Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS04700 and ABZR86_RS04705 are separated by 12 nucleotides ABZR86_RS04705 and ABZR86_RS04710 overlap by 4 nucleotides ABZR86_RS04710 and ABZR86_RS04715 are separated by 40 nucleotides
ABZR86_RS04700: ABZR86_RS04700 - radical SAM protein, at 1,053,750 to 1,055,249
_RS04700
ABZR86_RS04705: ABZR86_RS04705 - class I SAM-dependent methyltransferase, at 1,055,262 to 1,055,957
_RS04705
ABZR86_RS04710: ABZR86_RS04710 - amidohydrolase family protein, at 1,055,954 to 1,057,111
_RS04710
ABZR86_RS04715: ABZR86_RS04715 - class I SAM-dependent methyltransferase, at 1,057,152 to 1,058,012
_RS04715
Position (kb)
1055
1056
1057
1058 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1054.960 kb on + strand, within ABZR86_RS04700 at 1054.961 kb on - strand, within ABZR86_RS04700 at 1055.099 kb on + strand, within ABZR86_RS04700 at 1055.099 kb on + strand, within ABZR86_RS04700 at 1055.419 kb on + strand, within ABZR86_RS04705 at 1055.420 kb on - strand, within ABZR86_RS04705 at 1055.420 kb on - strand, within ABZR86_RS04705 at 1055.420 kb on - strand, within ABZR86_RS04705 at 1055.433 kb on - strand, within ABZR86_RS04705 at 1055.446 kb on + strand, within ABZR86_RS04705 at 1055.447 kb on - strand, within ABZR86_RS04705 at 1055.581 kb on + strand, within ABZR86_RS04705 at 1055.704 kb on + strand, within ABZR86_RS04705 at 1056.301 kb on - strand, within ABZR86_RS04710 at 1056.301 kb on - strand, within ABZR86_RS04710 at 1056.717 kb on + strand, within ABZR86_RS04710 at 1056.718 kb on - strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.924 kb on + strand, within ABZR86_RS04710 at 1056.925 kb on - strand, within ABZR86_RS04710 at 1056.925 kb on - strand, within ABZR86_RS04710 at 1056.925 kb on - strand, within ABZR86_RS04710 at 1056.925 kb on - strand, within ABZR86_RS04710 at 1056.925 kb on - strand, within ABZR86_RS04710 at 1057.010 kb on + strand at 1057.010 kb on + strand at 1057.011 kb on - strand at 1057.282 kb on + strand, within ABZR86_RS04715 at 1057.283 kb on - strand, within ABZR86_RS04715 at 1057.352 kb on - strand, within ABZR86_RS04715 at 1057.370 kb on - strand, within ABZR86_RS04715 at 1057.370 kb on - strand, within ABZR86_RS04715 at 1057.372 kb on + strand, within ABZR86_RS04715 at 1057.372 kb on + strand, within ABZR86_RS04715 at 1057.372 kb on + strand, within ABZR86_RS04715 at 1057.373 kb on - strand, within ABZR86_RS04715 at 1057.386 kb on - strand, within ABZR86_RS04715 at 1057.465 kb on + strand, within ABZR86_RS04715 at 1057.600 kb on + strand, within ABZR86_RS04715 at 1057.600 kb on + strand, within ABZR86_RS04715 at 1057.600 kb on + strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.601 kb on - strand, within ABZR86_RS04715 at 1057.674 kb on - strand, within ABZR86_RS04715 at 1057.674 kb on - strand, within ABZR86_RS04715 at 1057.921 kb on + strand, within ABZR86_RS04715 at 1057.921 kb on + strand, within ABZR86_RS04715 at 1057.922 kb on - strand, within ABZR86_RS04715
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,054,960 + ABZR86_RS04700 0.81 -0.0 1,054,961 - ABZR86_RS04700 0.81 -1.8 1,055,099 + ABZR86_RS04700 0.90 -0.6 1,055,099 + ABZR86_RS04700 0.90 +0.2 1,055,419 + ABZR86_RS04705 0.23 +0.6 1,055,420 - ABZR86_RS04705 0.23 -0.6 1,055,420 - ABZR86_RS04705 0.23 -0.5 1,055,420 - ABZR86_RS04705 0.23 +0.0 1,055,433 - ABZR86_RS04705 0.25 +1.8 1,055,446 + ABZR86_RS04705 0.26 -0.2 1,055,447 - ABZR86_RS04705 0.27 +0.6 1,055,581 + ABZR86_RS04705 0.46 -1.3 1,055,704 + ABZR86_RS04705 0.64 +0.4 1,056,301 - ABZR86_RS04710 0.30 -0.2 1,056,301 - ABZR86_RS04710 0.30 -0.4 1,056,717 + ABZR86_RS04710 0.66 +0.2 1,056,718 - ABZR86_RS04710 0.66 -0.5 1,056,924 + ABZR86_RS04710 0.84 -0.1 1,056,924 + ABZR86_RS04710 0.84 -0.3 1,056,924 + ABZR86_RS04710 0.84 -0.7 1,056,924 + ABZR86_RS04710 0.84 -1.7 1,056,924 + ABZR86_RS04710 0.84 +0.5 1,056,924 + ABZR86_RS04710 0.84 +0.1 1,056,924 + ABZR86_RS04710 0.84 +0.4 1,056,924 + ABZR86_RS04710 0.84 +0.8 1,056,924 + ABZR86_RS04710 0.84 -0.0 1,056,924 + ABZR86_RS04710 0.84 +0.4 1,056,925 - ABZR86_RS04710 0.84 -0.2 1,056,925 - ABZR86_RS04710 0.84 +0.3 1,056,925 - ABZR86_RS04710 0.84 +0.0 1,056,925 - ABZR86_RS04710 0.84 +0.2 1,056,925 - ABZR86_RS04710 0.84 +0.0 1,057,010 + +0.2 1,057,010 + -0.2 1,057,011 - -0.6 1,057,282 + ABZR86_RS04715 0.15 +0.1 1,057,283 - ABZR86_RS04715 0.15 +0.2 1,057,352 - ABZR86_RS04715 0.23 -0.3 1,057,370 - ABZR86_RS04715 0.25 -0.2 1,057,370 - ABZR86_RS04715 0.25 +0.8 1,057,372 + ABZR86_RS04715 0.26 -0.7 1,057,372 + ABZR86_RS04715 0.26 +1.0 1,057,372 + ABZR86_RS04715 0.26 -1.3 1,057,373 - ABZR86_RS04715 0.26 +0.3 1,057,386 - ABZR86_RS04715 0.27 +0.1 1,057,465 + ABZR86_RS04715 0.36 +0.0 1,057,600 + ABZR86_RS04715 0.52 +0.9 1,057,600 + ABZR86_RS04715 0.52 +0.9 1,057,600 + ABZR86_RS04715 0.52 +0.3 1,057,601 - ABZR86_RS04715 0.52 +1.9 1,057,601 - ABZR86_RS04715 0.52 -0.1 1,057,601 - ABZR86_RS04715 0.52 +0.7 1,057,601 - ABZR86_RS04715 0.52 -1.1 1,057,601 - ABZR86_RS04715 0.52 +0.8 1,057,601 - ABZR86_RS04715 0.52 +1.0 1,057,674 - ABZR86_RS04715 0.61 +0.1 1,057,674 - ABZR86_RS04715 0.61 -0.0 1,057,921 + ABZR86_RS04715 0.89 +0.5 1,057,921 + ABZR86_RS04715 0.89 +1.5 1,057,922 - ABZR86_RS04715 0.89 +0.4
Or see this region's nucleotide sequence