.0 1,834,594 - Pf6N2E2_1571 0.25 +0.1 1,834,731 + Pf6N2E2_1571 0.31 -0.1 1,834,732 - Pf6N2E2_1571 0.31 +0.6 1,834,777 - Pf6N2E2_1571 0.33 +0.3 1,834,777 - Pf6N2E2_1571 0.33 +0.5 1,834,969 + +0.6 1,835,033 + Pf6N2E2_1571 0.44 -1.6 1,835,080 - Pf6N2E2_1571 0.46 +1.5 1,835,137 - Pf6N2E2_1571 0.48 -2.1 1,835,200 - Pf6N2E2_1571 0.51 -0.4 1,835,201 + Pf6N2E2_1571 0.51 +0.4 1,835,202 - Pf6N2E2_1571 0.51 -0.6 1,835,202 - Pf6N2E2_1571 0.51 +0.4 1,835,202 - Pf6N2E2_1571 0.51 -1.3 1,835,272 + Pf6N2E2_1571 0.54 -0.8 1,835,273 - Pf6N2E2_1571 0.54 +1.2 1,835,485 - Pf6N2E2_1571 0.63 +1.0 1,835,485 - Pf6N2E2_1571 0.63 -0.8 1,835,485 - Pf6N2E2_1571 0.63 +0.7 1,835,485 - Pf6N2E2_1571 0.63 -0.2 1,835,506 - Pf6N2E2_1571 0.64 +0.4 1,835,622 + Pf6N2E2_1571 0.69 -0.3 1,835,623 - Pf6N2E2_1571 0.69 +0.4 1,835,776 - Pf6N2E2_1571 0.75 +0.8 1,835,823 + Pf6N2E2_1571 0.77 -2.1 1,835,823 + Pf6N2E2_1571 0.77 -1.1 1,835,824 - Pf6N2E2_1571 0.77 +0.0 1,835,824 - Pf6N2E2_1571 0.77 +1.0 1,835,829 + Pf6N2E2_1571 0.77 +0.4 1,835,829 + Pf6N2E2_1571 0.77 -1.0 1,835,829 + Pf6N2E2_1571 0.77 -3.1 1,835,829 + Pf6N2E2_1571 0.77 -2.3 1,835,829 + Pf6N2E2_1571 0.77 -1.7 1,835,830 - Pf6N2E2_1571 0.77 -2.3 1,835,830 - Pf6N2E2_1571 0.77 -1.1 1,835,830 - Pf6N2E2_1571 0.77 -1.3 1,835,830 - Pf6N2E2_1571 0.77 -0.1 1,835,830 - Pf6N2E2_1571 0.77 -0.5 1,835,830 - Pf6N2E2_1571 0.77 -0.8 1,835,830 - Pf6N2E2_1571 0.77 +2.2 1,835,830 - Pf6N2E2_1571 0.77 +0.9 1,835,830 - Pf6N2E2_1571 0.77 -1.2 1,835,830 - Pf6N2E2_1571 0.77 -0.7 1,835,830 - Pf6N2E2_1571 0.77 -2.1 1,835,973 + Pf6N2E2_1571 0.83 -0.5 1,835,974 - Pf6N2E2_1571 0.83 -0.8 1,835,974 - Pf6N2E2_1571 0.83 -0.3 1,835,974 - Pf6N2E2_1571 0.83 -0.2 1,835,974 - Pf6N2E2_1571 0.83 +0.4 1,836,042 + Pf6N2E2_1571 0.86 -2.1 1,836,042 + Pf6N2E2_1571 0.86 -0.9 1,836,042 + Pf6N2E2_1571 0.86 -0.8 1,836,043 - Pf6N2E2_1571 0.86 +0.6 1,836,043 - Pf6N2E2_1571 0.86 -0.1 1,836,058 - Pf6N2E2_1571 0.87 -0.5 1,836,126 + Pf6N2E2_1571 0.90 -1.5 1,836,127 - Pf6N2E2_1571 0.90 +0.1 1,836,127 - Pf6N2E2_1571 0.90 -0.5 1,836,127 - Pf6N2E2_1571 0.90 -1.1 1,836,127 - Pf6N2E2_1571 0.90 -1.4 1,836,127 - Pf6N2E2_1571 0.90 -2.3 1,836,127 - Pf6N2E2_1571 0.90 +1.2 1,836,129 + Pf6N2E2_1571 0.90 +0.5 1,836,129 + Pf6N2E2_1571 0.90 -0.9 1,836,129 + Pf6N2E2_1571 0.90 -1.1 1,836,129 + Pf6N2E2_1571 0.90 +0.1 1,836,130 - Pf6N2E2_1571 0.90 -2.0 1,836,136 - -0.5 1,836,153 + -0.2 1,836,153 + -3.2 1,836,153 + +0.1 1,836,154 - +0.1 1,836,154 - -0.1 1,836,154 - -0.4 1,836,534 - +0.4 1,836,534 - -0.7 1,836,534 - -0.4 1,836,593 - -2.4 1,836,613 - Pf6N2E2_1572 0.11 +0.8 1,836,722 - Pf6N2E2_1572 0.23 +0.3 1,836,740 - Pf6N2E2_1572 0.25 -1.2 1,836,797 - Pf6N2E2_1572 0.31 +0.2 1,836,820 + Pf6N2E2_1572 0.34 +1.2 1,836,820 + Pf6N2E2_1572 0.34 -3.1 1,836,821 - Pf6N2E2_1572 0.34 +0.5 1,836,824 - Pf6N2E2_1572 0.34 -0.1 1,836,835 + Pf6N2E2_1572 0.36 +1.3 1,836,835 + Pf6N2E2_1572 0.36 -0.3 1,836,837 + Pf6N2E2_1572 0.36 -0.4 1,836,838 - Pf6N2E2_1572 0.36 +1.7 1,836,838 - Pf6N2E2_1572 0.36 -0.9 1,836,881 - Pf6N2E2_1572 0.40 +0.6 1,836,890 - Pf6N2E2_1572 0.41 -1.4 1,836,926 - Pf6N2E2_1572 0.45 -0.5 1,836,926 - Pf6N2E2_1572 0.45 -1.5 1,836,926 - Pf6N2E2_1572 0.45 -1.1 1,836,961 + Pf6N2E2_1572 0.49 +1.4 1,836,961 + Pf6N2E2_1572 0.49 +1.2 1,837,265 - Pf6N2E2_1572 0.82 +0.0
Or see this region's nucleotide sequence