Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_674

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotidesPf6N2E2_673 and Pf6N2E2_674 are separated by 12 nucleotidesPf6N2E2_674 and Pf6N2E2_675 overlap by 8 nucleotides Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017 _672 Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660 _673 Pf6N2E2_674 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 778,673 to 779,338 _674 Pf6N2E2_675 - 2-Methylcitrate dehydratase AcnD, at 779,331 to 781,106 _675 Position (kb) 778 779 780Strain fitness (log2 ratio) -2 -1 0 1at 778.078 kb on - strand, within Pf6N2E2_673at 778.078 kb on - strand, within Pf6N2E2_673at 778.081 kb on - strand, within Pf6N2E2_673at 778.282 kb on - strand, within Pf6N2E2_673at 778.282 kb on - strand, within Pf6N2E2_673at 778.375 kb on - strand, within Pf6N2E2_673at 778.623 kb on + strandat 778.624 kb on - strandat 778.651 kb on + strandat 778.651 kb on + strandat 778.652 kb on - strandat 778.740 kb on + strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.765 kb on - strand, within Pf6N2E2_674at 778.765 kb on - strand, within Pf6N2E2_674at 778.794 kb on + strand, within Pf6N2E2_674at 778.795 kb on - strand, within Pf6N2E2_674at 778.891 kb on - strand, within Pf6N2E2_674at 778.891 kb on - strand, within Pf6N2E2_674at 779.106 kb on + strand, within Pf6N2E2_674at 779.107 kb on - strand, within Pf6N2E2_674at 779.115 kb on + strand, within Pf6N2E2_674at 779.115 kb on + strand, within Pf6N2E2_674at 779.116 kb on - strand, within Pf6N2E2_674at 779.116 kb on - strand, within Pf6N2E2_674at 779.204 kb on + strand, within Pf6N2E2_674at 779.228 kb on + strand, within Pf6N2E2_674at 779.229 kb on - strand, within Pf6N2E2_674at 779.328 kb on + strandat 779.329 kb on - strandat 779.329 kb on - strandat 779.471 kb on - strandat 779.471 kb on - strandat 779.480 kb on - strandat 779.480 kb on - strandat 779.480 kb on - strandat 779.606 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.657 kb on - strand, within Pf6N2E2_675at 779.739 kb on + strand, within Pf6N2E2_675at 779.739 kb on + strand, within Pf6N2E2_675at 779.739 kb on + strand, within Pf6N2E2_675at 779.739 kb on + strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.740 kb on - strand, within Pf6N2E2_675at 779.973 kb on + strand, within Pf6N2E2_675at 779.973 kb on + strand, within Pf6N2E2_675at 779.973 kb on + strand, within Pf6N2E2_675at 779.973 kb on + strand, within Pf6N2E2_675at 779.974 kb on - strand, within Pf6N2E2_675at 779.974 kb on - strand, within Pf6N2E2_675at 780.197 kb on - strand, within Pf6N2E2_675at 780.263 kb on - strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.304 kb on + strand, within Pf6N2E2_675at 780.305 kb on - strand, within Pf6N2E2_675

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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778,078 - Pf6N2E2_673 0.10 -0.9
778,078 - Pf6N2E2_673 0.10 -0.5
778,081 - Pf6N2E2_673 0.11 +0.5
778,282 - Pf6N2E2_673 0.42 +1.0
778,282 - Pf6N2E2_673 0.42 -1.0
778,375 - Pf6N2E2_673 0.56 -2.5
778,623 + +0.3
778,624 - -1.1
778,651 + -0.9
778,651 + +0.7
778,652 - -2.0
778,740 + Pf6N2E2_674 0.10 -0.4
778,741 - Pf6N2E2_674 0.10 -1.3
778,741 - Pf6N2E2_674 0.10 +1.1
778,741 - Pf6N2E2_674 0.10 -1.8
778,741 - Pf6N2E2_674 0.10 -2.0
778,765 - Pf6N2E2_674 0.14 +0.2
778,765 - Pf6N2E2_674 0.14 +0.5
778,794 + Pf6N2E2_674 0.18 -1.2
778,795 - Pf6N2E2_674 0.18 -1.8
778,891 - Pf6N2E2_674 0.33 -0.5
778,891 - Pf6N2E2_674 0.33 -1.5
779,106 + Pf6N2E2_674 0.65 -1.1
779,107 - Pf6N2E2_674 0.65 +0.4
779,115 + Pf6N2E2_674 0.66 +0.3
779,115 + Pf6N2E2_674 0.66 +0.6
779,116 - Pf6N2E2_674 0.67 -2.1
779,116 - Pf6N2E2_674 0.67 -1.3
779,204 + Pf6N2E2_674 0.80 -2.2
779,228 + Pf6N2E2_674 0.83 -1.4
779,229 - Pf6N2E2_674 0.83 +1.0
779,328 + -0.2
779,329 - -0.2
779,329 - -0.5
779,471 - -0.5
779,471 - +1.2
779,480 - -0.4
779,480 - -0.4
779,480 - -2.2
779,606 + Pf6N2E2_675 0.15 -0.6
779,656 + Pf6N2E2_675 0.18 +0.5
779,656 + Pf6N2E2_675 0.18 +0.2
779,656 + Pf6N2E2_675 0.18 -0.0
779,656 + Pf6N2E2_675 0.18 +1.4
779,657 - Pf6N2E2_675 0.18 -2.6
779,739 + Pf6N2E2_675 0.23 -1.8
779,739 + Pf6N2E2_675 0.23 -1.2
779,739 + Pf6N2E2_675 0.23 +0.5
779,739 + Pf6N2E2_675 0.23 +1.6
779,740 - Pf6N2E2_675 0.23 -0.2
779,740 - Pf6N2E2_675 0.23 -0.0
779,740 - Pf6N2E2_675 0.23 +0.3
779,740 - Pf6N2E2_675 0.23 -2.6
779,740 - Pf6N2E2_675 0.23 -0.3
779,740 - Pf6N2E2_675 0.23 +0.5
779,973 + Pf6N2E2_675 0.36 -0.7
779,973 + Pf6N2E2_675 0.36 +0.3
779,973 + Pf6N2E2_675 0.36 -0.6
779,973 + Pf6N2E2_675 0.36 +0.6
779,974 - Pf6N2E2_675 0.36 -0.5
779,974 - Pf6N2E2_675 0.36 +1.4
780,197 - Pf6N2E2_675 0.49 +0.5
780,263 - Pf6N2E2_675 0.52 -1.7
780,304 + Pf6N2E2_675 0.55 +0.4
780,304 + Pf6N2E2_675 0.55 +0.6
780,304 + Pf6N2E2_675 0.55 -0.3
780,304 + Pf6N2E2_675 0.55 +0.4
780,304 + Pf6N2E2_675 0.55 -1.7
780,304 + Pf6N2E2_675 0.55 +0.7
780,305 - Pf6N2E2_675 0.55 -0.2

Or see this region's nucleotide sequence