Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0049 and Shew_0050 are separated by 103 nucleotides Shew_0050 and Shew_0051 are separated by 7 nucleotides Shew_0051 and Shew_0052 are separated by 95 nucleotides
Shew_0049: Shew_0049 - choline/carnitine/betaine transporter (RefSeq), at 59,401 to 60,972
_0049
Shew_0050: Shew_0050 - DNA polymerase III subunit epsilon (RefSeq), at 61,076 to 61,795
_0050
Shew_0051: Shew_0051 - cyclic nucleotide-binding protein (RefSeq), at 61,803 to 63,692
_0051
Shew_0052: Shew_0052 - DNA binding domain-containing protein (RefSeq), at 63,788 to 64,693
_0052
Position (kb) 61
62
63
64 Strain fitness (log2 ratio) -2
-1
0
1 at 60.950 kb on - strand at 60.950 kb on - strand at 61.064 kb on - strand at 61.187 kb on + strand, within Shew_0050 at 61.198 kb on - strand, within Shew_0050 at 61.204 kb on - strand, within Shew_0050 at 61.308 kb on + strand, within Shew_0050 at 61.473 kb on - strand, within Shew_0050 at 61.513 kb on + strand, within Shew_0050 at 61.572 kb on + strand, within Shew_0050 at 61.599 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.736 kb on - strand at 61.812 kb on - strand at 61.921 kb on + strand at 62.300 kb on + strand, within Shew_0051 at 62.300 kb on + strand, within Shew_0051 at 62.574 kb on + strand, within Shew_0051 at 62.695 kb on - strand, within Shew_0051 at 62.769 kb on + strand, within Shew_0051 at 62.778 kb on - strand, within Shew_0051 at 62.778 kb on - strand, within Shew_0051 at 62.993 kb on + strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.001 kb on - strand, within Shew_0051 at 63.141 kb on - strand, within Shew_0051 at 63.293 kb on + strand, within Shew_0051 at 63.301 kb on - strand, within Shew_0051 at 63.301 kb on - strand, within Shew_0051 at 63.303 kb on + strand, within Shew_0051 at 63.333 kb on + strand, within Shew_0051 at 63.426 kb on - strand, within Shew_0051 at 63.474 kb on + strand, within Shew_0051 at 63.474 kb on + strand, within Shew_0051 at 63.526 kb on + strand at 63.806 kb on - strand at 63.834 kb on + strand at 63.836 kb on + strand at 64.081 kb on + strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.089 kb on - strand, within Shew_0052 at 64.094 kb on - strand, within Shew_0052 at 64.284 kb on + strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.292 kb on - strand, within Shew_0052 at 64.326 kb on + strand, within Shew_0052 at 64.334 kb on + strand, within Shew_0052 at 64.611 kb on + strand at 64.619 kb on - strand at 64.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 60,950 - -0.2 60,950 - -0.1 61,064 - +0.2 61,187 + Shew_0050 0.15 -1.0 61,198 - Shew_0050 0.17 -0.0 61,204 - Shew_0050 0.18 -0.6 61,308 + Shew_0050 0.32 +1.1 61,473 - Shew_0050 0.55 +0.1 61,513 + Shew_0050 0.61 -0.4 61,572 + Shew_0050 0.69 -0.1 61,599 + Shew_0050 0.73 -0.1 61,616 + Shew_0050 0.75 -1.1 61,616 + Shew_0050 0.75 +0.8 61,736 - -0.0 61,812 - -0.1 61,921 + -0.7 62,300 + Shew_0051 0.26 -2.2 62,300 + Shew_0051 0.26 +0.1 62,574 + Shew_0051 0.41 +1.2 62,695 - Shew_0051 0.47 -0.4 62,769 + Shew_0051 0.51 -0.3 62,778 - Shew_0051 0.52 +0.1 62,778 - Shew_0051 0.52 -1.2 62,993 + Shew_0051 0.63 +0.1 63,001 - Shew_0051 0.63 +0.3 63,001 - Shew_0051 0.63 +0.2 63,001 - Shew_0051 0.63 -0.6 63,141 - Shew_0051 0.71 +0.3 63,293 + Shew_0051 0.79 -1.5 63,301 - Shew_0051 0.79 -0.0 63,301 - Shew_0051 0.79 -1.8 63,303 + Shew_0051 0.79 +0.5 63,333 + Shew_0051 0.81 -1.6 63,426 - Shew_0051 0.86 +0.3 63,474 + Shew_0051 0.88 -0.2 63,474 + Shew_0051 0.88 -0.4 63,526 + +0.1 63,806 - -0.1 63,834 + -0.0 63,836 + +0.5 64,081 + Shew_0052 0.32 -0.5 64,089 - Shew_0052 0.33 -0.1 64,089 - Shew_0052 0.33 -0.8 64,094 - Shew_0052 0.34 -0.0 64,284 + Shew_0052 0.55 -0.4 64,292 - Shew_0052 0.56 +0.1 64,292 - Shew_0052 0.56 -0.2 64,326 + Shew_0052 0.59 -0.1 64,334 + Shew_0052 0.60 -0.3 64,611 + +0.2 64,619 - -0.2 64,673 - +0.5
Or see this region's nucleotide sequence