Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0542 and Shew_0543 are separated by 4 nucleotides Shew_0543 and tolC are separated by 67 nucleotides tolC and Shew_0545 are separated by 263 nucleotides Shew_0545 and Shew_0546 overlap by 4 nucleotides
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Shew_0545: Shew_0545 - ADP-ribose diphosphatase (RefSeq), at 616,103 to 616,711
_0545
Shew_0546: Shew_0546 - hypothetical protein (RefSeq), at 616,708 to 617,154
_0546
Position (kb) 614
615
616 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand at 613.702 kb on - strand at 614.025 kb on + strand, within Shew_0543 at 614.249 kb on - strand, within Shew_0543 at 614.414 kb on - strand at 614.637 kb on - strand, within tolC at 614.676 kb on + strand, within tolC at 615.022 kb on + strand, within tolC at 615.922 kb on + strand at 615.992 kb on + strand at 616.296 kb on + strand, within Shew_0545 at 616.304 kb on - strand, within Shew_0545 at 616.347 kb on + strand, within Shew_0545 at 616.414 kb on - strand, within Shew_0545 at 616.439 kb on - strand, within Shew_0545 at 616.700 kb on - strand at 616.781 kb on - strand, within Shew_0546 at 616.795 kb on + strand, within Shew_0546
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 613,564 - Shew_0542 0.90 +0.5 613,581 - -0.3 613,702 - +0.7 614,025 + Shew_0543 0.47 +0.4 614,249 - Shew_0543 0.77 +0.4 614,414 - +0.1 614,637 - tolC Shew_0544 0.11 -5.6 614,676 + tolC Shew_0544 0.14 -4.5 615,022 + tolC Shew_0544 0.39 -2.0 615,922 + -1.1 615,992 + -0.1 616,296 + Shew_0545 0.32 +0.2 616,304 - Shew_0545 0.33 -0.4 616,347 + Shew_0545 0.40 +0.1 616,414 - Shew_0545 0.51 +0.0 616,439 - Shew_0545 0.55 -0.1 616,700 - +0.4 616,781 - Shew_0546 0.16 +0.2 616,795 + Shew_0546 0.19 -0.6
Or see this region's nucleotide sequence