Experiment: 2-Furfuraldehyde 0.125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipB and Shewana3_0990 are separated by 92 nucleotides Shewana3_0990 and Shewana3_0991 are separated by 121 nucleotides Shewana3_0991 and Shewana3_0992 are separated by 144 nucleotides Shewana3_0992 and Shewana3_0993 overlap by 17 nucleotides
Shewana3_0989: lipB - lipoyltransferase (RefSeq), at 1,159,865 to 1,160,518
lipB
Shewana3_0990: Shewana3_0990 - hypothetical protein (RefSeq), at 1,160,611 to 1,160,877
_0990
Shewana3_0991: Shewana3_0991 - penicillin-binding protein 6 (RefSeq), at 1,160,999 to 1,162,174
_0991
Shewana3_0992: Shewana3_0992 - rare lipoprotein A (RefSeq), at 1,162,319 to 1,163,161
_0992
Shewana3_0993: Shewana3_0993 - lytic murein transglycosylase B (RefSeq), at 1,163,145 to 1,164,209
_0993
Position (kb)
1160
1161
1162
1163 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1161.637 kb on - strand, within Shewana3_0991 at 1162.240 kb on - strand at 1162.310 kb on - strand at 1162.404 kb on - strand, within Shewana3_0992 at 1162.438 kb on + strand, within Shewana3_0992 at 1162.503 kb on - strand, within Shewana3_0992 at 1162.580 kb on - strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.801 kb on - strand, within Shewana3_0992 at 1162.902 kb on + strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.125 vol% remove 1,161,637 - Shewana3_0991 0.54 -2.8 1,162,240 - -2.0 1,162,310 - -0.9 1,162,404 - Shewana3_0992 0.10 +0.7 1,162,438 + Shewana3_0992 0.14 -0.3 1,162,503 - Shewana3_0992 0.22 -0.3 1,162,580 - Shewana3_0992 0.31 -1.4 1,162,605 + Shewana3_0992 0.34 +1.7 1,162,605 + Shewana3_0992 0.34 +0.7 1,162,801 - Shewana3_0992 0.57 -1.4 1,162,902 + Shewana3_0992 0.69 +1.7 1,162,910 - Shewana3_0992 0.70 -0.3 1,162,910 - Shewana3_0992 0.70 +0.7
Or see this region's nucleotide sequence