Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2965 and Shew_2966 are separated by 119 nucleotides Shew_2966 and Shew_2967 are separated by 229 nucleotides Shew_2967 and Shew_2968 are separated by 246 nucleotides Shew_2968 and Shew_2969 are separated by 116 nucleotides
Shew_2965: Shew_2965 - putative manganese transporter (RefSeq), at 3,517,568 to 3,518,836
_2965
Shew_2966: Shew_2966 - membrane-flanked domain-containing protein (RefSeq), at 3,518,956 to 3,519,486
_2966
Shew_2967: Shew_2967 - TonB-dependent receptor (RefSeq), at 3,519,716 to 3,522,289
_2967
Shew_2968: Shew_2968 - glutathione S-transferase domain-containing protein (RefSeq), at 3,522,536 to 3,523,171
_2968
Shew_2969: Shew_2969 - hypothetical protein (RefSeq), at 3,523,288 to 3,523,908
_2969
Position (kb)
3519
3520
3521
3522
3523 Strain fitness (log2 ratio)
-1
0
1
2 at 3519.064 kb on - strand, within Shew_2966 at 3519.707 kb on + strand at 3519.774 kb on + strand at 3519.973 kb on - strand at 3519.992 kb on - strand, within Shew_2967 at 3520.215 kb on - strand, within Shew_2967 at 3520.299 kb on + strand, within Shew_2967 at 3520.307 kb on - strand, within Shew_2967 at 3520.307 kb on - strand, within Shew_2967 at 3520.307 kb on - strand, within Shew_2967 at 3520.930 kb on - strand, within Shew_2967 at 3520.930 kb on - strand, within Shew_2967 at 3521.340 kb on + strand, within Shew_2967 at 3521.341 kb on + strand, within Shew_2967 at 3521.541 kb on + strand, within Shew_2967 at 3521.634 kb on + strand, within Shew_2967 at 3521.634 kb on + strand, within Shew_2967 at 3521.684 kb on - strand, within Shew_2967 at 3522.238 kb on + strand at 3522.238 kb on + strand at 3522.246 kb on - strand at 3522.414 kb on - strand at 3522.511 kb on + strand at 3523.115 kb on + strand at 3523.161 kb on + strand at 3523.200 kb on + strand at 3523.200 kb on + strand at 3523.208 kb on - strand at 3523.218 kb on - strand at 3523.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,519,064 - Shew_2966 0.20 +0.0 3,519,707 + +0.8 3,519,774 + +0.9 3,519,973 - +0.6 3,519,992 - Shew_2967 0.11 -0.9 3,520,215 - Shew_2967 0.19 +1.2 3,520,299 + Shew_2967 0.23 +0.9 3,520,307 - Shew_2967 0.23 +1.5 3,520,307 - Shew_2967 0.23 +0.1 3,520,307 - Shew_2967 0.23 +0.1 3,520,930 - Shew_2967 0.47 +0.3 3,520,930 - Shew_2967 0.47 +0.4 3,521,340 + Shew_2967 0.63 +1.8 3,521,341 + Shew_2967 0.63 +0.0 3,521,541 + Shew_2967 0.71 +0.2 3,521,634 + Shew_2967 0.75 -0.2 3,521,634 + Shew_2967 0.75 +0.3 3,521,684 - Shew_2967 0.76 -0.0 3,522,238 + +0.0 3,522,238 + +0.2 3,522,246 - -0.3 3,522,414 - -1.5 3,522,511 + +0.1 3,523,115 + -0.5 3,523,161 + -0.1 3,523,200 + -0.6 3,523,200 + -0.1 3,523,208 - -1.1 3,523,218 - -0.1 3,523,255 + -0.6
Or see this region's nucleotide sequence