Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS06470 and ABZR86_RS06475 are separated by 25 nucleotides ABZR86_RS06475 and ABZR86_RS06480 are separated by 596 nucleotides ABZR86_RS06480 and ABZR86_RS06485 overlap by 4 nucleotides ABZR86_RS06485 and ABZR86_RS06490 overlap by 4 nucleotides
ABZR86_RS06470: ABZR86_RS06470 - cysteine dioxygenase family protein, at 1,435,626 to 1,436,210
_RS06470
ABZR86_RS06475: ABZR86_RS06475 - tRNA-Leu, at 1,436,236 to 1,436,321
_RS06475
ABZR86_RS06480: ABZR86_RS06480 - SIR2 family protein, at 1,436,918 to 1,438,621
_RS06480
ABZR86_RS06485: ABZR86_RS06485 - hypothetical protein, at 1,438,618 to 1,439,205
_RS06485
ABZR86_RS06490: ABZR86_RS06490 - AAA family ATPase, at 1,439,202 to 1,441,652
_RS06490
Position (kb)
1436
1437
1438
1439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1435.942 kb on + strand, within ABZR86_RS06470 at 1435.942 kb on + strand, within ABZR86_RS06470 at 1435.950 kb on + strand, within ABZR86_RS06470 at 1435.951 kb on - strand, within ABZR86_RS06470 at 1436.052 kb on + strand, within ABZR86_RS06470 at 1436.052 kb on + strand, within ABZR86_RS06470 at 1436.053 kb on - strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.106 kb on + strand, within ABZR86_RS06470 at 1436.107 kb on - strand, within ABZR86_RS06470 at 1436.107 kb on - strand, within ABZR86_RS06470 at 1436.107 kb on - strand, within ABZR86_RS06470 at 1436.107 kb on - strand, within ABZR86_RS06470 at 1436.166 kb on + strand at 1436.166 kb on + strand at 1436.167 kb on - strand at 1436.167 kb on - strand at 1436.327 kb on + strand at 1436.345 kb on + strand at 1436.345 kb on + strand at 1436.345 kb on + strand at 1436.346 kb on - strand at 1436.379 kb on + strand at 1436.380 kb on - strand at 1436.380 kb on - strand at 1436.394 kb on - strand at 1436.394 kb on - strand at 1436.394 kb on - strand at 1436.399 kb on + strand at 1436.399 kb on + strand at 1436.400 kb on - strand at 1436.410 kb on + strand at 1436.553 kb on + strand at 1436.554 kb on - strand at 1436.554 kb on - strand at 1436.641 kb on + strand at 1436.641 kb on + strand at 1436.641 kb on + strand at 1436.641 kb on + strand at 1436.641 kb on + strand at 1436.641 kb on + strand at 1436.642 kb on - strand at 1436.642 kb on - strand at 1436.642 kb on - strand at 1436.659 kb on + strand at 1436.659 kb on + strand at 1436.660 kb on - strand at 1436.726 kb on + strand at 1436.727 kb on - strand at 1436.814 kb on + strand at 1436.814 kb on + strand at 1436.822 kb on + strand at 1436.826 kb on + strand at 1436.826 kb on + strand at 1436.826 kb on + strand at 1436.826 kb on + strand at 1436.827 kb on - strand at 1436.836 kb on + strand at 1436.880 kb on + strand at 1436.880 kb on + strand at 1436.880 kb on + strand at 1436.880 kb on + strand at 1436.881 kb on - strand at 1436.883 kb on + strand at 1436.884 kb on - strand at 1436.894 kb on + strand at 1436.894 kb on + strand at 1436.895 kb on - strand at 1436.898 kb on + strand at 1436.923 kb on + strand at 1436.923 kb on + strand at 1436.924 kb on - strand at 1436.924 kb on - strand at 1436.924 kb on - strand at 1436.936 kb on - strand at 1436.936 kb on - strand at 1436.944 kb on + strand at 1437.011 kb on + strand at 1437.011 kb on + strand at 1437.011 kb on + strand at 1437.012 kb on - strand at 1437.124 kb on + strand, within ABZR86_RS06480 at 1437.124 kb on + strand, within ABZR86_RS06480 at 1437.124 kb on + strand, within ABZR86_RS06480 at 1437.125 kb on - strand, within ABZR86_RS06480 at 1437.125 kb on - strand, within ABZR86_RS06480 at 1437.125 kb on - strand, within ABZR86_RS06480 at 1437.125 kb on - strand, within ABZR86_RS06480 at 1437.128 kb on + strand, within ABZR86_RS06480 at 1437.234 kb on + strand, within ABZR86_RS06480 at 1437.235 kb on - strand, within ABZR86_RS06480 at 1437.283 kb on + strand, within ABZR86_RS06480 at 1437.283 kb on + strand, within ABZR86_RS06480 at 1437.284 kb on - strand, within ABZR86_RS06480 at 1437.305 kb on + strand, within ABZR86_RS06480 at 1437.305 kb on + strand, within ABZR86_RS06480 at 1437.305 kb on + strand, within ABZR86_RS06480 at 1437.372 kb on + strand, within ABZR86_RS06480 at 1437.373 kb on - strand, within ABZR86_RS06480 at 1437.373 kb on - strand, within ABZR86_RS06480 at 1437.406 kb on - strand, within ABZR86_RS06480 at 1437.419 kb on - strand, within ABZR86_RS06480 at 1437.419 kb on - strand, within ABZR86_RS06480 at 1437.482 kb on + strand, within ABZR86_RS06480 at 1437.483 kb on - strand, within ABZR86_RS06480 at 1437.483 kb on - strand, within ABZR86_RS06480 at 1437.495 kb on + strand, within ABZR86_RS06480 at 1437.495 kb on + strand, within ABZR86_RS06480 at 1437.539 kb on + strand, within ABZR86_RS06480 at 1437.539 kb on + strand, within ABZR86_RS06480 at 1437.540 kb on - strand, within ABZR86_RS06480 at 1437.611 kb on - strand, within ABZR86_RS06480 at 1437.634 kb on + strand, within ABZR86_RS06480 at 1437.634 kb on + strand, within ABZR86_RS06480 at 1437.635 kb on - strand, within ABZR86_RS06480 at 1437.670 kb on + strand, within ABZR86_RS06480 at 1437.671 kb on - strand, within ABZR86_RS06480 at 1437.671 kb on - strand, within ABZR86_RS06480 at 1437.753 kb on + strand, within ABZR86_RS06480 at 1437.854 kb on + strand, within ABZR86_RS06480 at 1437.855 kb on - strand, within ABZR86_RS06480 at 1437.870 kb on - strand, within ABZR86_RS06480 at 1437.917 kb on + strand, within ABZR86_RS06480 at 1437.933 kb on + strand, within ABZR86_RS06480 at 1438.042 kb on + strand, within ABZR86_RS06480 at 1438.043 kb on - strand, within ABZR86_RS06480 at 1438.043 kb on - strand, within ABZR86_RS06480 at 1438.043 kb on - strand, within ABZR86_RS06480 at 1438.098 kb on + strand, within ABZR86_RS06480 at 1438.098 kb on + strand, within ABZR86_RS06480 at 1438.098 kb on + strand, within ABZR86_RS06480 at 1438.099 kb on - strand, within ABZR86_RS06480 at 1438.099 kb on - strand, within ABZR86_RS06480 at 1438.099 kb on - strand, within ABZR86_RS06480 at 1438.099 kb on - strand, within ABZR86_RS06480 at 1438.099 kb on - strand, within ABZR86_RS06480 at 1438.175 kb on + strand, within ABZR86_RS06480 at 1438.175 kb on + strand, within ABZR86_RS06480 at 1438.176 kb on - strand, within ABZR86_RS06480 at 1438.176 kb on - strand, within ABZR86_RS06480 at 1438.176 kb on - strand, within ABZR86_RS06480 at 1438.198 kb on + strand, within ABZR86_RS06480 at 1438.198 kb on + strand, within ABZR86_RS06480 at 1438.198 kb on + strand, within ABZR86_RS06480 at 1438.199 kb on - strand, within ABZR86_RS06480 at 1438.199 kb on - strand, within ABZR86_RS06480 at 1438.199 kb on - strand, within ABZR86_RS06480 at 1438.207 kb on + strand, within ABZR86_RS06480 at 1438.207 kb on + strand, within ABZR86_RS06480 at 1438.208 kb on - strand, within ABZR86_RS06480 at 1438.208 kb on - strand, within ABZR86_RS06480 at 1438.378 kb on + strand, within ABZR86_RS06480 at 1438.400 kb on - strand, within ABZR86_RS06480 at 1438.538 kb on + strand at 1438.538 kb on + strand at 1438.538 kb on + strand at 1438.538 kb on + strand at 1438.539 kb on - strand at 1438.539 kb on - strand at 1438.539 kb on - strand at 1438.539 kb on - strand at 1438.539 kb on - strand at 1438.587 kb on + strand at 1438.617 kb on + strand at 1438.637 kb on + strand at 1438.638 kb on - strand at 1438.638 kb on - strand at 1438.638 kb on - strand at 1438.644 kb on + strand at 1438.645 kb on - strand at 1438.645 kb on - strand at 1438.673 kb on + strand at 1438.694 kb on + strand, within ABZR86_RS06485 at 1438.694 kb on + strand, within ABZR86_RS06485 at 1438.695 kb on - strand, within ABZR86_RS06485 at 1438.703 kb on + strand, within ABZR86_RS06485 at 1438.704 kb on - strand, within ABZR86_RS06485 at 1438.720 kb on + strand, within ABZR86_RS06485 at 1438.721 kb on - strand, within ABZR86_RS06485 at 1438.773 kb on + strand, within ABZR86_RS06485 at 1438.783 kb on - strand, within ABZR86_RS06485 at 1438.821 kb on + strand, within ABZR86_RS06485 at 1438.931 kb on + strand, within ABZR86_RS06485 at 1438.932 kb on - strand, within ABZR86_RS06485 at 1438.932 kb on - strand, within ABZR86_RS06485 at 1438.962 kb on + strand, within ABZR86_RS06485 at 1438.962 kb on + strand, within ABZR86_RS06485 at 1438.962 kb on + strand, within ABZR86_RS06485 at 1438.963 kb on - strand, within ABZR86_RS06485 at 1438.963 kb on - strand, within ABZR86_RS06485 at 1438.963 kb on - strand, within ABZR86_RS06485 at 1438.997 kb on + strand, within ABZR86_RS06485 at 1438.997 kb on + strand, within ABZR86_RS06485 at 1438.998 kb on - strand, within ABZR86_RS06485 at 1439.071 kb on + strand, within ABZR86_RS06485 at 1439.071 kb on + strand, within ABZR86_RS06485 at 1439.144 kb on + strand, within ABZR86_RS06485 at 1439.162 kb on + strand at 1439.162 kb on + strand at 1439.163 kb on - strand at 1439.394 kb on + strand at 1439.395 kb on - strand at 1439.395 kb on - strand at 1439.395 kb on - strand at 1439.404 kb on - strand at 1439.408 kb on + strand at 1439.408 kb on + strand at 1439.408 kb on + strand at 1439.409 kb on - strand at 1439.409 kb on - strand at 1439.409 kb on - strand at 1439.459 kb on + strand, within ABZR86_RS06490 at 1439.528 kb on - strand, within ABZR86_RS06490 at 1439.568 kb on + strand, within ABZR86_RS06490 at 1439.568 kb on + strand, within ABZR86_RS06490 at 1439.568 kb on + strand, within ABZR86_RS06490 at 1439.569 kb on - strand, within ABZR86_RS06490 at 1439.569 kb on - strand, within ABZR86_RS06490 at 1439.607 kb on + strand, within ABZR86_RS06490 at 1439.608 kb on - strand, within ABZR86_RS06490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,435,942 + ABZR86_RS06470 0.54 +0.8 1,435,942 + ABZR86_RS06470 0.54 +1.3 1,435,950 + ABZR86_RS06470 0.55 -0.8 1,435,951 - ABZR86_RS06470 0.56 +1.4 1,436,052 + ABZR86_RS06470 0.73 -0.2 1,436,052 + ABZR86_RS06470 0.73 +1.2 1,436,053 - ABZR86_RS06470 0.73 +1.3 1,436,106 + ABZR86_RS06470 0.82 -0.3 1,436,106 + ABZR86_RS06470 0.82 +0.1 1,436,106 + ABZR86_RS06470 0.82 -0.7 1,436,106 + ABZR86_RS06470 0.82 +0.7 1,436,106 + ABZR86_RS06470 0.82 +0.2 1,436,106 + ABZR86_RS06470 0.82 -0.4 1,436,107 - ABZR86_RS06470 0.82 +0.3 1,436,107 - ABZR86_RS06470 0.82 +0.6 1,436,107 - ABZR86_RS06470 0.82 +0.5 1,436,107 - ABZR86_RS06470 0.82 +0.1 1,436,166 + +0.5 1,436,166 + -0.7 1,436,167 - +0.4 1,436,167 - +0.2 1,436,327 + -1.0 1,436,345 + -0.2 1,436,345 + -0.2 1,436,345 + +0.1 1,436,346 - -0.7 1,436,379 + -1.6 1,436,380 - +0.3 1,436,380 - +0.5 1,436,394 - -0.6 1,436,394 - +0.1 1,436,394 - +0.2 1,436,399 + -0.2 1,436,399 + +0.1 1,436,400 - -0.5 1,436,410 + -0.7 1,436,553 + +0.3 1,436,554 - +1.2 1,436,554 - -0.1 1,436,641 + -0.4 1,436,641 + +0.8 1,436,641 + +0.3 1,436,641 + +0.2 1,436,641 + +0.3 1,436,641 + +0.7 1,436,642 - -0.3 1,436,642 - -0.4 1,436,642 - -0.5 1,436,659 + +0.0 1,436,659 + -0.4 1,436,660 - +1.6 1,436,726 + -0.9 1,436,727 - -0.4 1,436,814 + +1.5 1,436,814 + +0.1 1,436,822 + -0.2 1,436,826 + -0.3 1,436,826 + -0.5 1,436,826 + +0.2 1,436,826 + +0.2 1,436,827 - +0.6 1,436,836 + +0.6 1,436,880 + +0.4 1,436,880 + +0.6 1,436,880 + -0.8 1,436,880 + +0.4 1,436,881 - -0.0 1,436,883 + +0.7 1,436,884 - -0.1 1,436,894 + -0.8 1,436,894 + -0.1 1,436,895 - +0.1 1,436,898 + -1.1 1,436,923 + +0.4 1,436,923 + -0.6 1,436,924 - +0.1 1,436,924 - +0.3 1,436,924 - +0.1 1,436,936 - -0.3 1,436,936 - +0.3 1,436,944 + +0.1 1,437,011 + +0.9 1,437,011 + -1.3 1,437,011 + +0.1 1,437,012 - -2.8 1,437,124 + ABZR86_RS06480 0.12 -0.1 1,437,124 + ABZR86_RS06480 0.12 +0.1 1,437,124 + ABZR86_RS06480 0.12 +0.9 1,437,125 - ABZR86_RS06480 0.12 -0.2 1,437,125 - ABZR86_RS06480 0.12 -0.3 1,437,125 - ABZR86_RS06480 0.12 +1.6 1,437,125 - ABZR86_RS06480 0.12 -0.4 1,437,128 + ABZR86_RS06480 0.12 +0.2 1,437,234 + ABZR86_RS06480 0.19 -1.1 1,437,235 - ABZR86_RS06480 0.19 +0.7 1,437,283 + ABZR86_RS06480 0.21 +0.8 1,437,283 + ABZR86_RS06480 0.21 +0.2 1,437,284 - ABZR86_RS06480 0.21 +0.2 1,437,305 + ABZR86_RS06480 0.23 +1.2 1,437,305 + ABZR86_RS06480 0.23 -0.1 1,437,305 + ABZR86_RS06480 0.23 +0.4 1,437,372 + ABZR86_RS06480 0.27 -0.2 1,437,373 - ABZR86_RS06480 0.27 +0.3 1,437,373 - ABZR86_RS06480 0.27 -0.1 1,437,406 - ABZR86_RS06480 0.29 -0.5 1,437,419 - ABZR86_RS06480 0.29 -0.7 1,437,419 - ABZR86_RS06480 0.29 +0.1 1,437,482 + ABZR86_RS06480 0.33 +0.8 1,437,483 - ABZR86_RS06480 0.33 -0.3 1,437,483 - ABZR86_RS06480 0.33 -0.6 1,437,495 + ABZR86_RS06480 0.34 -0.2 1,437,495 + ABZR86_RS06480 0.34 +0.6 1,437,539 + ABZR86_RS06480 0.36 -1.4 1,437,539 + ABZR86_RS06480 0.36 -0.1 1,437,540 - ABZR86_RS06480 0.37 -0.5 1,437,611 - ABZR86_RS06480 0.41 -0.2 1,437,634 + ABZR86_RS06480 0.42 +1.7 1,437,634 + ABZR86_RS06480 0.42 -0.1 1,437,635 - ABZR86_RS06480 0.42 -0.7 1,437,670 + ABZR86_RS06480 0.44 +0.2 1,437,671 - ABZR86_RS06480 0.44 -1.1 1,437,671 - ABZR86_RS06480 0.44 +0.2 1,437,753 + ABZR86_RS06480 0.49 -0.5 1,437,854 + ABZR86_RS06480 0.55 +0.6 1,437,855 - ABZR86_RS06480 0.55 +0.1 1,437,870 - ABZR86_RS06480 0.56 -1.1 1,437,917 + ABZR86_RS06480 0.59 +0.4 1,437,933 + ABZR86_RS06480 0.60 +0.4 1,438,042 + ABZR86_RS06480 0.66 +0.5 1,438,043 - ABZR86_RS06480 0.66 -0.1 1,438,043 - ABZR86_RS06480 0.66 +0.4 1,438,043 - ABZR86_RS06480 0.66 +0.7 1,438,098 + ABZR86_RS06480 0.69 -0.1 1,438,098 + ABZR86_RS06480 0.69 +0.3 1,438,098 + ABZR86_RS06480 0.69 -0.3 1,438,099 - ABZR86_RS06480 0.69 +0.5 1,438,099 - ABZR86_RS06480 0.69 -0.4 1,438,099 - ABZR86_RS06480 0.69 -0.5 1,438,099 - ABZR86_RS06480 0.69 +0.3 1,438,099 - ABZR86_RS06480 0.69 -0.2 1,438,175 + ABZR86_RS06480 0.74 -1.4 1,438,175 + ABZR86_RS06480 0.74 +0.0 1,438,176 - ABZR86_RS06480 0.74 +0.7 1,438,176 - ABZR86_RS06480 0.74 +0.3 1,438,176 - ABZR86_RS06480 0.74 -0.3 1,438,198 + ABZR86_RS06480 0.75 -0.1 1,438,198 + ABZR86_RS06480 0.75 -0.3 1,438,198 + ABZR86_RS06480 0.75 -0.4 1,438,199 - ABZR86_RS06480 0.75 -0.4 1,438,199 - ABZR86_RS06480 0.75 -0.5 1,438,199 - ABZR86_RS06480 0.75 -1.5 1,438,207 + ABZR86_RS06480 0.76 +1.0 1,438,207 + ABZR86_RS06480 0.76 +0.8 1,438,208 - ABZR86_RS06480 0.76 -0.5 1,438,208 - ABZR86_RS06480 0.76 -0.0 1,438,378 + ABZR86_RS06480 0.86 +0.4 1,438,400 - ABZR86_RS06480 0.87 -1.0 1,438,538 + +0.7 1,438,538 + +0.1 1,438,538 + -0.2 1,438,538 + +0.4 1,438,539 - -1.4 1,438,539 - -0.5 1,438,539 - +0.5 1,438,539 - -1.0 1,438,539 - +0.2 1,438,587 + -0.1 1,438,617 + +0.2 1,438,637 + -0.4 1,438,638 - +0.5 1,438,638 - +0.2 1,438,638 - +0.1 1,438,644 + +1.0 1,438,645 - +0.1 1,438,645 - -0.8 1,438,673 + -0.1 1,438,694 + ABZR86_RS06485 0.13 -0.0 1,438,694 + ABZR86_RS06485 0.13 +0.7 1,438,695 - ABZR86_RS06485 0.13 -0.9 1,438,703 + ABZR86_RS06485 0.14 +0.7 1,438,704 - ABZR86_RS06485 0.15 +0.1 1,438,720 + ABZR86_RS06485 0.17 -0.1 1,438,721 - ABZR86_RS06485 0.18 -0.5 1,438,773 + ABZR86_RS06485 0.26 +0.5 1,438,783 - ABZR86_RS06485 0.28 -0.4 1,438,821 + ABZR86_RS06485 0.35 +0.4 1,438,931 + ABZR86_RS06485 0.53 -0.2 1,438,932 - ABZR86_RS06485 0.53 +0.9 1,438,932 - ABZR86_RS06485 0.53 +0.6 1,438,962 + ABZR86_RS06485 0.59 -0.2 1,438,962 + ABZR86_RS06485 0.59 +0.4 1,438,962 + ABZR86_RS06485 0.59 -0.5 1,438,963 - ABZR86_RS06485 0.59 -0.5 1,438,963 - ABZR86_RS06485 0.59 +0.4 1,438,963 - ABZR86_RS06485 0.59 +0.5 1,438,997 + ABZR86_RS06485 0.64 +0.7 1,438,997 + ABZR86_RS06485 0.64 +0.2 1,438,998 - ABZR86_RS06485 0.65 +0.7 1,439,071 + ABZR86_RS06485 0.77 +0.7 1,439,071 + ABZR86_RS06485 0.77 +0.5 1,439,144 + ABZR86_RS06485 0.89 +0.4 1,439,162 + +0.2 1,439,162 + +0.4 1,439,163 - -0.3 1,439,394 + +0.1 1,439,395 - -0.8 1,439,395 - -0.5 1,439,395 - -0.4 1,439,404 - -0.7 1,439,408 + -1.5 1,439,408 + +0.6 1,439,408 + -0.6 1,439,409 - +0.1 1,439,409 - -0.3 1,439,409 - -1.8 1,439,459 + ABZR86_RS06490 0.10 -0.2 1,439,528 - ABZR86_RS06490 0.13 -0.6 1,439,568 + ABZR86_RS06490 0.15 -1.3 1,439,568 + ABZR86_RS06490 0.15 +0.1 1,439,568 + ABZR86_RS06490 0.15 +0.5 1,439,569 - ABZR86_RS06490 0.15 -0.1 1,439,569 - ABZR86_RS06490 0.15 +0.3 1,439,607 + ABZR86_RS06490 0.17 +0.3 1,439,608 - ABZR86_RS06490 0.17 +0.1
Or see this region's nucleotide sequence