Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1231 and Shew_1232 overlap by 1 nucleotides Shew_1232 and Shew_1233 are separated by 50 nucleotides Shew_1233 and Shew_1234 are separated by 99 nucleotides
Shew_1231: Shew_1231 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 1,430,464 to 1,431,057
_1231
Shew_1232: Shew_1232 - hypothetical protein (RefSeq), at 1,431,057 to 1,431,824
_1232
Shew_1233: Shew_1233 - hypothetical protein (RefSeq), at 1,431,875 to 1,432,366
_1233
Shew_1234: Shew_1234 - peptidase S41 (RefSeq), at 1,432,466 to 1,435,780
_1234
Position (kb)
1431
1432 Strain fitness (log2 ratio)
-1
0
1
2 at 1430.409 kb on + strand at 1430.614 kb on - strand, within Shew_1231 at 1430.677 kb on - strand, within Shew_1231 at 1431.131 kb on + strand at 1431.618 kb on + strand, within Shew_1232 at 1431.618 kb on + strand, within Shew_1232 at 1431.626 kb on - strand, within Shew_1232 at 1431.694 kb on - strand, within Shew_1232 at 1431.987 kb on - strand, within Shew_1233 at 1432.113 kb on - strand, within Shew_1233 at 1432.136 kb on + strand, within Shew_1233 at 1432.193 kb on - strand, within Shew_1233 at 1432.597 kb on - strand at 1432.780 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 1,430,409 + -0.7 1,430,614 - Shew_1231 0.25 +1.0 1,430,677 - Shew_1231 0.36 +0.1 1,431,131 + +0.7 1,431,618 + Shew_1232 0.73 +0.7 1,431,618 + Shew_1232 0.73 +0.5 1,431,626 - Shew_1232 0.74 +0.3 1,431,694 - Shew_1232 0.83 +2.5 1,431,987 - Shew_1233 0.23 +0.0 1,432,113 - Shew_1233 0.48 +0.4 1,432,136 + Shew_1233 0.53 +0.1 1,432,193 - Shew_1233 0.65 +1.4 1,432,597 - +0.6 1,432,780 + +1.0
Or see this region's nucleotide sequence