Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0068 and Shew_0069 are separated by 44 nucleotides Shew_0069 and Shew_0070 are separated by 178 nucleotides
Shew_0068: Shew_0068 - fumarate lyase (RefSeq), at 80,976 to 82,355
_0068
Shew_0069: Shew_0069 - hypothetical protein (RefSeq), at 82,400 to 83,503
_0069
Shew_0070: Shew_0070 - iron-containing alcohol dehydrogenase (RefSeq), at 83,682 to 84,845
_0070
Position (kb) 82
83
84 Strain fitness (log2 ratio) -1
0
1 at 81.407 kb on - strand, within Shew_0068 at 81.690 kb on - strand, within Shew_0068 at 81.797 kb on + strand, within Shew_0068 at 81.871 kb on + strand, within Shew_0068 at 81.888 kb on - strand, within Shew_0068 at 81.937 kb on - strand, within Shew_0068 at 82.068 kb on - strand, within Shew_0068 at 82.195 kb on + strand, within Shew_0068 at 82.386 kb on + strand at 82.389 kb on - strand at 82.413 kb on + strand at 82.416 kb on - strand at 82.471 kb on + strand at 82.471 kb on + strand at 82.671 kb on - strand, within Shew_0069 at 82.671 kb on - strand, within Shew_0069 at 82.853 kb on - strand, within Shew_0069 at 82.881 kb on - strand, within Shew_0069 at 83.228 kb on + strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.235 kb on - strand, within Shew_0069 at 83.309 kb on - strand, within Shew_0069 at 83.438 kb on - strand at 83.453 kb on + strand at 83.749 kb on + strand at 83.931 kb on - strand, within Shew_0070 at 83.946 kb on + strand, within Shew_0070 at 83.972 kb on - strand, within Shew_0070 at 84.106 kb on + strand, within Shew_0070 at 84.204 kb on + strand, within Shew_0070 at 84.270 kb on - strand, within Shew_0070 at 84.282 kb on - strand, within Shew_0070
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 81,407 - Shew_0068 0.31 -0.0 81,690 - Shew_0068 0.52 -0.1 81,797 + Shew_0068 0.59 -0.9 81,871 + Shew_0068 0.65 -0.7 81,888 - Shew_0068 0.66 -1.3 81,937 - Shew_0068 0.70 -0.1 82,068 - Shew_0068 0.79 +0.0 82,195 + Shew_0068 0.88 -0.1 82,386 + +0.2 82,389 - +0.0 82,413 + +0.1 82,416 - -0.4 82,471 + -1.1 82,471 + +0.2 82,671 - Shew_0069 0.25 +0.3 82,671 - Shew_0069 0.25 -0.4 82,853 - Shew_0069 0.41 +0.1 82,881 - Shew_0069 0.44 -0.3 83,228 + Shew_0069 0.75 -0.4 83,235 - Shew_0069 0.76 +0.1 83,235 - Shew_0069 0.76 -0.3 83,309 - Shew_0069 0.82 -0.0 83,438 - -0.2 83,453 + -0.3 83,749 + +0.2 83,931 - Shew_0070 0.21 -0.3 83,946 + Shew_0070 0.23 -0.2 83,972 - Shew_0070 0.25 +0.0 84,106 + Shew_0070 0.36 -0.3 84,204 + Shew_0070 0.45 -1.7 84,270 - Shew_0070 0.51 +0.6 84,282 - Shew_0070 0.52 -0.7
Or see this region's nucleotide sequence