Experiment: 5-Hydroxymethylfurfural 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1096 and Shewana3_1097 are separated by 7 nucleotides Shewana3_1097 and Shewana3_1098 are separated by 67 nucleotides Shewana3_1098 and ribH are separated by 73 nucleotides ribH and nusB are separated by 14 nucleotides
Shewana3_1096: Shewana3_1096 - 5-amino-6-(5-phosphoribosylamino)uracil reductase / diaminohydroxyphosphoribosylaminopyrimidine deaminase (RefSeq), at 1,290,407 to 1,291,552
_1096
Shewana3_1097: Shewana3_1097 - riboflavin synthase subunit alpha (RefSeq), at 1,291,560 to 1,292,216
_1097
Shewana3_1098: Shewana3_1098 - GTP cyclohydrolase II / 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 1,292,284 to 1,293,387
_1098
Shewana3_1099: ribH - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq), at 1,293,461 to 1,294,003
ribH
Shewana3_1100: nusB - transcription antitermination protein NusB (RefSeq), at 1,294,018 to 1,294,422
nusB
Position (kb)
1292
1293
1294 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1291.700 kb on + strand, within Shewana3_1097 at 1291.784 kb on + strand, within Shewana3_1097 at 1291.854 kb on - strand, within Shewana3_1097 at 1291.882 kb on + strand, within Shewana3_1097 at 1291.887 kb on + strand, within Shewana3_1097 at 1291.890 kb on - strand, within Shewana3_1097 at 1291.995 kb on - strand, within Shewana3_1097 at 1292.042 kb on + strand, within Shewana3_1097 at 1292.297 kb on + strand at 1292.300 kb on - strand at 1292.305 kb on - strand at 1292.305 kb on - strand at 1292.438 kb on + strand, within Shewana3_1098 at 1292.563 kb on + strand, within Shewana3_1098 at 1292.626 kb on + strand, within Shewana3_1098 at 1292.682 kb on + strand, within Shewana3_1098 at 1292.692 kb on + strand, within Shewana3_1098 at 1292.700 kb on - strand, within Shewana3_1098 at 1292.700 kb on - strand, within Shewana3_1098 at 1292.707 kb on + strand, within Shewana3_1098 at 1292.863 kb on - strand, within Shewana3_1098 at 1293.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM remove 1,291,700 + Shewana3_1097 0.21 +0.1 1,291,784 + Shewana3_1097 0.34 +0.7 1,291,854 - Shewana3_1097 0.45 +0.3 1,291,882 + Shewana3_1097 0.49 +0.7 1,291,887 + Shewana3_1097 0.50 +0.3 1,291,890 - Shewana3_1097 0.50 -1.4 1,291,995 - Shewana3_1097 0.66 +0.6 1,292,042 + Shewana3_1097 0.73 +0.3 1,292,297 + -0.4 1,292,300 - +1.6 1,292,305 - +1.6 1,292,305 - -0.4 1,292,438 + Shewana3_1098 0.14 +3.7 1,292,563 + Shewana3_1098 0.25 +0.2 1,292,626 + Shewana3_1098 0.31 -0.1 1,292,682 + Shewana3_1098 0.36 +0.2 1,292,692 + Shewana3_1098 0.37 +0.3 1,292,700 - Shewana3_1098 0.38 +3.2 1,292,700 - Shewana3_1098 0.38 +2.0 1,292,707 + Shewana3_1098 0.38 +0.6 1,292,863 - Shewana3_1098 0.52 -0.4 1,293,298 - +0.8
Or see this region's nucleotide sequence