Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1303 and Shew_1304 are separated by 14 nucleotides Shew_1304 and Shew_1305 are separated by 572 nucleotides
Shew_1303: Shew_1303 - cytochrome d ubiquinol oxidase, subunit II (RefSeq), at 1,511,059 to 1,512,198
_1303
Shew_1304: Shew_1304 - cyd operon protein YbgT (RefSeq), at 1,512,213 to 1,512,329
_1304
Shew_1305: Shew_1305 - HNH endonuclease (RefSeq), at 1,512,902 to 1,513,342
_1305
Position (kb)
1512
1513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1511.714 kb on + strand, within Shew_1303 at 1511.778 kb on + strand, within Shew_1303 at 1512.203 kb on + strand at 1512.307 kb on - strand, within Shew_1304 at 1512.404 kb on + strand at 1512.412 kb on - strand at 1512.874 kb on - strand at 1512.934 kb on + strand at 1512.972 kb on + strand, within Shew_1305 at 1513.018 kb on + strand, within Shew_1305 at 1513.026 kb on - strand, within Shew_1305 at 1513.174 kb on - strand, within Shew_1305 at 1513.202 kb on + strand, within Shew_1305 at 1513.206 kb on + strand, within Shew_1305 at 1513.206 kb on + strand, within Shew_1305 at 1513.214 kb on - strand, within Shew_1305 at 1513.214 kb on - strand, within Shew_1305 at 1513.312 kb on + strand at 1513.312 kb on + strand at 1513.312 kb on + strand at 1513.320 kb on - strand at 1513.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,511,714 + Shew_1303 0.57 +0.2 1,511,778 + Shew_1303 0.63 -3.0 1,512,203 + +0.4 1,512,307 - Shew_1304 0.80 +0.5 1,512,404 + -0.8 1,512,412 - +0.3 1,512,874 - -0.2 1,512,934 + -0.3 1,512,972 + Shew_1305 0.16 +0.2 1,513,018 + Shew_1305 0.26 +0.0 1,513,026 - Shew_1305 0.28 +1.5 1,513,174 - Shew_1305 0.62 -0.0 1,513,202 + Shew_1305 0.68 +0.5 1,513,206 + Shew_1305 0.69 -0.9 1,513,206 + Shew_1305 0.69 +0.3 1,513,214 - Shew_1305 0.71 +0.3 1,513,214 - Shew_1305 0.71 +0.8 1,513,312 + +0.4 1,513,312 + +2.1 1,513,312 + -0.2 1,513,320 - -0.7 1,513,321 - +0.8
Or see this region's nucleotide sequence