Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00030 and ABZR86_RS00035 are separated by 307 nucleotides ABZR86_RS00035 and ABZR86_RS00040 are separated by 246 nucleotides
ABZR86_RS00030: ABZR86_RS00030 - beta-xylosidase, at 4,551 to 6,074
_RS00030
ABZR86_RS00035: ABZR86_RS00035 - monovalent cation:proton antiporter-2 (CPA2) family protein, at 6,382 to 8,079
_RS00035
ABZR86_RS00040: ABZR86_RS00040 - hypothetical protein, at 8,326 to 9,372
_RS00040
Position (kb)
6
7
8
9 Strain fitness (log2 ratio)
-1
0
1 at 5.519 kb on - strand, within ABZR86_RS00030 at 5.704 kb on + strand, within ABZR86_RS00030 at 5.705 kb on - strand, within ABZR86_RS00030 at 5.705 kb on - strand, within ABZR86_RS00030 at 5.705 kb on - strand, within ABZR86_RS00030 at 5.822 kb on - strand, within ABZR86_RS00030 at 5.848 kb on + strand, within ABZR86_RS00030 at 5.849 kb on - strand, within ABZR86_RS00030 at 5.849 kb on - strand, within ABZR86_RS00030 at 5.849 kb on - strand, within ABZR86_RS00030 at 6.294 kb on - strand at 6.294 kb on - strand at 6.423 kb on + strand at 6.423 kb on + strand at 6.423 kb on + strand at 6.424 kb on - strand at 6.424 kb on - strand at 6.424 kb on - strand at 6.424 kb on - strand at 6.493 kb on + strand at 7.097 kb on - strand, within ABZR86_RS00035 at 7.297 kb on + strand, within ABZR86_RS00035 at 7.297 kb on + strand, within ABZR86_RS00035 at 7.309 kb on + strand, within ABZR86_RS00035 at 7.336 kb on + strand, within ABZR86_RS00035 at 7.669 kb on + strand, within ABZR86_RS00035 at 7.738 kb on + strand, within ABZR86_RS00035 at 7.738 kb on + strand, within ABZR86_RS00035 at 7.739 kb on - strand, within ABZR86_RS00035 at 8.355 kb on - strand at 8.435 kb on + strand, within ABZR86_RS00040 at 8.435 kb on + strand, within ABZR86_RS00040 at 8.436 kb on - strand, within ABZR86_RS00040 at 8.526 kb on + strand, within ABZR86_RS00040 at 8.681 kb on + strand, within ABZR86_RS00040 at 8.815 kb on + strand, within ABZR86_RS00040 at 8.815 kb on + strand, within ABZR86_RS00040 at 8.815 kb on + strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.816 kb on - strand, within ABZR86_RS00040 at 8.871 kb on + strand, within ABZR86_RS00040 at 8.997 kb on - strand, within ABZR86_RS00040
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,519 - ABZR86_RS00030 0.64 +0.5 5,704 + ABZR86_RS00030 0.76 +1.5 5,705 - ABZR86_RS00030 0.76 -0.8 5,705 - ABZR86_RS00030 0.76 -0.1 5,705 - ABZR86_RS00030 0.76 -0.4 5,822 - ABZR86_RS00030 0.83 -0.9 5,848 + ABZR86_RS00030 0.85 +0.6 5,849 - ABZR86_RS00030 0.85 -0.2 5,849 - ABZR86_RS00030 0.85 +0.3 5,849 - ABZR86_RS00030 0.85 +0.3 6,294 - -0.0 6,294 - -1.5 6,423 + +0.5 6,423 + +0.9 6,423 + +0.6 6,424 - -1.4 6,424 - +0.1 6,424 - +0.9 6,424 - +0.3 6,493 + -0.6 7,097 - ABZR86_RS00035 0.42 +0.2 7,297 + ABZR86_RS00035 0.54 +0.7 7,297 + ABZR86_RS00035 0.54 +1.5 7,309 + ABZR86_RS00035 0.55 +1.3 7,336 + ABZR86_RS00035 0.56 +0.6 7,669 + ABZR86_RS00035 0.76 -1.1 7,738 + ABZR86_RS00035 0.80 -0.1 7,738 + ABZR86_RS00035 0.80 +0.7 7,739 - ABZR86_RS00035 0.80 +0.3 8,355 - +1.3 8,435 + ABZR86_RS00040 0.10 +0.2 8,435 + ABZR86_RS00040 0.10 +0.0 8,436 - ABZR86_RS00040 0.11 -0.4 8,526 + ABZR86_RS00040 0.19 -0.0 8,681 + ABZR86_RS00040 0.34 +0.7 8,815 + ABZR86_RS00040 0.47 +0.1 8,815 + ABZR86_RS00040 0.47 -0.3 8,815 + ABZR86_RS00040 0.47 -0.6 8,816 - ABZR86_RS00040 0.47 -0.2 8,816 - ABZR86_RS00040 0.47 +1.5 8,816 - ABZR86_RS00040 0.47 +0.9 8,816 - ABZR86_RS00040 0.47 +0.4 8,816 - ABZR86_RS00040 0.47 -0.3 8,816 - ABZR86_RS00040 0.47 -0.6 8,816 - ABZR86_RS00040 0.47 +1.5 8,871 + ABZR86_RS00040 0.52 -1.2 8,997 - ABZR86_RS00040 0.64 +0.4
Or see this region's nucleotide sequence