Experiment: PYE with Thallium(I) acetate 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03187 and CCNA_03188 are separated by 214 nucleotides CCNA_03188 and CCNA_03189 are separated by 24 nucleotides CCNA_03189 and CCNA_03190 overlap by 4 nucleotides
CCNA_03187: CCNA_03187 - cysteine/acetylserine efflux protein, at 3,347,646 to 3,348,599
_03187
CCNA_03188: CCNA_03188 - epoxide hydrolase, at 3,348,814 to 3,349,953
_03188
CCNA_03189: CCNA_03189 - glutathione-dependent formaldehyde-activating enzyme, at 3,349,978 to 3,350,382
_03189
CCNA_03190: CCNA_03190 - low-specificity L-threonine aldolase, at 3,350,379 to 3,351,413
_03190
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3347.879 kb on + strand, within CCNA_03187 at 3347.917 kb on + strand, within CCNA_03187 at 3347.947 kb on - strand, within CCNA_03187 at 3347.955 kb on + strand, within CCNA_03187 at 3347.977 kb on - strand, within CCNA_03187 at 3347.992 kb on + strand, within CCNA_03187 at 3348.014 kb on + strand, within CCNA_03187 at 3348.014 kb on + strand, within CCNA_03187 at 3348.022 kb on - strand, within CCNA_03187 at 3348.040 kb on + strand, within CCNA_03187 at 3348.045 kb on + strand, within CCNA_03187 at 3348.052 kb on + strand, within CCNA_03187 at 3348.067 kb on - strand, within CCNA_03187 at 3348.100 kb on + strand, within CCNA_03187 at 3348.105 kb on + strand, within CCNA_03187 at 3348.105 kb on + strand, within CCNA_03187 at 3348.144 kb on - strand, within CCNA_03187 at 3348.197 kb on + strand, within CCNA_03187 at 3348.218 kb on + strand, within CCNA_03187 at 3348.227 kb on + strand, within CCNA_03187 at 3348.227 kb on + strand, within CCNA_03187 at 3348.235 kb on - strand, within CCNA_03187 at 3348.236 kb on - strand, within CCNA_03187 at 3348.323 kb on + strand, within CCNA_03187 at 3348.323 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.324 kb on + strand, within CCNA_03187 at 3348.331 kb on - strand, within CCNA_03187 at 3348.332 kb on - strand, within CCNA_03187 at 3348.332 kb on - strand, within CCNA_03187 at 3348.347 kb on - strand, within CCNA_03187 at 3348.355 kb on + strand, within CCNA_03187 at 3348.369 kb on + strand, within CCNA_03187 at 3348.384 kb on + strand, within CCNA_03187 at 3348.384 kb on + strand, within CCNA_03187 at 3348.391 kb on - strand, within CCNA_03187 at 3348.392 kb on - strand, within CCNA_03187 at 3348.392 kb on - strand, within CCNA_03187 at 3348.440 kb on + strand, within CCNA_03187 at 3348.448 kb on - strand, within CCNA_03187 at 3348.458 kb on - strand, within CCNA_03187 at 3348.466 kb on + strand, within CCNA_03187 at 3348.474 kb on - strand, within CCNA_03187 at 3348.474 kb on - strand, within CCNA_03187 at 3348.490 kb on - strand, within CCNA_03187 at 3348.499 kb on + strand, within CCNA_03187 at 3348.563 kb on + strand at 3348.593 kb on + strand at 3348.593 kb on + strand at 3348.601 kb on - strand at 3348.601 kb on - strand at 3348.601 kb on - strand at 3348.601 kb on - strand at 3348.663 kb on - strand at 3348.756 kb on + strand at 3348.756 kb on + strand at 3348.756 kb on + strand at 3348.756 kb on + strand at 3348.764 kb on - strand at 3348.764 kb on - strand at 3348.764 kb on - strand at 3348.764 kb on - strand at 3348.815 kb on + strand at 3348.842 kb on + strand at 3348.865 kb on - strand at 3348.870 kb on - strand at 3348.888 kb on + strand at 3348.888 kb on - strand at 3348.902 kb on - strand at 3348.919 kb on + strand at 3348.919 kb on + strand at 3348.922 kb on - strand at 3348.927 kb on - strand at 3348.927 kb on - strand at 3348.927 kb on - strand at 3348.927 kb on - strand at 3348.927 kb on - strand at 3348.941 kb on + strand, within CCNA_03188 at 3348.951 kb on + strand, within CCNA_03188 at 3348.959 kb on - strand, within CCNA_03188 at 3348.959 kb on - strand, within CCNA_03188 at 3348.982 kb on - strand, within CCNA_03188 at 3348.982 kb on - strand, within CCNA_03188 at 3348.991 kb on + strand, within CCNA_03188 at 3348.991 kb on + strand, within CCNA_03188 at 3348.991 kb on + strand, within CCNA_03188 at 3348.999 kb on - strand, within CCNA_03188 at 3348.999 kb on - strand, within CCNA_03188 at 3349.041 kb on + strand, within CCNA_03188 at 3349.095 kb on + strand, within CCNA_03188 at 3349.095 kb on + strand, within CCNA_03188 at 3349.120 kb on - strand, within CCNA_03188 at 3349.125 kb on - strand, within CCNA_03188 at 3349.150 kb on + strand, within CCNA_03188 at 3349.158 kb on - strand, within CCNA_03188 at 3349.158 kb on - strand, within CCNA_03188 at 3349.160 kb on + strand, within CCNA_03188 at 3349.164 kb on - strand, within CCNA_03188 at 3349.230 kb on - strand, within CCNA_03188 at 3349.281 kb on + strand, within CCNA_03188 at 3349.284 kb on - strand, within CCNA_03188 at 3349.289 kb on + strand, within CCNA_03188 at 3349.289 kb on - strand, within CCNA_03188 at 3349.289 kb on - strand, within CCNA_03188 at 3349.325 kb on - strand, within CCNA_03188 at 3349.327 kb on + strand, within CCNA_03188 at 3349.358 kb on + strand, within CCNA_03188 at 3349.358 kb on + strand, within CCNA_03188 at 3349.366 kb on - strand, within CCNA_03188 at 3349.374 kb on + strand, within CCNA_03188 at 3349.378 kb on - strand, within CCNA_03188 at 3349.382 kb on - strand, within CCNA_03188 at 3349.395 kb on + strand, within CCNA_03188 at 3349.410 kb on - strand, within CCNA_03188 at 3349.430 kb on - strand, within CCNA_03188 at 3349.435 kb on - strand, within CCNA_03188 at 3349.453 kb on + strand, within CCNA_03188 at 3349.484 kb on + strand, within CCNA_03188 at 3349.489 kb on + strand, within CCNA_03188 at 3349.489 kb on + strand, within CCNA_03188 at 3349.576 kb on + strand, within CCNA_03188 at 3349.607 kb on - strand, within CCNA_03188 at 3349.625 kb on - strand, within CCNA_03188 at 3349.633 kb on - strand, within CCNA_03188 at 3349.636 kb on + strand, within CCNA_03188 at 3349.673 kb on + strand, within CCNA_03188 at 3349.729 kb on - strand, within CCNA_03188 at 3349.742 kb on - strand, within CCNA_03188 at 3349.814 kb on - strand, within CCNA_03188 at 3349.816 kb on + strand, within CCNA_03188 at 3349.816 kb on + strand, within CCNA_03188 at 3349.824 kb on - strand, within CCNA_03188 at 3349.843 kb on + strand at 3349.850 kb on - strand at 3349.888 kb on - strand at 3349.890 kb on - strand at 3349.904 kb on + strand at 3349.905 kb on - strand at 3349.947 kb on + strand at 3349.955 kb on - strand at 3350.068 kb on + strand, within CCNA_03189 at 3350.076 kb on - strand, within CCNA_03189 at 3350.090 kb on + strand, within CCNA_03189 at 3350.090 kb on + strand, within CCNA_03189 at 3350.102 kb on - strand, within CCNA_03189 at 3350.125 kb on - strand, within CCNA_03189 at 3350.136 kb on - strand, within CCNA_03189 at 3350.141 kb on - strand, within CCNA_03189 at 3350.177 kb on + strand, within CCNA_03189 at 3350.214 kb on - strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.218 kb on + strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.226 kb on - strand, within CCNA_03189 at 3350.232 kb on - strand, within CCNA_03189 at 3350.244 kb on - strand, within CCNA_03189 at 3350.267 kb on + strand, within CCNA_03189 at 3350.271 kb on + strand, within CCNA_03189 at 3350.305 kb on + strand, within CCNA_03189 at 3350.318 kb on + strand, within CCNA_03189 at 3350.344 kb on - strand at 3350.350 kb on + strand at 3350.437 kb on + strand at 3350.470 kb on + strand at 3350.481 kb on + strand at 3350.489 kb on - strand, within CCNA_03190 at 3350.502 kb on + strand, within CCNA_03190 at 3350.507 kb on + strand, within CCNA_03190 at 3350.536 kb on - strand, within CCNA_03190 at 3350.538 kb on + strand, within CCNA_03190 at 3350.546 kb on - strand, within CCNA_03190 at 3350.546 kb on - strand, within CCNA_03190 at 3350.546 kb on - strand, within CCNA_03190 at 3350.546 kb on - strand, within CCNA_03190 at 3350.558 kb on + strand, within CCNA_03190 at 3350.564 kb on + strand, within CCNA_03190 at 3350.567 kb on - strand, within CCNA_03190 at 3350.593 kb on - strand, within CCNA_03190 at 3350.598 kb on + strand, within CCNA_03190 at 3350.606 kb on - strand, within CCNA_03190 at 3350.626 kb on - strand, within CCNA_03190 at 3350.649 kb on + strand, within CCNA_03190 at 3350.654 kb on + strand, within CCNA_03190 at 3350.657 kb on - strand, within CCNA_03190 at 3350.662 kb on - strand, within CCNA_03190 at 3350.686 kb on + strand, within CCNA_03190 at 3350.686 kb on + strand, within CCNA_03190 at 3350.694 kb on - strand, within CCNA_03190 at 3350.694 kb on - strand, within CCNA_03190 at 3350.756 kb on - strand, within CCNA_03190 at 3350.828 kb on + strand, within CCNA_03190 at 3350.842 kb on + strand, within CCNA_03190 at 3350.861 kb on + strand, within CCNA_03190 at 3350.863 kb on - strand, within CCNA_03190 at 3350.882 kb on + strand, within CCNA_03190 at 3350.884 kb on - strand, within CCNA_03190 at 3350.884 kb on - strand, within CCNA_03190 at 3350.888 kb on + strand, within CCNA_03190 at 3350.888 kb on + strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.896 kb on - strand, within CCNA_03190 at 3350.909 kb on - strand, within CCNA_03190 at 3350.909 kb on - strand, within CCNA_03190 at 3350.909 kb on - strand, within CCNA_03190 at 3350.909 kb on - strand, within CCNA_03190 at 3350.941 kb on + strand, within CCNA_03190
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Thallium(I) acetate 0.004 mg/ml remove 3,347,879 + CCNA_03187 0.24 +2.5 3,347,917 + CCNA_03187 0.28 +0.3 3,347,947 - CCNA_03187 0.32 -0.6 3,347,955 + CCNA_03187 0.32 +0.1 3,347,977 - CCNA_03187 0.35 -1.3 3,347,992 + CCNA_03187 0.36 -0.5 3,348,014 + CCNA_03187 0.39 +0.2 3,348,014 + CCNA_03187 0.39 -1.7 3,348,022 - CCNA_03187 0.39 -0.8 3,348,040 + CCNA_03187 0.41 +0.9 3,348,045 + CCNA_03187 0.42 -1.1 3,348,052 + CCNA_03187 0.43 +0.2 3,348,067 - CCNA_03187 0.44 +0.6 3,348,100 + CCNA_03187 0.48 -0.2 3,348,105 + CCNA_03187 0.48 -0.2 3,348,105 + CCNA_03187 0.48 -0.1 3,348,144 - CCNA_03187 0.52 -0.3 3,348,197 + CCNA_03187 0.58 +1.0 3,348,218 + CCNA_03187 0.60 -0.4 3,348,227 + CCNA_03187 0.61 -0.3 3,348,227 + CCNA_03187 0.61 -0.1 3,348,235 - CCNA_03187 0.62 +1.6 3,348,236 - CCNA_03187 0.62 +0.7 3,348,323 + CCNA_03187 0.71 -0.6 3,348,323 + CCNA_03187 0.71 -0.9 3,348,324 + CCNA_03187 0.71 -1.1 3,348,324 + CCNA_03187 0.71 +0.4 3,348,324 + CCNA_03187 0.71 +1.1 3,348,324 + CCNA_03187 0.71 +0.4 3,348,324 + CCNA_03187 0.71 -0.3 3,348,324 + CCNA_03187 0.71 -0.9 3,348,324 + CCNA_03187 0.71 +0.4 3,348,331 - CCNA_03187 0.72 -0.4 3,348,332 - CCNA_03187 0.72 -1.8 3,348,332 - CCNA_03187 0.72 +0.5 3,348,347 - CCNA_03187 0.73 -1.2 3,348,355 + CCNA_03187 0.74 -1.9 3,348,369 + CCNA_03187 0.76 +1.1 3,348,384 + CCNA_03187 0.77 +0.1 3,348,384 + CCNA_03187 0.77 +0.2 3,348,391 - CCNA_03187 0.78 -1.3 3,348,392 - CCNA_03187 0.78 -0.1 3,348,392 - CCNA_03187 0.78 -0.9 3,348,440 + CCNA_03187 0.83 +0.1 3,348,448 - CCNA_03187 0.84 +0.2 3,348,458 - CCNA_03187 0.85 +2.0 3,348,466 + CCNA_03187 0.86 +0.6 3,348,474 - CCNA_03187 0.87 -0.4 3,348,474 - CCNA_03187 0.87 +0.4 3,348,490 - CCNA_03187 0.88 -2.0 3,348,499 + CCNA_03187 0.89 +0.7 3,348,563 + +0.9 3,348,593 + -0.8 3,348,593 + -1.2 3,348,601 - -1.8 3,348,601 - +0.6 3,348,601 - +2.9 3,348,601 - -0.5 3,348,663 - -0.2 3,348,756 + +0.6 3,348,756 + +0.7 3,348,756 + +0.1 3,348,756 + +1.9 3,348,764 - -1.5 3,348,764 - +0.8 3,348,764 - -1.3 3,348,764 - +0.9 3,348,815 + -0.8 3,348,842 + -0.2 3,348,865 - +0.4 3,348,870 - -0.7 3,348,888 + +0.7 3,348,888 - -0.9 3,348,902 - -0.7 3,348,919 + +0.7 3,348,919 + -0.2 3,348,922 - -1.8 3,348,927 - -0.2 3,348,927 - +0.4 3,348,927 - +0.7 3,348,927 - -1.4 3,348,927 - -0.6 3,348,941 + CCNA_03188 0.11 -0.9 3,348,951 + CCNA_03188 0.12 -0.4 3,348,959 - CCNA_03188 0.13 -1.2 3,348,959 - CCNA_03188 0.13 +0.9 3,348,982 - CCNA_03188 0.15 -0.6 3,348,982 - CCNA_03188 0.15 +1.0 3,348,991 + CCNA_03188 0.16 -1.1 3,348,991 + CCNA_03188 0.16 +0.9 3,348,991 + CCNA_03188 0.16 -0.4 3,348,999 - CCNA_03188 0.16 +0.4 3,348,999 - CCNA_03188 0.16 -2.0 3,349,041 + CCNA_03188 0.20 -0.0 3,349,095 + CCNA_03188 0.25 -1.4 3,349,095 + CCNA_03188 0.25 -1.0 3,349,120 - CCNA_03188 0.27 -0.8 3,349,125 - CCNA_03188 0.27 -2.2 3,349,150 + CCNA_03188 0.29 -0.7 3,349,158 - CCNA_03188 0.30 +0.2 3,349,158 - CCNA_03188 0.30 -0.3 3,349,160 + CCNA_03188 0.30 -0.6 3,349,164 - CCNA_03188 0.31 +1.3 3,349,230 - CCNA_03188 0.36 -2.2 3,349,281 + CCNA_03188 0.41 +1.6 3,349,284 - CCNA_03188 0.41 -1.2 3,349,289 + CCNA_03188 0.42 +0.2 3,349,289 - CCNA_03188 0.42 +0.1 3,349,289 - CCNA_03188 0.42 +1.4 3,349,325 - CCNA_03188 0.45 +0.0 3,349,327 + CCNA_03188 0.45 +0.4 3,349,358 + CCNA_03188 0.48 +0.4 3,349,358 + CCNA_03188 0.48 +1.9 3,349,366 - CCNA_03188 0.48 +0.1 3,349,374 + CCNA_03188 0.49 +0.3 3,349,378 - CCNA_03188 0.49 -0.1 3,349,382 - CCNA_03188 0.50 +0.7 3,349,395 + CCNA_03188 0.51 -0.9 3,349,410 - CCNA_03188 0.52 -1.2 3,349,430 - CCNA_03188 0.54 -2.5 3,349,435 - CCNA_03188 0.54 -0.4 3,349,453 + CCNA_03188 0.56 +0.2 3,349,484 + CCNA_03188 0.59 -0.4 3,349,489 + CCNA_03188 0.59 -1.0 3,349,489 + CCNA_03188 0.59 -0.5 3,349,576 + CCNA_03188 0.67 +0.1 3,349,607 - CCNA_03188 0.70 -1.0 3,349,625 - CCNA_03188 0.71 -1.5 3,349,633 - CCNA_03188 0.72 -1.8 3,349,636 + CCNA_03188 0.72 +1.5 3,349,673 + CCNA_03188 0.75 -1.0 3,349,729 - CCNA_03188 0.80 -0.2 3,349,742 - CCNA_03188 0.81 -2.2 3,349,814 - CCNA_03188 0.88 +0.1 3,349,816 + CCNA_03188 0.88 +0.9 3,349,816 + CCNA_03188 0.88 +0.0 3,349,824 - CCNA_03188 0.89 -1.5 3,349,843 + -2.0 3,349,850 - +0.3 3,349,888 - -1.5 3,349,890 - -1.1 3,349,904 + +0.7 3,349,905 - +0.1 3,349,947 + -0.3 3,349,955 - +0.0 3,350,068 + CCNA_03189 0.22 +2.9 3,350,076 - CCNA_03189 0.24 -0.9 3,350,090 + CCNA_03189 0.28 +0.9 3,350,090 + CCNA_03189 0.28 -1.2 3,350,102 - CCNA_03189 0.31 +0.6 3,350,125 - CCNA_03189 0.36 -0.1 3,350,136 - CCNA_03189 0.39 +1.3 3,350,141 - CCNA_03189 0.40 +0.6 3,350,177 + CCNA_03189 0.49 -1.6 3,350,214 - CCNA_03189 0.58 +1.6 3,350,218 + CCNA_03189 0.59 +2.1 3,350,218 + CCNA_03189 0.59 -0.3 3,350,218 + CCNA_03189 0.59 +1.3 3,350,218 + CCNA_03189 0.59 -1.7 3,350,218 + CCNA_03189 0.59 +0.4 3,350,218 + CCNA_03189 0.59 -0.6 3,350,218 + CCNA_03189 0.59 +0.9 3,350,226 - CCNA_03189 0.61 +1.1 3,350,226 - CCNA_03189 0.61 +0.5 3,350,226 - CCNA_03189 0.61 -1.7 3,350,226 - CCNA_03189 0.61 +0.2 3,350,226 - CCNA_03189 0.61 +1.3 3,350,226 - CCNA_03189 0.61 +0.3 3,350,226 - CCNA_03189 0.61 +0.3 3,350,226 - CCNA_03189 0.61 -0.2 3,350,232 - CCNA_03189 0.63 -1.0 3,350,244 - CCNA_03189 0.66 +0.4 3,350,267 + CCNA_03189 0.71 -0.8 3,350,271 + CCNA_03189 0.72 +1.3 3,350,305 + CCNA_03189 0.81 +0.9 3,350,318 + CCNA_03189 0.84 -0.2 3,350,344 - -0.1 3,350,350 + -1.1 3,350,437 + -0.3 3,350,470 + -0.7 3,350,481 + +0.9 3,350,489 - CCNA_03190 0.11 +1.3 3,350,502 + CCNA_03190 0.12 +1.3 3,350,507 + CCNA_03190 0.12 -1.9 3,350,536 - CCNA_03190 0.15 -1.2 3,350,538 + CCNA_03190 0.15 +0.9 3,350,546 - CCNA_03190 0.16 -0.2 3,350,546 - CCNA_03190 0.16 +0.4 3,350,546 - CCNA_03190 0.16 +1.6 3,350,546 - CCNA_03190 0.16 +0.4 3,350,558 + CCNA_03190 0.17 +1.3 3,350,564 + CCNA_03190 0.18 -0.2 3,350,567 - CCNA_03190 0.18 -0.7 3,350,593 - CCNA_03190 0.21 -0.3 3,350,598 + CCNA_03190 0.21 +0.4 3,350,606 - CCNA_03190 0.22 -0.9 3,350,626 - CCNA_03190 0.24 -0.7 3,350,649 + CCNA_03190 0.26 -0.4 3,350,654 + CCNA_03190 0.27 -0.0 3,350,657 - CCNA_03190 0.27 -0.9 3,350,662 - CCNA_03190 0.27 +1.6 3,350,686 + CCNA_03190 0.30 +0.4 3,350,686 + CCNA_03190 0.30 -0.6 3,350,694 - CCNA_03190 0.30 -1.5 3,350,694 - CCNA_03190 0.30 +0.3 3,350,756 - CCNA_03190 0.36 -1.2 3,350,828 + CCNA_03190 0.43 -0.8 3,350,842 + CCNA_03190 0.45 +0.3 3,350,861 + CCNA_03190 0.47 -0.1 3,350,863 - CCNA_03190 0.47 -0.3 3,350,882 + CCNA_03190 0.49 -1.1 3,350,884 - CCNA_03190 0.49 +0.9 3,350,884 - CCNA_03190 0.49 -1.4 3,350,888 + CCNA_03190 0.49 -2.2 3,350,888 + CCNA_03190 0.49 -1.5 3,350,896 - CCNA_03190 0.50 +0.3 3,350,896 - CCNA_03190 0.50 +1.2 3,350,896 - CCNA_03190 0.50 -0.8 3,350,896 - CCNA_03190 0.50 +0.2 3,350,896 - CCNA_03190 0.50 +0.1 3,350,896 - CCNA_03190 0.50 +1.1 3,350,909 - CCNA_03190 0.51 +0.6 3,350,909 - CCNA_03190 0.51 +0.7 3,350,909 - CCNA_03190 0.51 -1.0 3,350,909 - CCNA_03190 0.51 -0.3 3,350,941 + CCNA_03190 0.54 -0.1
Or see this region's nucleotide sequence