Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3773 and Shew_3774 are separated by 19 nucleotides Shew_3774 and Shew_3775 are separated by 5 nucleotides Shew_3775 and Shew_3776 are separated by 159 nucleotides Shew_3776 and Shew_3777 are separated by 127 nucleotides
Shew_3773: Shew_3773 - hypothetical protein (RefSeq), at 4,492,705 to 4,493,745
_3773
Shew_3774: Shew_3774 - acetyltransferase-like (RefSeq), at 4,493,765 to 4,494,091
_3774
Shew_3775: Shew_3775 - thioesterase superfamily protein (RefSeq), at 4,494,097 to 4,494,501
_3775
Shew_3776: Shew_3776 - AMP-dependent synthetase and ligase (RefSeq), at 4,494,661 to 4,496,457
_3776
Shew_3777: Shew_3777 - hypothetical protein (RefSeq), at 4,496,585 to 4,497,847
_3777
Position (kb)
4494
4495
4496
4497 Strain fitness (log2 ratio)
-2
-1
0
1 at 4493.812 kb on - strand, within Shew_3774 at 4493.819 kb on - strand, within Shew_3774 at 4494.028 kb on - strand, within Shew_3774 at 4494.039 kb on - strand, within Shew_3774 at 4494.039 kb on - strand, within Shew_3774 at 4494.182 kb on + strand, within Shew_3775 at 4494.196 kb on - strand, within Shew_3775 at 4494.461 kb on + strand at 4494.506 kb on + strand at 4494.645 kb on + strand at 4495.093 kb on - strand, within Shew_3776 at 4495.215 kb on + strand, within Shew_3776 at 4495.247 kb on + strand, within Shew_3776 at 4495.295 kb on + strand, within Shew_3776 at 4495.331 kb on - strand, within Shew_3776 at 4495.484 kb on + strand, within Shew_3776 at 4495.531 kb on - strand, within Shew_3776 at 4495.595 kb on + strand, within Shew_3776 at 4495.600 kb on + strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.613 kb on + strand, within Shew_3776 at 4495.621 kb on - strand, within Shew_3776 at 4495.621 kb on - strand, within Shew_3776 at 4495.658 kb on - strand, within Shew_3776 at 4496.090 kb on - strand, within Shew_3776 at 4496.241 kb on - strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.280 kb on - strand at 4496.371 kb on + strand at 4496.596 kb on - strand at 4496.789 kb on + strand, within Shew_3777 at 4496.789 kb on + strand, within Shew_3777 at 4496.925 kb on + strand, within Shew_3777 at 4496.995 kb on - strand, within Shew_3777 at 4496.996 kb on + strand, within Shew_3777 at 4497.041 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.067 kb on - strand, within Shew_3777 at 4497.072 kb on - strand, within Shew_3777 at 4497.163 kb on + strand, within Shew_3777 at 4497.319 kb on - strand, within Shew_3777 at 4497.455 kb on - strand, within Shew_3777
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 4,493,812 - Shew_3774 0.14 -0.0 4,493,819 - Shew_3774 0.17 -0.4 4,494,028 - Shew_3774 0.80 +0.2 4,494,039 - Shew_3774 0.84 -0.2 4,494,039 - Shew_3774 0.84 +1.1 4,494,182 + Shew_3775 0.21 +1.1 4,494,196 - Shew_3775 0.24 -0.4 4,494,461 + +0.2 4,494,506 + +0.2 4,494,645 + -0.2 4,495,093 - Shew_3776 0.24 +0.2 4,495,215 + Shew_3776 0.31 +0.3 4,495,247 + Shew_3776 0.33 +0.2 4,495,295 + Shew_3776 0.35 -0.4 4,495,331 - Shew_3776 0.37 -0.7 4,495,484 + Shew_3776 0.46 -0.4 4,495,531 - Shew_3776 0.48 -2.5 4,495,595 + Shew_3776 0.52 +0.7 4,495,600 + Shew_3776 0.52 -0.1 4,495,608 - Shew_3776 0.53 -0.2 4,495,608 - Shew_3776 0.53 -0.2 4,495,608 - Shew_3776 0.53 +0.2 4,495,613 + Shew_3776 0.53 -0.3 4,495,621 - Shew_3776 0.53 -0.4 4,495,621 - Shew_3776 0.53 -0.2 4,495,658 - Shew_3776 0.55 -0.6 4,496,090 - Shew_3776 0.80 -0.1 4,496,241 - Shew_3776 0.88 +0.1 4,496,272 + Shew_3776 0.90 -0.6 4,496,272 + Shew_3776 0.90 -0.9 4,496,272 + Shew_3776 0.90 +0.1 4,496,280 - -0.2 4,496,371 + -0.5 4,496,596 - +0.2 4,496,789 + Shew_3777 0.16 -0.9 4,496,789 + Shew_3777 0.16 +0.8 4,496,925 + Shew_3777 0.27 -1.5 4,496,995 - Shew_3777 0.32 -0.5 4,496,996 + Shew_3777 0.33 -0.4 4,497,041 + Shew_3777 0.36 -0.0 4,497,054 + Shew_3777 0.37 +0.4 4,497,054 + Shew_3777 0.37 -0.8 4,497,054 + Shew_3777 0.37 +0.1 4,497,067 - Shew_3777 0.38 +1.7 4,497,072 - Shew_3777 0.39 -0.2 4,497,163 + Shew_3777 0.46 +0.1 4,497,319 - Shew_3777 0.58 +1.7 4,497,455 - Shew_3777 0.69 -0.2
Or see this region's nucleotide sequence