Experiment: Dimethyl Sulfoxide 6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1990 and Ac3H11_1991 are separated by 89 nucleotides Ac3H11_1991 and Ac3H11_1992 are separated by 119 nucleotides Ac3H11_1992 and Ac3H11_1993 are separated by 113 nucleotides Ac3H11_1993 and Ac3H11_1994 overlap by 4 nucleotides
Ac3H11_1990 - Transcriptional regulator, LysR family, at 207,082 to 207,957
_1990
Ac3H11_1991 - hypothetical protein, at 208,047 to 208,244
_1991
Ac3H11_1992 - Glutathione S-transferase (EC 2.5.1.18), at 208,364 to 209,068
_1992
Ac3H11_1993 - Diaminopimelate epimerase (EC 5.1.1.7), at 209,182 to 210,042
_1993
Ac3H11_1994 - Protein of unknown function DUF484, at 210,039 to 210,731
_1994
Position (kb)
208
209
210 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 207.424 kb on + strand, within Ac3H11_1990 at 207.425 kb on - strand, within Ac3H11_1990 at 207.463 kb on + strand, within Ac3H11_1990 at 207.463 kb on + strand, within Ac3H11_1990 at 207.464 kb on - strand, within Ac3H11_1990 at 207.464 kb on - strand, within Ac3H11_1990 at 207.464 kb on - strand, within Ac3H11_1990 at 207.464 kb on - strand, within Ac3H11_1990 at 207.583 kb on + strand, within Ac3H11_1990 at 207.584 kb on - strand, within Ac3H11_1990 at 207.883 kb on + strand at 207.883 kb on + strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.884 kb on - strand at 207.886 kb on + strand at 208.279 kb on + strand at 208.279 kb on + strand at 208.280 kb on - strand at 208.280 kb on - strand at 208.280 kb on - strand at 208.280 kb on - strand at 208.280 kb on - strand at 208.280 kb on - strand at 208.479 kb on + strand, within Ac3H11_1992 at 208.479 kb on + strand, within Ac3H11_1992 at 208.479 kb on + strand, within Ac3H11_1992 at 208.533 kb on + strand, within Ac3H11_1992 at 208.533 kb on + strand, within Ac3H11_1992 at 208.533 kb on + strand, within Ac3H11_1992 at 208.533 kb on + strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.534 kb on - strand, within Ac3H11_1992 at 208.581 kb on - strand, within Ac3H11_1992 at 208.611 kb on + strand, within Ac3H11_1992 at 208.612 kb on - strand, within Ac3H11_1992 at 208.612 kb on - strand, within Ac3H11_1992 at 208.614 kb on - strand, within Ac3H11_1992 at 208.782 kb on + strand, within Ac3H11_1992 at 208.783 kb on - strand, within Ac3H11_1992 at 208.783 kb on - strand, within Ac3H11_1992 at 208.783 kb on - strand, within Ac3H11_1992 at 208.935 kb on + strand, within Ac3H11_1992 at 208.935 kb on + strand, within Ac3H11_1992 at 208.935 kb on + strand, within Ac3H11_1992 at 208.935 kb on + strand, within Ac3H11_1992 at 208.936 kb on - strand, within Ac3H11_1992 at 208.936 kb on - strand, within Ac3H11_1992 at 208.936 kb on - strand, within Ac3H11_1992 at 208.999 kb on - strand at 209.113 kb on + strand at 209.114 kb on - strand at 209.114 kb on - strand at 209.149 kb on + strand at 209.149 kb on + strand at 209.149 kb on + strand at 210.038 kb on + strand at 210.038 kb on + strand at 210.038 kb on + strand at 210.038 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6 vol% remove 207,424 + Ac3H11_1990 0.39 -0.9 207,425 - Ac3H11_1990 0.39 -1.0 207,463 + Ac3H11_1990 0.43 -1.6 207,463 + Ac3H11_1990 0.43 +0.1 207,464 - Ac3H11_1990 0.44 +0.5 207,464 - Ac3H11_1990 0.44 -0.7 207,464 - Ac3H11_1990 0.44 -1.4 207,464 - Ac3H11_1990 0.44 -1.2 207,583 + Ac3H11_1990 0.57 -1.0 207,584 - Ac3H11_1990 0.57 +0.0 207,883 + +0.7 207,883 + -0.7 207,884 - +0.4 207,884 - -0.2 207,884 - -1.8 207,884 - +0.3 207,884 - +0.5 207,884 - -1.2 207,886 + +2.6 208,279 + +0.5 208,279 + -0.3 208,280 - -0.2 208,280 - -1.1 208,280 - -1.6 208,280 - -0.1 208,280 - -0.2 208,280 - +0.3 208,479 + Ac3H11_1992 0.16 -0.7 208,479 + Ac3H11_1992 0.16 +0.5 208,479 + Ac3H11_1992 0.16 -0.1 208,533 + Ac3H11_1992 0.24 -1.6 208,533 + Ac3H11_1992 0.24 +0.1 208,533 + Ac3H11_1992 0.24 +1.1 208,533 + Ac3H11_1992 0.24 -0.5 208,534 - Ac3H11_1992 0.24 +0.2 208,534 - Ac3H11_1992 0.24 -0.1 208,534 - Ac3H11_1992 0.24 +1.6 208,534 - Ac3H11_1992 0.24 -0.2 208,534 - Ac3H11_1992 0.24 +0.8 208,534 - Ac3H11_1992 0.24 +0.0 208,581 - Ac3H11_1992 0.31 +0.4 208,611 + Ac3H11_1992 0.35 +0.1 208,612 - Ac3H11_1992 0.35 +0.3 208,612 - Ac3H11_1992 0.35 -0.1 208,614 - Ac3H11_1992 0.35 +0.1 208,782 + Ac3H11_1992 0.59 +1.2 208,783 - Ac3H11_1992 0.59 -1.8 208,783 - Ac3H11_1992 0.59 +1.1 208,783 - Ac3H11_1992 0.59 +1.1 208,935 + Ac3H11_1992 0.81 -0.8 208,935 + Ac3H11_1992 0.81 -0.5 208,935 + Ac3H11_1992 0.81 -1.5 208,935 + Ac3H11_1992 0.81 -1.0 208,936 - Ac3H11_1992 0.81 -0.2 208,936 - Ac3H11_1992 0.81 +0.5 208,936 - Ac3H11_1992 0.81 -0.6 208,999 - -1.0 209,113 + -0.4 209,114 - -0.1 209,114 - -0.0 209,149 + -0.5 209,149 + +0.1 209,149 + +0.5 210,038 + -0.1 210,038 + +1.1 210,038 + -0.3 210,038 + +0.8
Or see this region's nucleotide sequence