Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3053 and Shew_3054 are separated by 206 nucleotides Shew_3054 and Shew_3055 are separated by 255 nucleotides Shew_3055 and Shew_3056 are separated by 140 nucleotides
Shew_3053: Shew_3053 - Serine-type D-Ala-D-Ala carboxypeptidase (RefSeq), at 3,625,140 to 3,626,402
_3053
Shew_3054: Shew_3054 - hypothetical protein (RefSeq), at 3,626,609 to 3,627,187
_3054
Shew_3055: Shew_3055 - CMP/dCMP deaminase, zinc-binding (RefSeq), at 3,627,443 to 3,627,892
_3055
Shew_3056: Shew_3056 - hypothetical protein (RefSeq), at 3,628,033 to 3,628,605
_3056
Position (kb)
3626
3627
3628 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3625.843 kb on - strand, within Shew_3053 at 3625.897 kb on + strand, within Shew_3053 at 3626.128 kb on - strand, within Shew_3053 at 3626.353 kb on + strand at 3626.362 kb on + strand at 3626.370 kb on - strand at 3626.491 kb on + strand at 3626.627 kb on - strand at 3626.666 kb on + strand at 3626.789 kb on + strand, within Shew_3054 at 3627.006 kb on - strand, within Shew_3054 at 3627.300 kb on - strand at 3627.316 kb on - strand at 3627.342 kb on + strand at 3627.750 kb on + strand, within Shew_3055 at 3627.768 kb on + strand, within Shew_3055 at 3627.809 kb on - strand, within Shew_3055 at 3627.839 kb on - strand, within Shew_3055 at 3627.868 kb on + strand at 3627.868 kb on + strand at 3627.885 kb on - strand at 3627.887 kb on + strand at 3627.887 kb on + strand at 3627.895 kb on - strand at 3627.895 kb on - strand at 3627.895 kb on - strand at 3627.895 kb on - strand at 3627.897 kb on + strand at 3627.944 kb on + strand at 3628.134 kb on + strand, within Shew_3056 at 3628.139 kb on + strand, within Shew_3056 at 3628.142 kb on - strand, within Shew_3056 at 3628.147 kb on - strand, within Shew_3056 at 3628.147 kb on - strand, within Shew_3056 at 3628.164 kb on + strand, within Shew_3056 at 3628.172 kb on - strand, within Shew_3056 at 3628.185 kb on - strand, within Shew_3056 at 3628.187 kb on - strand, within Shew_3056
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,625,843 - Shew_3053 0.56 -0.1 3,625,897 + Shew_3053 0.60 +0.3 3,626,128 - Shew_3053 0.78 +0.3 3,626,353 + +0.2 3,626,362 + -0.0 3,626,370 - -0.3 3,626,491 + -1.7 3,626,627 - -0.0 3,626,666 + +0.0 3,626,789 + Shew_3054 0.31 +0.9 3,627,006 - Shew_3054 0.69 -0.1 3,627,300 - -0.1 3,627,316 - -0.7 3,627,342 + +0.2 3,627,750 + Shew_3055 0.68 -3.8 3,627,768 + Shew_3055 0.72 -3.7 3,627,809 - Shew_3055 0.81 -3.2 3,627,839 - Shew_3055 0.88 -3.9 3,627,868 + +0.2 3,627,868 + -0.2 3,627,885 - +0.8 3,627,887 + +0.7 3,627,887 + +0.4 3,627,895 - -0.0 3,627,895 - +0.0 3,627,895 - -2.8 3,627,895 - +0.1 3,627,897 + +0.0 3,627,944 + +0.7 3,628,134 + Shew_3056 0.18 +0.7 3,628,139 + Shew_3056 0.18 -0.2 3,628,142 - Shew_3056 0.19 -0.0 3,628,147 - Shew_3056 0.20 -0.1 3,628,147 - Shew_3056 0.20 +0.7 3,628,164 + Shew_3056 0.23 +0.1 3,628,172 - Shew_3056 0.24 -0.3 3,628,185 - Shew_3056 0.27 +0.4 3,628,187 - Shew_3056 0.27 -0.0
Or see this region's nucleotide sequence