Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2641 and Shew_2642 overlap by 49 nucleotides Shew_2642 and Shew_2643 are separated by 373 nucleotides Shew_2643 and Shew_2644 are separated by 10 nucleotides Shew_2644 and Shew_2645 are separated by 183 nucleotides
Shew_2641: Shew_2641 - PTS system, glucose-like IIB subunint (RefSeq), at 3,116,519 to 3,117,991
_2641
Shew_2642: Shew_2642 - flavodoxin (RefSeq), at 3,117,943 to 3,118,407
_2642
Shew_2643: Shew_2643 - pseudouridylate synthase (RefSeq), at 3,118,781 to 3,119,602
_2643
Shew_2644: Shew_2644 - hypothetical protein (RefSeq), at 3,119,613 to 3,119,930
_2644
Shew_2645: Shew_2645 - hypothetical protein (RefSeq), at 3,120,114 to 3,121,127
_2645
Position (kb)
3118
3119
3120 Strain fitness (log2 ratio)
-1
0
1 at 3118.241 kb on - strand, within Shew_2642 at 3119.245 kb on + strand, within Shew_2643 at 3119.717 kb on - strand, within Shew_2644 at 3119.814 kb on + strand, within Shew_2644 at 3120.009 kb on + strand at 3120.224 kb on + strand, within Shew_2645 at 3120.250 kb on - strand, within Shew_2645 at 3120.451 kb on - strand, within Shew_2645 at 3120.508 kb on - strand, within Shew_2645 at 3120.584 kb on - strand, within Shew_2645 at 3120.589 kb on - strand, within Shew_2645 at 3120.598 kb on + strand, within Shew_2645
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,118,241 - Shew_2642 0.64 +1.7 3,119,245 + Shew_2643 0.56 -1.0 3,119,717 - Shew_2644 0.33 -0.0 3,119,814 + Shew_2644 0.63 -0.1 3,120,009 + +0.7 3,120,224 + Shew_2645 0.11 +0.0 3,120,250 - Shew_2645 0.13 +0.0 3,120,451 - Shew_2645 0.33 +0.1 3,120,508 - Shew_2645 0.39 -0.1 3,120,584 - Shew_2645 0.46 +0.4 3,120,589 - Shew_2645 0.47 +0.1 3,120,598 + Shew_2645 0.48 +0.0
Or see this region's nucleotide sequence