Experiment: 2-Furfuraldehyde 0.125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0044 and Shewana3_R0045 are separated by 43 nucleotides Shewana3_R0045 and Shewana3_R0046 are separated by 44 nucleotides Shewana3_R0046 and Shewana3_R0047 are separated by 36 nucleotides Shewana3_R0047 and Shewana3_R0048 are separated by 94 nucleotides Shewana3_R0048 and valS are separated by 406 nucleotides valS and Shewana3_1132 are separated by 15 nucleotides
Shewana3_R0044: Shewana3_R0044 - tRNA-Arg (RefSeq), at 1,333,015 to 1,333,091
_R0044
Shewana3_R0045: Shewana3_R0045 - tRNA-Arg (RefSeq), at 1,333,135 to 1,333,211
_R0045
Shewana3_R0046: Shewana3_R0046 - tRNA-Arg (RefSeq), at 1,333,256 to 1,333,332
_R0046
Shewana3_R0047: Shewana3_R0047 - tRNA-Ser (RefSeq), at 1,333,369 to 1,333,460
_R0047
Shewana3_R0048: Shewana3_R0048 - tRNA-Arg (RefSeq), at 1,333,555 to 1,333,631
_R0048
Shewana3_1131: valS - valyl-tRNA synthetase (RefSeq), at 1,334,038 to 1,336,914
valS
Shewana3_1132: Shewana3_1132 - DNA polymerase III chi subunit, HolC (RefSeq), at 1,336,930 to 1,337,394
_1132
Position (kb)
1334
1335
1336
1337 Strain fitness (log2 ratio)
-1
0
1 at 1333.713 kb on + strand at 1333.853 kb on + strand at 1333.901 kb on + strand at 1333.957 kb on + strand at 1333.957 kb on + strand at 1334.036 kb on - strand at 1334.050 kb on - strand at 1337.372 kb on + strand at 1337.380 kb on - strand at 1337.409 kb on + strand at 1337.520 kb on - strand at 1337.528 kb on + strand at 1337.537 kb on - strand at 1337.564 kb on - strand at 1337.601 kb on + strand at 1337.630 kb on - strand at 1337.632 kb on + strand at 1337.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.125 vol% remove 1,333,713 + +0.2 1,333,853 + -0.6 1,333,901 + +0.3 1,333,957 + -0.9 1,333,957 + +1.7 1,334,036 - +1.4 1,334,050 - +0.7 1,337,372 + +0.3 1,337,380 - +1.7 1,337,409 + -0.2 1,337,520 - +0.5 1,337,528 + +1.1 1,337,537 - +0.1 1,337,564 - -0.3 1,337,601 + -0.1 1,337,630 - -0.1 1,337,632 + -0.3 1,337,855 + +0.1
Or see this region's nucleotide sequence