Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00135 and ABZR86_RS00140 are separated by 29 nucleotides ABZR86_RS00140 and ABZR86_RS00145 are separated by 282 nucleotides ABZR86_RS00145 and ABZR86_RS00150 are separated by 61 nucleotides ABZR86_RS00150 and ABZR86_RS00155 are separated by 34 nucleotides
ABZR86_RS00135: ABZR86_RS00135 - DUF899 family protein, at 26,392 to 27,096
_RS00135
ABZR86_RS00140: ABZR86_RS00140 - glutathione S-transferase family protein, at 27,126 to 27,785
_RS00140
ABZR86_RS00145: ABZR86_RS00145 - MarR family transcriptional regulator, at 28,068 to 28,535
_RS00145
ABZR86_RS00150: ABZR86_RS00150 - isochorismatase family protein, at 28,597 to 29,154
_RS00150
ABZR86_RS00155: ABZR86_RS00155 - MFS transporter, at 29,189 to 30,475
_RS00155
Position (kb) 28
29 Strain fitness (log2 ratio) -2
-1
0
1 at 27.166 kb on + strand at 27.166 kb on + strand at 27.166 kb on + strand at 27.278 kb on - strand, within ABZR86_RS00140 at 27.278 kb on - strand, within ABZR86_RS00140 at 27.278 kb on - strand, within ABZR86_RS00140 at 27.370 kb on + strand, within ABZR86_RS00140 at 27.475 kb on + strand, within ABZR86_RS00140 at 27.475 kb on + strand, within ABZR86_RS00140 at 27.475 kb on + strand, within ABZR86_RS00140 at 27.475 kb on + strand, within ABZR86_RS00140 at 27.476 kb on - strand, within ABZR86_RS00140 at 27.476 kb on - strand, within ABZR86_RS00140 at 27.476 kb on - strand, within ABZR86_RS00140 at 27.476 kb on - strand, within ABZR86_RS00140 at 27.571 kb on + strand, within ABZR86_RS00140 at 27.572 kb on - strand, within ABZR86_RS00140 at 27.748 kb on + strand at 27.748 kb on + strand at 27.769 kb on + strand at 27.901 kb on + strand at 27.932 kb on - strand at 28.017 kb on + strand at 28.018 kb on - strand at 28.048 kb on - strand at 28.672 kb on - strand, within ABZR86_RS00150 at 29.193 kb on + strand at 29.193 kb on + strand at 29.219 kb on + strand at 29.220 kb on - strand at 29.220 kb on - strand at 29.225 kb on + strand at 29.226 kb on - strand at 29.226 kb on - strand at 29.516 kb on + strand, within ABZR86_RS00155 at 29.516 kb on + strand, within ABZR86_RS00155 at 29.516 kb on + strand, within ABZR86_RS00155 at 29.516 kb on + strand, within ABZR86_RS00155 at 29.516 kb on + strand, within ABZR86_RS00155 at 29.516 kb on + strand, within ABZR86_RS00155 at 29.517 kb on - strand, within ABZR86_RS00155 at 29.517 kb on - strand, within ABZR86_RS00155
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 27,166 + -1.1 27,166 + -0.2 27,166 + -0.1 27,278 - ABZR86_RS00140 0.23 +0.5 27,278 - ABZR86_RS00140 0.23 -0.4 27,278 - ABZR86_RS00140 0.23 -0.6 27,370 + ABZR86_RS00140 0.37 -0.3 27,475 + ABZR86_RS00140 0.53 +0.3 27,475 + ABZR86_RS00140 0.53 -0.3 27,475 + ABZR86_RS00140 0.53 -0.3 27,475 + ABZR86_RS00140 0.53 -1.0 27,476 - ABZR86_RS00140 0.53 +0.9 27,476 - ABZR86_RS00140 0.53 -0.2 27,476 - ABZR86_RS00140 0.53 -0.3 27,476 - ABZR86_RS00140 0.53 -0.4 27,571 + ABZR86_RS00140 0.67 -0.4 27,572 - ABZR86_RS00140 0.68 +0.9 27,748 + -2.4 27,748 + -0.7 27,769 + -0.8 27,901 + -1.0 27,932 - -0.8 28,017 + -0.0 28,018 - -1.2 28,048 - -0.7 28,672 - ABZR86_RS00150 0.13 +0.3 29,193 + -0.6 29,193 + +0.5 29,219 + +0.3 29,220 - +0.4 29,220 - -0.2 29,225 + +0.6 29,226 - -0.3 29,226 - -0.8 29,516 + ABZR86_RS00155 0.25 -0.0 29,516 + ABZR86_RS00155 0.25 +1.0 29,516 + ABZR86_RS00155 0.25 +0.0 29,516 + ABZR86_RS00155 0.25 -0.1 29,516 + ABZR86_RS00155 0.25 -0.3 29,516 + ABZR86_RS00155 0.25 +0.4 29,517 - ABZR86_RS00155 0.25 +0.2 29,517 - ABZR86_RS00155 0.25 -0.5
Or see this region's nucleotide sequence