Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3704 and Shew_3705 are separated by 28 nucleotides Shew_3705 and Shew_3706 are separated by 65 nucleotides Shew_3706 and Shew_3707 are separated by 27 nucleotides
Shew_3704: Shew_3704 - dTDP-glucose 4,6-dehydratase (RefSeq), at 4,408,268 to 4,409,338
_3704
Shew_3705: Shew_3705 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 4,409,367 to 4,410,296
_3705
Shew_3706: Shew_3706 - 3-deoxy-D-manno-octulosonic-acid kinase (RefSeq), at 4,410,362 to 4,411,087
_3706
Shew_3707: Shew_3707 - 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein (RefSeq), at 4,411,115 to 4,412,380
_3707
Position (kb) 4409
4410
4411 Strain fitness (log2 ratio) -1
0
1 at 4408.390 kb on - strand, within Shew_3704 at 4408.392 kb on + strand, within Shew_3704 at 4408.493 kb on - strand, within Shew_3704 at 4408.611 kb on + strand, within Shew_3704 at 4408.640 kb on - strand, within Shew_3704 at 4408.915 kb on + strand, within Shew_3704 at 4408.985 kb on - strand, within Shew_3704 at 4408.998 kb on + strand, within Shew_3704 at 4409.006 kb on - strand, within Shew_3704 at 4409.422 kb on - strand at 4409.630 kb on - strand, within Shew_3705 at 4409.936 kb on + strand, within Shew_3705 at 4409.992 kb on + strand, within Shew_3705 at 4411.081 kb on + strand at 4411.089 kb on - strand at 4411.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 4,408,390 - Shew_3704 0.11 +0.0 4,408,392 + Shew_3704 0.12 -0.4 4,408,493 - Shew_3704 0.21 +0.7 4,408,611 + Shew_3704 0.32 +0.0 4,408,640 - Shew_3704 0.35 +1.1 4,408,915 + Shew_3704 0.60 -0.1 4,408,985 - Shew_3704 0.67 -0.3 4,408,998 + Shew_3704 0.68 +0.4 4,409,006 - Shew_3704 0.69 +0.1 4,409,422 - +0.2 4,409,630 - Shew_3705 0.28 -0.1 4,409,936 + Shew_3705 0.61 -0.3 4,409,992 + Shew_3705 0.67 -0.6 4,411,081 + +0.3 4,411,089 - -0.1 4,411,089 - +0.9
Or see this region's nucleotide sequence