Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1568

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1566 and Pf6N2E2_1567 are separated by 39 nucleotidesPf6N2E2_1567 and Pf6N2E2_1568 overlap by 4 nucleotidesPf6N2E2_1568 and Pf6N2E2_1569 overlap by 11 nucleotides Pf6N2E2_1566 - FIG00960770: hypothetical protein, at 1,828,497 to 1,829,270 _1566 Pf6N2E2_1567 - FIG00964846: hypothetical protein, at 1,829,310 to 1,830,194 _1567 Pf6N2E2_1568 - FIG00960890: hypothetical protein, at 1,830,191 to 1,831,420 _1568 Pf6N2E2_1569 - FIG00957800: hypothetical protein, at 1,831,410 to 1,832,855 _1569 Position (kb) 1830 1831 1832Strain fitness (log2 ratio) -2 -1 0 1at 1829.225 kb on - strandat 1829.319 kb on + strandat 1829.457 kb on + strand, within Pf6N2E2_1567at 1829.457 kb on + strand, within Pf6N2E2_1567at 1829.457 kb on + strand, within Pf6N2E2_1567at 1829.457 kb on + strand, within Pf6N2E2_1567at 1829.743 kb on + strand, within Pf6N2E2_1567at 1829.743 kb on + strand, within Pf6N2E2_1567at 1829.743 kb on + strand, within Pf6N2E2_1567at 1829.744 kb on - strand, within Pf6N2E2_1567at 1829.744 kb on - strand, within Pf6N2E2_1567at 1829.744 kb on - strand, within Pf6N2E2_1567at 1830.231 kb on - strandat 1830.494 kb on + strand, within Pf6N2E2_1568at 1830.495 kb on - strand, within Pf6N2E2_1568at 1830.495 kb on - strand, within Pf6N2E2_1568at 1830.531 kb on - strand, within Pf6N2E2_1568at 1830.531 kb on - strand, within Pf6N2E2_1568at 1830.873 kb on - strand, within Pf6N2E2_1568at 1830.873 kb on - strand, within Pf6N2E2_1568at 1830.873 kb on - strand, within Pf6N2E2_1568at 1831.016 kb on + strand, within Pf6N2E2_1568at 1831.017 kb on - strand, within Pf6N2E2_1568at 1831.017 kb on - strand, within Pf6N2E2_1568at 1831.017 kb on - strand, within Pf6N2E2_1568at 1831.266 kb on - strand, within Pf6N2E2_1568at 1831.380 kb on + strandat 1831.380 kb on + strandat 1831.381 kb on - strandat 1831.651 kb on + strand, within Pf6N2E2_1569at 1831.652 kb on - strand, within Pf6N2E2_1569at 1831.755 kb on + strand, within Pf6N2E2_1569at 1831.755 kb on + strand, within Pf6N2E2_1569at 1831.755 kb on + strand, within Pf6N2E2_1569at 1831.755 kb on + strand, within Pf6N2E2_1569at 1831.756 kb on - strand, within Pf6N2E2_1569at 1831.756 kb on - strand, within Pf6N2E2_1569at 1831.764 kb on + strand, within Pf6N2E2_1569at 1831.764 kb on + strand, within Pf6N2E2_1569at 1831.764 kb on + strand, within Pf6N2E2_1569at 1831.764 kb on + strand, within Pf6N2E2_1569at 1831.765 kb on - strand, within Pf6N2E2_1569at 1831.765 kb on - strand, within Pf6N2E2_1569at 1831.765 kb on - strand, within Pf6N2E2_1569at 1831.765 kb on - strand, within Pf6N2E2_1569at 1831.836 kb on + strand, within Pf6N2E2_1569at 1831.836 kb on + strand, within Pf6N2E2_1569at 1831.837 kb on - strand, within Pf6N2E2_1569at 1831.998 kb on + strand, within Pf6N2E2_1569at 1831.998 kb on + strand, within Pf6N2E2_1569at 1832.065 kb on + strand, within Pf6N2E2_1569at 1832.298 kb on + strand, within Pf6N2E2_1569at 1832.298 kb on + strand, within Pf6N2E2_1569at 1832.298 kb on + strand, within Pf6N2E2_1569at 1832.299 kb on - strand, within Pf6N2E2_1569at 1832.299 kb on - strand, within Pf6N2E2_1569at 1832.299 kb on - strand, within Pf6N2E2_1569at 1832.299 kb on - strand, within Pf6N2E2_1569at 1832.375 kb on + strand, within Pf6N2E2_1569

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,829,225 - -0.0
1,829,319 + +0.2
1,829,457 + Pf6N2E2_1567 0.17 -0.5
1,829,457 + Pf6N2E2_1567 0.17 -0.7
1,829,457 + Pf6N2E2_1567 0.17 +1.2
1,829,457 + Pf6N2E2_1567 0.17 -1.1
1,829,743 + Pf6N2E2_1567 0.49 -0.0
1,829,743 + Pf6N2E2_1567 0.49 -1.3
1,829,743 + Pf6N2E2_1567 0.49 -1.8
1,829,744 - Pf6N2E2_1567 0.49 -1.1
1,829,744 - Pf6N2E2_1567 0.49 -0.2
1,829,744 - Pf6N2E2_1567 0.49 -0.1
1,830,231 - -0.1
1,830,494 + Pf6N2E2_1568 0.25 -1.4
1,830,495 - Pf6N2E2_1568 0.25 +0.1
1,830,495 - Pf6N2E2_1568 0.25 -0.2
1,830,531 - Pf6N2E2_1568 0.28 -2.3
1,830,531 - Pf6N2E2_1568 0.28 -2.4
1,830,873 - Pf6N2E2_1568 0.55 +0.2
1,830,873 - Pf6N2E2_1568 0.55 -0.6
1,830,873 - Pf6N2E2_1568 0.55 -1.3
1,831,016 + Pf6N2E2_1568 0.67 -1.2
1,831,017 - Pf6N2E2_1568 0.67 -0.3
1,831,017 - Pf6N2E2_1568 0.67 -2.3
1,831,017 - Pf6N2E2_1568 0.67 +0.5
1,831,266 - Pf6N2E2_1568 0.87 +0.3
1,831,380 + +1.4
1,831,380 + +0.3
1,831,381 - +0.1
1,831,651 + Pf6N2E2_1569 0.17 -0.7
1,831,652 - Pf6N2E2_1569 0.17 -1.1
1,831,755 + Pf6N2E2_1569 0.24 -0.4
1,831,755 + Pf6N2E2_1569 0.24 +0.5
1,831,755 + Pf6N2E2_1569 0.24 -0.3
1,831,755 + Pf6N2E2_1569 0.24 -0.2
1,831,756 - Pf6N2E2_1569 0.24 -1.7
1,831,756 - Pf6N2E2_1569 0.24 -2.0
1,831,764 + Pf6N2E2_1569 0.24 -2.3
1,831,764 + Pf6N2E2_1569 0.24 +0.4
1,831,764 + Pf6N2E2_1569 0.24 +0.2
1,831,764 + Pf6N2E2_1569 0.24 -0.6
1,831,765 - Pf6N2E2_1569 0.25 -2.0
1,831,765 - Pf6N2E2_1569 0.25 -0.7
1,831,765 - Pf6N2E2_1569 0.25 +0.4
1,831,765 - Pf6N2E2_1569 0.25 -2.1
1,831,836 + Pf6N2E2_1569 0.29 +1.5
1,831,836 + Pf6N2E2_1569 0.29 -0.4
1,831,837 - Pf6N2E2_1569 0.30 -0.6
1,831,998 + Pf6N2E2_1569 0.41 -0.5
1,831,998 + Pf6N2E2_1569 0.41 -1.9
1,832,065 + Pf6N2E2_1569 0.45 -2.7
1,832,298 + Pf6N2E2_1569 0.61 -1.4
1,832,298 + Pf6N2E2_1569 0.61 -1.6
1,832,298 + Pf6N2E2_1569 0.61 -0.9
1,832,299 - Pf6N2E2_1569 0.61 +1.1
1,832,299 - Pf6N2E2_1569 0.61 -0.8
1,832,299 - Pf6N2E2_1569 0.61 +0.2
1,832,299 - Pf6N2E2_1569 0.61 -1.0
1,832,375 + Pf6N2E2_1569 0.67 -1.3

Or see this region's nucleotide sequence