Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS20225 and ABZR86_RS20230 overlap by 1 nucleotides ABZR86_RS20230 and ABZR86_RS20235 are separated by 97 nucleotides ABZR86_RS20235 and ABZR86_RS20240 overlap by 4 nucleotides ABZR86_RS20240 and ABZR86_RS20245 are separated by 15 nucleotides
ABZR86_RS20225: ABZR86_RS20225 - flagellar biosynthetic protein FliO, at 537,031 to 537,402
_RS20225
ABZR86_RS20230: ABZR86_RS20230 - flagellar type III secretion system pore protein FliP, at 537,402 to 538,142
_RS20230
ABZR86_RS20235: ABZR86_RS20235 - flagellar biosynthesis protein FliQ, at 538,240 to 538,509
_RS20235
ABZR86_RS20240: ABZR86_RS20240 - flagellar biosynthetic protein FliR, at 538,506 to 539,276
_RS20240
ABZR86_RS20245: ABZR86_RS20245 - flagellar biosynthesis protein FlhB, at 539,292 to 540,425
_RS20245
Position (kb)
538
539 Strain fitness (log2 ratio)
-1
0
1 at 537.408 kb on + strand at 537.750 kb on + strand, within ABZR86_RS20230 at 537.750 kb on + strand, within ABZR86_RS20230 at 537.751 kb on - strand, within ABZR86_RS20230 at 537.752 kb on + strand, within ABZR86_RS20230 at 537.753 kb on - strand, within ABZR86_RS20230 at 537.753 kb on - strand, within ABZR86_RS20230 at 537.753 kb on - strand, within ABZR86_RS20230 at 538.087 kb on + strand at 538.171 kb on - strand at 538.171 kb on - strand at 538.209 kb on + strand at 538.283 kb on - strand, within ABZR86_RS20235 at 538.511 kb on + strand at 538.511 kb on + strand at 538.512 kb on - strand at 538.512 kb on - strand at 538.887 kb on + strand, within ABZR86_RS20240 at 538.888 kb on - strand, within ABZR86_RS20240 at 539.472 kb on + strand, within ABZR86_RS20245 at 539.472 kb on + strand, within ABZR86_RS20245 at 539.473 kb on - strand, within ABZR86_RS20245
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 537,408 + +0.6 537,750 + ABZR86_RS20230 0.47 +1.2 537,750 + ABZR86_RS20230 0.47 +0.1 537,751 - ABZR86_RS20230 0.47 +0.4 537,752 + ABZR86_RS20230 0.47 -1.5 537,753 - ABZR86_RS20230 0.47 +0.5 537,753 - ABZR86_RS20230 0.47 -0.4 537,753 - ABZR86_RS20230 0.47 -0.3 538,087 + -0.9 538,171 - +0.8 538,171 - -0.1 538,209 + -0.0 538,283 - ABZR86_RS20235 0.16 +0.2 538,511 + -0.1 538,511 + -0.9 538,512 - +1.3 538,512 - +0.8 538,887 + ABZR86_RS20240 0.49 +0.3 538,888 - ABZR86_RS20240 0.50 -1.6 539,472 + ABZR86_RS20245 0.16 +0.2 539,472 + ABZR86_RS20245 0.16 -0.8 539,473 - ABZR86_RS20245 0.16 +1.0
Or see this region's nucleotide sequence