Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0432 and Shew_0433 are separated by 21 nucleotides Shew_0433 and Shew_0434 are separated by 92 nucleotides Shew_0434 and Shew_0435 are separated by 247 nucleotides Shew_0435 and Shew_0436 are separated by 14 nucleotides
Shew_0432: Shew_0432 - hypothetical protein (RefSeq), at 484,163 to 484,672
_0432
Shew_0433: Shew_0433 - hypothetical protein (RefSeq), at 484,694 to 485,083
_0433
Shew_0434: Shew_0434 - hypothetical protein (RefSeq), at 485,176 to 485,487
_0434
Shew_0435: Shew_0435 - hypothetical protein (RefSeq), at 485,735 to 486,256
_0435
Shew_0436: Shew_0436 - diguanylate cyclase/phosphodiesterase (RefSeq), at 486,271 to 488,043
_0436
Position (kb) 485
486 Strain fitness (log2 ratio) -1
0
1 at 484.237 kb on + strand, within Shew_0432 at 484.417 kb on + strand, within Shew_0432 at 484.585 kb on + strand, within Shew_0432 at 484.774 kb on + strand, within Shew_0433 at 484.781 kb on - strand, within Shew_0433 at 485.365 kb on + strand, within Shew_0434 at 485.406 kb on + strand, within Shew_0434 at 485.698 kb on - strand at 486.035 kb on - strand, within Shew_0435 at 486.333 kb on + strand at 486.443 kb on - strand at 486.482 kb on - strand, within Shew_0436
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 484,237 + Shew_0432 0.15 -0.1 484,417 + Shew_0432 0.50 +0.8 484,585 + Shew_0432 0.83 +0.2 484,774 + Shew_0433 0.21 -1.3 484,781 - Shew_0433 0.22 -0.2 485,365 + Shew_0434 0.61 +0.0 485,406 + Shew_0434 0.74 -1.5 485,698 - +0.5 486,035 - Shew_0435 0.57 +0.0 486,333 + +0.5 486,443 - +0.7 486,482 - Shew_0436 0.12 +0.3
Or see this region's nucleotide sequence