Experiment: 5-Hydroxymethylfurfural 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1903 and Shewana3_1904 are separated by 73 nucleotides Shewana3_1904 and Shewana3_1905 are separated by 360 nucleotides Shewana3_1905 and Shewana3_1906 are separated by 28 nucleotides Shewana3_1906 and Shewana3_1907 are separated by 82 nucleotides
Shewana3_1903: Shewana3_1903 - restriction endonuclease (RefSeq), at 2,259,683 to 2,261,605
_1903
Shewana3_1904: Shewana3_1904 - GCN5-related N-acetyltransferase (RefSeq), at 2,261,679 to 2,262,101
_1904
Shewana3_1905: Shewana3_1905 - hypothetical protein (RefSeq), at 2,262,462 to 2,262,698
_1905
Shewana3_1906: Shewana3_1906 - 3-deoxy-manno-octulosonate cytidylyltransferase (RefSeq), at 2,262,727 to 2,263,464
_1906
Shewana3_1907: Shewana3_1907 - iron-containing alcohol dehydrogenase (RefSeq), at 2,263,547 to 2,264,617
_1907
Position (kb)
2262
2263 Strain fitness (log2 ratio)
-1
0
1
2 at 2261.635 kb on + strand at 2261.636 kb on + strand at 2261.744 kb on + strand, within Shewana3_1904 at 2262.050 kb on - strand, within Shewana3_1904 at 2262.098 kb on - strand at 2262.161 kb on - strand at 2262.245 kb on - strand at 2262.385 kb on + strand at 2262.646 kb on - strand, within Shewana3_1905 at 2262.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM remove 2,261,635 + +0.1 2,261,636 + +0.9 2,261,744 + Shewana3_1904 0.15 -1.7 2,262,050 - Shewana3_1904 0.88 -0.7 2,262,098 - +1.9 2,262,161 - -0.4 2,262,245 - +0.9 2,262,385 + +0.9 2,262,646 - Shewana3_1905 0.78 -0.2 2,262,745 - +1.9
Or see this region's nucleotide sequence