Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0527 and Shew_0528 are separated by 94 nucleotides Shew_0528 and Shew_0529 are separated by 79 nucleotides Shew_0529 and Shew_0530 are separated by 233 nucleotides
Shew_0527: Shew_0527 - Sel1 domain-containing protein (RefSeq), at 596,449 to 597,303
_0527
Shew_0528: Shew_0528 - Sel1 domain-containing protein (RefSeq), at 597,398 to 598,222
_0528
Shew_0529: Shew_0529 - Sel1 domain-containing protein (RefSeq), at 598,302 to 599,129
_0529
Shew_0530: Shew_0530 - FAD dependent oxidoreductase (RefSeq), at 599,363 to 600,490
_0530
Position (kb) 598
599
600 Strain fitness (log2 ratio) -1
0
1 at 597.444 kb on - strand at 597.450 kb on - strand at 597.887 kb on - strand, within Shew_0528 at 597.945 kb on + strand, within Shew_0528 at 597.953 kb on - strand, within Shew_0528 at 597.992 kb on - strand, within Shew_0528 at 598.180 kb on + strand at 598.698 kb on - strand, within Shew_0529 at 599.062 kb on + strand at 599.219 kb on + strand at 599.308 kb on + strand at 599.491 kb on - strand, within Shew_0530 at 599.562 kb on - strand, within Shew_0530 at 599.566 kb on + strand, within Shew_0530 at 599.819 kb on - strand, within Shew_0530 at 599.993 kb on - strand, within Shew_0530 at 600.012 kb on + strand, within Shew_0530
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 597,444 - -0.2 597,450 - -1.0 597,887 - Shew_0528 0.59 +1.0 597,945 + Shew_0528 0.66 +0.4 597,953 - Shew_0528 0.67 +0.0 597,992 - Shew_0528 0.72 -1.2 598,180 + +0.0 598,698 - Shew_0529 0.48 -0.7 599,062 + -0.6 599,219 + +0.2 599,308 + -0.0 599,491 - Shew_0530 0.11 +0.4 599,562 - Shew_0530 0.18 -0.3 599,566 + Shew_0530 0.18 +0.5 599,819 - Shew_0530 0.40 +0.5 599,993 - Shew_0530 0.56 -0.2 600,012 + Shew_0530 0.58 -0.1
Or see this region's nucleotide sequence